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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 1: 27 - 34

DOI: https://doi.org/10.19852/j.cnki.jtcm.20231024.002

Original articles

B-cell lymphoma-2 phosphorylation at Ser70 site-related autophagy mediates puerarin-inhibited the apoptosis of MC3T3-E1 cells during osteoblastogenesis

  • Xi LI ,
  • Xiangquan LIN ,
  • Dongdong CHEN ,
  • Hui LIU
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  • 1 the Third Clinical Medical College, Fujian Medical University
    2 Department of Orthopedics, Fuzhou Second Hospital, Fuzhou 350007, China

Received date: 2022-09-14

  Accepted date: 2022-12-22

  Online published: 2023-10-24

Supported by

Fujian Provincial Natural Science Foundation Project: Exploration of Crisis Warning Values for Intraoperative Electrophysiological Monitoring in High-risk Cervical Spondylosis Patients on the Brink of Paralysis(2019J01548);Fujian Provincial Clinical Medical Research Center for First Aid and Rehabilitation in Orthopaedic Trauma: Study on the Treatment of Osteoporosis(2020Y2014)

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Abstract

OBJECTIVE: To explore the relationship between autophagy and apoptosis regulated by puerarin during osteoblastogenesis.

METHODS: In this study, the effects of puerarin on the autophagic activity and apoptosis level of osteoblast precursors (MC3T3-E1 cells) was observed. Subsequently, the roles of puerarin on B-cell lymphoma-2 (Bcl-2) phosphorylation at different sites in osteoblast precursors were observed. The effect of puerarin on the interaction between Bcl-2 and autophagy regulatory molecule or pro-apoptotic molecule was also investigated using Co-immunoprecipitation assays. In addition, the effect of puerarin on mitochondrial membrane potential of osteoblast precursors was also identified by mitochondrial membrane potential fluorescence probe assays.

RESULTS: Our results showed that puerarin can promote the autophagic activity and apoptosis level of MC3T3-E1 cells. In addition, puerarin promoted Bcl-2 phosphorylation at Ser70 site, and the dissociation of Bcl-2-Beclin1 complex. Moreover, puerarin could enhance the binding of Bcl-2-Bcl-2-Associated X (Bax) complex in MC3T3-E1 cells. Furthermore, puerarin increased the mitochondrial membrane potential of MC3T3-E1 cells.

CONCLUSIONS: Therefore, puerarin promotes Beclin1 into autophagy flux through Bcl-2 phosphorylation at Ser70, thereby enhancing autophagy of osteoblast precursors, which mediates its anti-apoptotic role during osteoblastogenesis. Furthermore, the dissociation of Bcl-2-Beclin1 complex is conducive to the binding of Bcl-2-Bax complex, which resists the apoptosis of osteoblast precursors viathe increased mitochondrial membrane potential.

Key words： puerarin; osteoblasts; autophagy; apoptosis; lymphoma, B-cell

Cite this article

Xi LI , Xiangquan LIN , Dongdong CHEN , Hui LIU . B-cell lymphoma-2 phosphorylation at Ser70 site-related autophagy mediates puerarin-inhibited the apoptosis of MC3T3-E1 cells during osteoblastogenesis[J]. Journal of Traditional Chinese Medicine, 2024 , 44(1) : 27 -34 . DOI: 10.19852/j.cnki.jtcm.20231024.002

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