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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 1: 188 - 196

DOI: https://doi.org/10.19852/j.cnki.jtcm.20231110.005

Original articles

Differences in vascular endothelial function and serum proteome between obese people with phlegm-dampness constitution and balanced constitution

  • Linghui ZHU ,
  • Ziwei SUN ,
  • Yuanyuan GUAN ,
  • Meiyi LIU ,
  • Yi ZHENG ,
  • Ruoxi YU ,
  • Qi WANG ,
  • Lingru LI
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  • 1 School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China
    2 National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing 100029, China

Received date: 2022-12-22

  Accepted date: 2023-06-15

  Online published: 2023-11-10

Supported by

General Program of National Natural Science of China: Qigui Slimming Prescription Upgrades the Activating Ability of the Phlegm-Dampness Constitution to Improve Obesity by Regulating the Thermogenesis Axis of Clostridium Enterica-Phytosphingosine-SphK/S1P-Ca2+ Cycle(82374308);National Key Research and Development Program: Development of Dietary Intervention Series for the Elderly with Decreased Functionality(2022YFC2010104);National Nonprofit Institute Research Grant for Institute of Basic Theory for Chinese Medicine, CACMS: Methylation of Gut Microbiota-Host DNA Reveals the Mechanism of Promoting Phlegm-Dampness Constitution to Prevent Metabolic Diseases(YZ-202151)

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Abstract

OBJECTIVE: To evaluate the extent of vascular endothelial dysfunction and preliminary identify serum protein biomarkers associated with obese individuals at risk for cardiovascular disease (CVD).

METHODS: Fifteen obese volunteers with the phlegm-dampness constitution or balanced constitution were recruited for this study respectively. The clinical baseline data was collected, and the vascular endothelial function was evaluated using the EndoPATTM. Blood samples were collected for the serum proteome analysis. The differences in the serum protein expression levels between the two groups were detected and the protein interaction network analysis, correlation analysis, receiver operating characteristic (ROC) curve analysis, and random forest model investigation were conducted.

RESULTS: There were no statistical differences found in the baseline data. For vascular endothelial function, the reactive hyperemia index (RHI) of the phlegm-dampness constitution obese group was significantly lower than that of the balanced constitution obese group (1.46 ± 0.30 vs 2.82 ± 0.78, P < 0.0001), indicating vascular endothelial dysfunction. There are 66 differentially expressed serum proteins between the two groups. apolipoprotein A2 (ApoA2), angiotensin-converting enzyme 2 (ACE-2), interleukin-33 (IL-33), and forkhead box P3 (FoxP3) showed significant differences and area under curve values of their ROC curves were greater than 0.7 and correlated significantly with RHI.

CONCLUSION: Vascular endothelial dysfunction was present in the phlegm-dampness constitution obese group. Thus, alterations in the expression levels of key serum proteins, including ApoA2, ACE-2, IL-33, and FoxP3 could serve as potential biomarkers in the obese population at risk of CVD.

Key words： obesity; phlegm-dampness constitution; vascular endothelial function; proteome; reactive hyperemia index

Cite this article

Linghui ZHU , Ziwei SUN , Yuanyuan GUAN , Meiyi LIU , Yi ZHENG , Ruoxi YU , Qi WANG , Lingru LI . Differences in vascular endothelial function and serum proteome between obese people with phlegm-dampness constitution and balanced constitution[J]. Journal of Traditional Chinese Medicine, 2024 , 44(1) : 188 -196 . DOI: 10.19852/j.cnki.jtcm.20231110.005

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