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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 2: 260 - 267

DOI: https://doi.org/10.19852/j.cnki.jtcm.20240203.003

Anti-inflammatory mechanism of the non-volatile ingredients originated from Guanghuoxiang (Pogostemonis Herba) based on high performance liquid chromatography-heated electron spray ionization-high resolution mass spectroscope and cell metabolomics

  • Wenguang JING ,
  • Xiaoyu LIN ,
  • Chu LI ,
  • Xiaoliang ZHAO ,
  • Xianlong CHENG ,
  • Penglong WANG ,
  • Feng WEI ,
  • Shuangcheng MA
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  • 1 Institute of Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 102629, China
    2 School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China
    3 Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment of Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China

Received date: 2023-04-22

  Accepted date: 2023-09-05

  Online published: 2024-02-03

Supported by

Institute of Chinese Medicine Discipline Construction Project of National Institutes for Food and Drug Control: Disciplinary Construction Program of Chinese Medicine Institute of NIFDC(1020050090116);Training Fund for Academic Leaders of NIFDC(2023X10);Program of State Drug Administraion-Key Laboratory of Quality Control of Chinese Medicinal Materials and Decoction Pieces(2022GSMPA-KL02)

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Abstract

OBJECTIVE: To explore the anti-inflammatory components and mechanism of the non-volatile ingredients of patchouli.

METHODS: High performance liquid chromatography-heated electron spray ionization-high resolution mass spectroscope (HPLC-HESI-HRMS) was used to analyze the chemical constituents of the non-volatile ingredients of patchouli. The anti-inflammatory activity of ingredients was evaluated using lipopolysaccharide (LPS) induced RAW264.7 cell inflammation model, and the anti-inflammatory mechanism was investigated using multivariate statistical analysis of cell metabolomics.

RESULTS: The non-volatile ingredients of patchouli were characterized by HPLC-HESI-HRMS, and 36 flavonoids and 18 other components were identified. These ingredients of patchouli not only had a good protective effect on the LPS-induced inflammation model of RAW264.7 cells, but also regulated the expression levels of arginine, L-leucine, cholesterol, fructose and sorbitol by down-regulating arginine metabolism, aminoacyl-tRNA biosynthesis, polyol/sorbitol pathway, so as to reduce inflammation and reduce cell damage.

CONCLUSION: The non-volatile ingredients of patchouli had good anti-inflammatory effect and exerted its curative effect by regulating endogenous metabolic pathway to reduce inflammatory response.

Key words： Pogostemon; non-volatile ingredients; anti-inflammatory; cell metabolomics

Cite this article

Wenguang JING , Xiaoyu LIN , Chu LI , Xiaoliang ZHAO , Xianlong CHENG , Penglong WANG , Feng WEI , Shuangcheng MA . Anti-inflammatory mechanism of the non-volatile ingredients originated from Guanghuoxiang (Pogostemonis Herba) based on high performance liquid chromatography-heated electron spray ionization-high resolution mass spectroscope and cell metabolomics[J]. Journal of Traditional Chinese Medicine, 2024 , 44(2) : 260 -267 . DOI: 10.19852/j.cnki.jtcm.20240203.003

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