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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 6: 1072 - 1080

DOI: https://doi.org/10.19852/j.cnki.jtcm.2023.06.005

Research Articles

Qiteng Xiaozhuo granules (芪藤消浊颗粒) medicated serum inhibits excessive proliferation and promotes apoptosis of human glomerular mesangial cells by targeting fat mass and obesity associated proteins

  • Xingxing ZHUANG ,
  • Tao LIU ,
  • Liangbing WEI ,
  • Junmei SONG ,
  • Jiarong GAO
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  • 1 Department of Pharmacy, the First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230012, China
    2 Department of Pharmacy, Chaohu Hospital of Anhui Medical University, Chaohu 238000, China
    3 School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230013, China
    4 Department of Pharmacy, the First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230012, Chin
    5 School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230013, China
    6 Department of Pharmacy, the First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230012, China
    7 School of Biological and Environmental Engineering, Chaohu University, Chaohu 238000, China
    8 Department of Pharmacy, the First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230012, China
    9 School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230013, China

Received date: 2022-09-23

  Accepted date: 2022-12-24

  Online published: 2023-09-01

Supported by

Lnc NONRATG001910.2 Competitively Binds miR-339 to Regulate the Mechanism of Syk Involvement in the Pathogenesis of Chronic Nephritis and the Intervention of Qiteng Xiaozhuo Particles(81973546);Study on the Mechanism of FTO-Mediated FOXO6 mRNA m6A Modification Regulating the Proliferation and Apoptosis of Glomerular Mesangial Cells(2022AH050747);Expression and Target Analysis of miR-155-5p in Kidney Tissue of CGN Rats(XLY-201912)

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Abstract

OBJECTIVE: To explore whether fat mass and obesity associated proteins (FTO) is an important target of Qiteng Xiaozhuo granules (QTXZG,芪藤消浊颗粒) medicated serum in regulating proliferation and apoptosis of glomerular mesangial cells.
METHODS: Medicated serum was obtained from Sprague-Dawley (SD) rats administered intragastrically with QTXZG decoction. The optimal concentration and intervention time of medicated serum were selected with the cell counting kit 8 assay. Cell proliferation was assessed by 5-ethynyl-2′-deoxyuridine (EdU) and cell apoptosis was investigated using flow cytometry. The expression of FTO, Proliferating cell nuclear antigen, Cyclin D1, B-cell lymphoma 2 (Bcl2) and BCL2 assaciated X was detected by Western blot and Real-time quantitative polymerase chain reaction, respectively. Quantification of the m6A RNA methylation was utilized to determine the total level of m6A methylation modification.
RESULTS: EdU and flow cytometry assays revealed that QTXZG medicated serum can remarkably inhibit proliferation and promote apoptosis of lipopolysaccharide (LPS)-induced human glomerular mesangial cells (HGMCs). The FTO overexpression plasmid could inhibit proliferation and promote apoptosis of LPS-induced HGMCs. The FTO inhibitor (FB23-2) can significantly attenuate the effect of QTZXG medicated serum on inhibiting excessive proliferation and promoting apoptosis. QTXZG medicated serum can significantly increase FTO expression and decrease the level of m6A methylation modification.
CONCLUSIONS: FTO is a key target for QTXZG medicated serum in inhibiting excessive proliferation and promoting apoptosis of human glomerular mesangial cells.

Key words： Qiteng Xiaozhuo granules; alpha-ketoglutarate-dependent dioxygenase FTO; medicated serum; cell proliferation; apoptosis

Cite this article

Xingxing ZHUANG , Tao LIU , Liangbing WEI , Junmei SONG , Jiarong GAO . Qiteng Xiaozhuo granules (芪藤消浊颗粒) medicated serum inhibits excessive proliferation and promotes apoptosis of human glomerular mesangial cells by targeting fat mass and obesity associated proteins[J]. Journal of Traditional Chinese Medicine, 2023 , 43(6) : 1072 -1080 . DOI: 10.19852/j.cnki.jtcm.2023.06.005

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