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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 4: 804 - 812

DOI: https://doi.org/10.19852/j.cnki.jtcm.2024.04.001

Original articles

Assessment of phytochemicals, antioxidant, anti-hemolytic, anti-inflammatory and anti-cancer potential of flowers, leaves and stem extracts of Rosa arvensis

  • Tul Ain Zubaria ,
  • Fatima Iram ,
  • Naseer Sana ,
  • Kanwal Sobia ,
  • Mahmood Tariq
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  • 1 Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan
    2 Department of Biology, Allama Iqbal Open University, Islamabad 04403, Pakistan
    3 Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan
    4 Pakistan Academy of Sciences, Islamabad 04403, Pakistan

Received date: 2022-12-02

  Accepted date: 2023-04-07

  Online published: 2024-07-15

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Abstract

OBJECTIVE: To evaluate phytochemicals and in vitro biological potential of flowers, leaves and stem extracts of Rosa arvensis.

METHODS: Presence of twenty secondary metabolites was confirmed and then phenolic and flavonoid contents were quantified spectrophotometrically. Fourier Transform Infrared spectroscopy was conducted to ascertain functional groups and antioxidant potential was examined using 2,2-diphenyl-1-picrylhydrazyl scavenging activity, total antioxidant capacity and total reducing power assays. Human erythrocytes were used to assess anti-hemolytic activity and five bacterial strains were examined to determine antibacterial potential of plant extracts. Radish seeds were used to perform phytotoxic activity and cytotoxic potential was evaluated via brine shrimps and PC3 cell lines.

RESULTS: Highest phenolic contents were detected in the methanolic extract of Rosa arvensis flower (RAFM) [(151.635 ± 0.005) gallic acid equivalent mg/g] and highest flavonoid contents in the chloroform leaf extract (RALC) [(108.228 ± 0.004) quercetin equivalent mg/g]. Fourier-transform infrared spectroscopy analysis showed the presence of wide range of functional groups. The antioxidant assays indicated highest DPPH scavenging activity [IC50 (23.5 ± 0.6) μg/mL] in the methanolic stem extract (RASM), highest total antioxidant capacity [(265.1 ± 0.9) μg/mL] in RAFM and highest reducing potential [(209.9 ± 0.6) μg/mL] in leaf extract (RALM). Highest anti-hemolytic activity [(90.0 ± 0.5) μg/mL] was recorded in RAFM and brine shrimp cytotoxicity potential [(52.3 ± 0.3) μg/mL] in RASM. The antimicrobial activity was detected highest [(21.1 ± 0.5) mm inhibition zones] in RALM against Streptococcus aureus. In the end, anti-inflammatory and anti-cancer activity results depicted less than 50 % inhibition in the methanolic extracts.

CONCLUSIONS: Our findings will be helpful in designing pharmaceutical regimens and therefore, more studies can be recommended to isolate and characterize compounds associated with the biological activities of Rosa arvensis.

Key words： anti-inflammatory agents; spectroscopy, fourier transform infrared; hemolysis; oxidative stress; PC-3 cells

Cite this article

Tul Ain Zubaria , Fatima Iram , Naseer Sana , Kanwal Sobia , Mahmood Tariq . Assessment of phytochemicals, antioxidant, anti-hemolytic, anti-inflammatory and anti-cancer potential of flowers, leaves and stem extracts of Rosa arvensis[J]. Journal of Traditional Chinese Medicine, 2024 , 44(4) : 804 -812 . DOI: 10.19852/j.cnki.jtcm.2024.04.001

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