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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 2: 303 - 314

DOI: https://doi.org/10.19852/j.cnki.jtcm.20230510.001

Yemazhui (Herba Eupatorii Lindleyani) ameliorates lipopolysaccharide-induced acute lung injury via modulation of the toll-like receptor 4/nuclear factor kappa-B/nod-like receptor family pyrin domain-containing 3 protein signaling pathway and intestinal flora in rats

  • Li REN ,
  • Yang HAI ,
  • Xue YANG ,
  • Xianqin LUO
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  • 1 Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of Metabolic Diseases, College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 400016, China
    2 College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China

Received date: 2022-11-11

  Accepted date: 2023-05-06

  Online published: 2023-05-10

Supported by

Natural Science Foundation Project of Chongqing Municipality: a Metabolome-based Study on the Protective Mechanism of Yemazhui (Herba Eupatorii Lindleyani) Sesquiterpene Lactones Against Acute Lung Injury(cstc2021jcyj-msxmX0365);Science and Technology Research Program of Chongqing Municipal Education Commission: a Cytokine Storm-based Study of the Protective Effect of Yemazhui (Herba Eupatorii Lindleyani) Extract Intervention on COVID-19 Lung Injury(KJZD-K202215101)

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Abstract

OBJECTIVE: To investigate the impact of Yemazhui (Herba Eupatorii Lindleyani, HEL) against lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore its underlying mechanism in vivo.

METHODS: The chemical constituents of HEL were analyzed by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry method. Then, HEL was found to suppress LPS-induced ALI in vivo. Six-week-old male Sprague-Dawley rats were randomly divided into 6 groups: control, LPS, Dexamethasone (Dex), HEL low dose 6 g/kg (HEL-L), HEL medium dose 18 g/kg (HEL-M) and HEL high dose 54 g/kg (HEL-H) groups. The model rats were intratracheally injected with 3 mg/kg LPS to establish an ALI model. Leukocyte counts, lung wet/dry weight ratio, as well as myeloperoxidase (MPO) activity were determined followed by the detection with hematoxylin and eosin staining, enzyme linked immunosorbent assay, quantitative real time polymerase chain reaction, western blotting, immunohistochemistry, and immunofluorescence. Besides, to explore the effect of HEL on ALI-mediated intestinal flora, we performed 16s rRNA sequencing analysis of intestinal contents.

RESULTS: HEL attenuated LPS-induced inflammation in lung tissue and intestinal flora disturbance. Mechanism study indicated that HEL suppressed the lung coefficient and wet/dry weight ratio of LPS-induced ALI in rats, inhibited leukocytes exudation and MPO activity, and improved the pathological injury of lung tissue. In addition, HEL reduced the expression of tumor necrosis factor-alpha, interleukin-1beta (IL-1β) and interleukin-6 (IL-6) in bronchoalveolar lavage fluid and serum, and inhibited nuclear displacement of nuclear factor kappa-B p65 (NF-κBp65). And 18 g/kg HEL also reduced the expression levels of toll-like receptor 4 (TLR4), myeloid differentiation factor 88, NF-κBp65, phosphorylated inhibitor kappa B alpha (phospho-IκBα), nod-like receptor family pyrin domain-containing 3 protein (NLRP3), IL-1β, and interleukin-18 (IL-18) in lung tissue, and regulated intestinal flora disturbance.

CONCLUSIONS: In summary, our findings revealed that HEL has a protective effect on LPS-induced ALI in rats, and its mechanism may be related to inhibiting TLR4/ NF-κB/NLRP3 signaling pathway and improving intestinal flora disturbance.

Key words： Yemazhui (Herba Eupatorii Lindleyani); acute lung injury; anti-inflammation; toll-like receptor 4; nuclear factor kappa-B; nod-like receptor family pyrin domain-containing 3 protein; signal transduction; gastrointestinal microbiome

Cite this article

Li REN , Yang HAI , Xue YANG , Xianqin LUO . Yemazhui (Herba Eupatorii Lindleyani) ameliorates lipopolysaccharide-induced acute lung injury via modulation of the toll-like receptor 4/nuclear factor kappa-B/nod-like receptor family pyrin domain-containing 3 protein signaling pathway and intestinal flora in rats[J]. Journal of Traditional Chinese Medicine, 2024 , 44(2) : 303 -314 . DOI: 10.19852/j.cnki.jtcm.20230510.001

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