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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 6: 1081 - 1091

DOI: https://doi.org/10.19852/j.cnki.jtcm.20231018.002

Research Articles

Bornyl acetate extracted from Sharen (Fructus Amomi) inhibits proliferation, invasion and induces apoptosis by suppressing phosphatidylinositol-3-kinase/protein kinase B signaling in colorectal cancer

  • Xiaohua LI ,
  • Zhihang DUAN ,
  • Jianjun YUE ,
  • Yongyu ZHANG ,
  • Yihang LI ,
  • Shifang LIU ,
  • Qu NIE ,
  • Depo YANG ,
  • Lixia ZHANG
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  • 1 School of Traditional Dai-Thai Medicine, West Yunnan University of Apllied Science, Jinghong 666100, China
    2 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Jinghong 666100, China
    3 Yunnan Branch, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Yunnan Key Laboratory of Southern Medicine Utilization, Jinghong 666100, China

Received date: 2022-08-23

  Accepted date: 2022-11-15

  Online published: 2023-11-01

Supported by

Underground Ecological Planting Technology and Base Establishment of Sharen (Fructus Amomi) in the forest(2017YFC1701102);Characteristic Dai-Medicine Resource ERC of West Yunnan University of Apllied Science(2022KYPT0004);Study on the symbiotic system of Sharen(Fructus Amomi);weevil pollination and its "push-pull" pollination mechanism(82260736);Major Science and Technology Special Plan of Yunnan Province(202102AA100020);Scientific and Technological Talents and Platform Plan of Yunnan Province(202105AG070011)

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Abstract

OBJECTIVE: To investigate the antitumor effects of bornyl acetate (BA) isolated from Sharen (Fructus Amomi) in colorectal cancer (CRC) and the underlying mechanisms.
METHODS: SW480 and HT29 cells were treated with increasing doses of BA in order to determine its antitumor effects in vitro. Cell viability, colony formation, cell cycle, and apoptosis as well as migration and invasion were assessed using various assays. In addition, the in vivo antitumor effects of BA were assessed using a xenograft mouse model. We then assessed the mechanism of action of BA by conducting pathway activator-mediated rescue experiments and assessed the protein levels by Western blot analysis.
RESULTS: BA showed anti-CRC tumor activities in vitro by suppressing cell proliferation and colony formation, inducing apoptosis, blocking cell cycle, and inhibiting migration and invasion. These effects were mediated via suppression of the phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) pathway. In the tumor xenograft experiment, BA was found to repress tumor growth in vivo with low toxicity.
CONCLUSIONS: The results demonstrated that BA exerts antitumor effects by suppressing the PI3K/AKT pathway, with low toxicity. Thus, BA might be a potential novel therapeutic agent for CRC.

Key words： bornyl acetate; colorectal neoplasms; phosphatidylinositol 3-kinase; protein kinases B; signal transduction

Cite this article

Xiaohua LI , Zhihang DUAN , Jianjun YUE , Yongyu ZHANG , Yihang LI , Shifang LIU , Qu NIE , Depo YANG , Lixia ZHANG . Bornyl acetate extracted from Sharen (Fructus Amomi) inhibits proliferation, invasion and induces apoptosis by suppressing phosphatidylinositol-3-kinase/protein kinase B signaling in colorectal cancer[J]. Journal of Traditional Chinese Medicine, 2023 , 43(6) : 1081 -1091 . DOI: 10.19852/j.cnki.jtcm.20231018.002

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