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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 5: 955 - 962

DOI: https://doi.org/10.19852/j.cnki.jtcm.20221111.002

Efficacy of electroacupuncture stimulating Zusanli (ST36) and Xuanzhong (GB39) on synovial angiogenesis in rats with adjuvant arthritis

  • Jianzhen JIANG ,
  • Xin ZHANG ,
  • Zhenguo LUO ,
  • Chengguo SU ,
  • Haiyan ZHOU ,
  • Yuqing JIANG ,
  • Xianjun XIAO ,
  • Yunfei CHEN ,
  • Jun ZHU
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  • 1 School of Acupuncture-Moxibustion and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China
    2 Vocational Training Department, Acupuncture and Moxibustion School Affiliated to Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China
    3 Department of Acupuncture, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China

Received date: 2022-04-12

  Accepted date: 2022-07-21

  Online published: 2022-11-11

Supported by

Natural Science Foundation-funded Project: Study of Electroacupuncture on the Angiogenesis in Rheumatoid Arthritis Rats by HIF-1α / MDM2 / p53 Signaling Pathway(81704152);Study on Immune Mechanisms of Macrophage M1/M2 Polarization in the Treatment of Rheumatoid Arthritis by Moxibustion “trengthening Body Resistance and Eliminating Evil”(81973959);Foundation of Science and Technology Department of Sichuan Province: Study of Electroacupuncture on the Punnus in Rheumatoid Arthritis Rats by HIF-1α，STAT3, Noth1 Signaling Pathway(2019YJ0491)

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Abstract

OBJECTIVE: To investigate the efficacy of electroacupuncture (EA) stimulating Zusanli (ST36) and Xuanzhong (GB39) on synovial angiogenesis in rats with adjuvant arthritis (AA).
METHODS: AA models were established by bilateral injection of Freund’s complete adjuvant (FCA) in male Sprague-Dawley rats. Three days after injection, rats were given EA at Zusanli (ST36) and Xuanzhong (GB39) acupoints, once every other day, for 16 d. The arthritis index score, paw volume, and hematoxylin-eosin (HE) staining was performed for each animal. Angiogenesis marker cluster of differentiation 34 (CD34) expression and synovial cell apoptosis in synovial tissue were observed. The levels of Notch1, hairy and enhancer of split homolog-1 (Hes1), transforming growth factor-beta (TGF-β) and basic fibroblast growth factor (bFGF) were subsequently detected.
RESULTS: We found that EA significantly decreased arthritis index scores, paw volume, and HE staining scores. EA could significantly inhibit the expression of CD34, promoting apoptosis of synovial cells in the joint synovial tissue of AA rats. The expression of Notch1 signaling pathway proteins and mRNAs (Notch1, Hes1, TGF-β, and bFGF) were markedly downregulated by EA treatment.
CONCLUSIONS: These results prove that EA attenuates synovial angiogenesis by inhibiting the Notch1 signaling pathway in AA rat models. Based on our findings, we propose that EA is a promising complementary and alternative therapy in rheumatoid arthritis.

Key words： electroacupuncture; arthritis, experimental; synovial angiogenesis; receptor, Notch1; signal transduction

Cite this article

Jianzhen JIANG , Xin ZHANG , Zhenguo LUO , Chengguo SU , Haiyan ZHOU , Yuqing JIANG , Xianjun XIAO , Yunfei CHEN , Jun ZHU . Efficacy of electroacupuncture stimulating Zusanli (ST36) and Xuanzhong (GB39) on synovial angiogenesis in rats with adjuvant arthritis[J]. Journal of Traditional Chinese Medicine, 2023 , 43(5) : 955 -962 . DOI: 10.19852/j.cnki.jtcm.20221111.002

References

1. Aletaha D, Smolen JS. Diagnosis and management of rheumatoid arthritis: a review. JAMA 2018; 320: 1360-72.
2. Veale DJ, Orr C, Fearon U. Cellular and molecular perspectives in rheumatoid arthritis. Semin Immunopathol 2017; 39: 343-54.
3. Elshabrawy HA, Chen Z, Volin MV, Ravella S, Virupannavar S, Shahrara S. The pathogenic role of angiogenesis in rheumatoid arthritis, Angiogenesis 2015; 18: 433-48.
4. Hao F, Wang Q, Liu L, et al. Effect of moxibustion on autophagy and the inflammatory response of synovial cells in rheumatoid arthritis model rat. J Tradit Chin Med 2022; 42: 73-82.
5. MacDonald IJ, Liu SC, Su CM, Wang YH, Tsai CH, Tang CH. Implications of angiogenesis involvement in arthritis. Int J Mol Sci 2018; 19: 2012.
6. Sun W, Zhang H, Wang H, et al. Targeting Notch-activated M1 macrophages attenuates joint tissue damage in a mouse model of inflammatory arthritis. J Bone Miner Res 2017; 32: 1469-80.
7. Jiao Z, Wang W, Guo M, et al. Expression analysis of Notch-related molecules in peripheral blood T helper cells of patients with rheumatoid arthritis. Scand J Rheumatol 2010; 39: 26-32.
8. Keewan E, Naser SA. Notch-1 signaling modulates macrophage polarization and immune defense against mycobacterium avium paratuberculosis infection in inflammatory diseases. Microorganisms 2020; 8: 1006.
9. An L, Li Z, Shi L, et al. Inflammation-targeted celastrol nanodrug attenuates collagen-induced arthritis through NF-κB and Notch1 pathways. Nano Lett 2020; 20: 7728-36.
10. Liu Q, Allen TD, Song W, Wada Y, Lobe CG, Liu J. Notch1 activates angiogenic regulator Netrin4 in endothelial cells. J Cell Mol Med 2019; 23: 3762-66.
11. Gao W, Sweeney C, Walsh C, et al. Notch signalling pathways mediate synovial angiogenesis in response to vascular endothelial growth factor and angiopoietin 2. Ann Rheum Dis 2013; 72: 1080-88.
12. Zeng C, Bai X, Qin H, Wang H, Rong X, Yan J. Effect of adjuvant therapy with electroacupuncture on bone turnover markers and interleukin 17 in patients with rheumatoid arthritis. J Tradit Chin Med 2019; 39: 582-6.
13. Zhou Y, Zhu J, Li LB, et al. Effects of electroacupuncture on joint function in rheumatoid arthritis patients of liver-and kidney-Yin deficiency type. Zhen Ci Yan Jiu 2016; 41: 440-6.
14. Ouyang BS, Gao J, Che JL, et al. Effect of electro-acupuncture on tumor necrosis factor-α and vascular endothelial growth factor in peripheral blood and joint synovia of patients with rheumatoid arthritis. Chin J Integr Med 2011; 17: 505-9.
15. Gusm?o JNFM, Fonseca KM, Ferreira BSP, et al. Electroacupuncture reduces inflammation but not bone loss on periodontitis in arthritic rats. Inflammation 2021; 44: 116-28.
16. Du ZH, Zhang CW, Xie WX, et al. Adenosine A2A receptor mediates inhibition of synovitis and osteoclastogenesis after electroacupuncture in rats with collagen-induced arthritis. Evid Based Complement Alternat Med 2019; 2019: 4617464.
17. Zhu J, Su CG, Chen YZ, Hao X, Jiang JZ. Electroacupuncture on ST36 and GB39 acupoints inhibits synovial angiogenesis via downregulating HIF-1α/VEGF expression in a rat model of adjuvant arthritis. Evid Based Complement Alternat Med 2019; 2019: 5741931.
18. Su CG, Chen YZ, Chen YF, et al. Effect of electroacupuncture at the ST36 and GB39 acupoints on apoptosis by regulating the p53 signaling pathway in adjuvant arthritis rats. Mol Med Rep 2019; 20: 4101-10.
19. Leech M, Metz C, Santos L, et al. Involvement of macrophage migration inhibitory factor in the evolution of rat adjuvant arthritis. Arthritis Rheum 1998; 41: 910-17.
20. Ierna M, Kerr A, Scales H, Berge K, Griinari M. Supplementation of diet with krill oil protects against experimental rheumatoid arthritis. BMC Musculoskelet Disord 2010; 11: 136.
21. Majchrzak K, Kaspera W, Szyma? J, Bobek-Billewicz B, Hebda A, Majchrzak H. Markers of angiogenesis (CD31, CD34, rCBV) and their prognostic value in low-grade gliomas. Neurol Neurochir Pol 2013; 47: 325-31.
22. Balogh E, Biniecka M, Fearon U, Veale DJ, Szekanecz Z. Angiogenesis in inflammatory arthritis. Isr Med Assoc J 2019; 5: 345-52.
23. Liu Z, Liu Y, Xu H, et al. Effect of electroacupuncture on urinary leakage among women with stress urinary incontinence: a randomized clinical trial. JAMA 2017; 317: 2493-501.
24. Maracle CX, Kucharzewska P, Helder B, et al. Targeting non-canonical nuclear factor-κB signalling attenuates neo-vascularization in a novel 3D model of rheumatoid arthritis synovial angiogenesis. Rheumatology (Oxford) 2017; 56: 294-302.
25. Nygaard G, Firestein GS. Restoring synovial homeostasis in rheumatoid arthritis by targeting fibroblast-like synoviocytes. Nat Rev Rheumatol 2020; 16: 316-33.
26. Zhi LQ, Zhong Q, Ma JB, Xiao L, Yao SX, Wang X. LncRNA H19 inhibitor represses synovial cell proliferation and apoptosis in rats with rheumatoid arthritis via Notch signaling pathway. Eur Rev Med Pharmacol Sci 2020; 24: 4088-94.
27. Kim MJ, Park JS, Lee SJ, et al. Notch1 targeting siRNA delivery nanoparticles for rheumatoid arthritis therapy. J Control Release 2015; 216: 140-8.
28. Li X, Dong Y, Yin H, Qi Z, Wang D, Ren S. Mesenchymal stem cells induced regulatory dendritic cells from hemopoietic progenitor cells through Notch pathway and TGF-β synergistically. Immunol Lett 2020; 222: 49-57.
29. Ma MJ, Liu HC, Qu XQ, Wang JL. Matrix metalloproteinase-3 gene polymorphism and its mRNA expression in rheumatoid arthritis. Genet Mol Res 2015; 14: 15652-59.
30. Gossage DL, Cieslarová B, Ap S, et al. Phase 1b study of the safety, pharmacokinetics, and disease-related outcomes of the matrix metalloproteinase-9 inhibitor andecaliximab in patients with rheumatoid arthritis. Clin Ther 2018; 40: 156-65.
31. Zou W, Chen QX, Sun XW, et al. Acupuncture inhibits Notch1 and Hes1 protein expression in the basal ganglia of rats with cerebral hemorrhage. Neural Regen Res. 2015; 10: 457-62.
32. Guo HD, Tian JX, Zhu J, et al. Electroacupuncture suppressed neuronal apoptosis and improved cognitive impairment in the AD model rats possibly via downregulation of Notch signaling pathway. Evid Based Complement Alternat Med 2015; 2015: 393569.
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