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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 4: 722 - 733

DOI: https://doi.org/10.19852/j.cnki.jtcm.20231226.001

Original articles

Effect on serum metabolomics of rats with premature ovarian insufficiency by Zhibian (BL54) through Shuidao (ST28) acupuncture

  • Jing YAN ,
  • Huimin FENG ,
  • Fang QIU ,
  • Haijun WANG ,
  • Luyun YIN ,
  • Xiaofei JIN ,
  • Jiyu ZHAO ,
  • Hongyang WANG ,
  • Xiaoqin YAN
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  • 1 Second Clinical College of Shanxi University of Traditional Chinese Medicine, Jinzhong 030619, China
    2 Research Center of Experimental Acupuncture Science, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
    3 Department of Cervical and Lumbar Vertebral Diseases, Jinzhong Hospital Affiliated to Shanxi University of Traditional Chinese Medicine, Jinzhong 030600, China

Received date: 2023-05-23

  Accepted date: 2023-09-12

  Online published: 2023-12-26

Supported by

Research Grant from the Natural Science Research Program of Shanxi Province: Based on Ultra-Performance Liquid Chromatography-Mass Spectrometry, the effect of "Zhibian (BL54) through Shuidao (ST28)" Needle Method on Ovarian Metabolism and Apoptosis Pathways in rats with Premature Ovarian Insufficiency was Discussed(2022030211216)

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Abstract

OBJECTIVE: To analyze the serum metabolic targets of the "Zhibian (BL54) through Shuidao (ST28)" acupuncture technique in cyclophosphamide (CTX)-induced premature ovarian insufficiency (POI) model rats and to elucidate the potential molecular mechanism of acupuncture in improving POI.

METHODS: We used an intraperitoneal injection of CTX to establish the POI rat model (POI group) and compared serum hormone levels and ovarian histopathological changes to evaluate the effect of the Zhibian (BL54) through Shuidao (ST28) technique (ZS + POI group) on ovarian function. Then, nontargeted metabolomics was performed using rat serum by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS).

RESULTS: After acupuncture intervention, the serum hormone levels and ovarian pathological morphology of POI rats were effectively improved. Moreover, UPLC-Q-TOF/MS results showed that the ZS + POI group showed a significant reversal of the levels of 6 differential metabolites. Among them, the levels of four serum metabolic markers, divanillyltetrahydrofuran ferulate, trans-ferulic acid, tryptamine, and neuraminic acid, increased significantly. Further analysis of biological effects showed that all metabolites were involved in the regulation of reproductive hormone levels and antioxidant and antiapoptotic effects.

CONCLUSIONS: The "Zhibian (BL54) through Shuidao (ST28)" acupuncture method may improve the ovarian function of POI rats by regulating serum metabolite markers to exert antioxidant and antiapoptotic effects, which provides a theoretical basis for the clinical application of acupuncture in the treatment of POI.

Key words： acupuncture; premature ovarian insufficiency; metabolomics; ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry; Zhibian (BL54) through Shuidao (ST28)

Cite this article

Jing YAN , Huimin FENG , Fang QIU , Haijun WANG , Luyun YIN , Xiaofei JIN , Jiyu ZHAO , Hongyang WANG , Xiaoqin YAN . Effect on serum metabolomics of rats with premature ovarian insufficiency by Zhibian (BL54) through Shuidao (ST28) acupuncture[J]. Journal of Traditional Chinese Medicine, 2024 , 44(4) : 722 -733 . DOI: 10.19852/j.cnki.jtcm.20231226.001

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