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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 4: 744 - 750

DOI: https://doi.org/10.19852/j.cnki.jtcm.20230524.002

Uncovering pharmacological mechanisms of Phellinus linteus on focal segmental glomeruloscleosis rats through tandem mass tag-based quantitative proteomic analysis, network pharmacology analysis and experimental validation

  • Feng WAN ,
  • Ruchun YANG ,
  • Yuewen TANG
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  • Department of Nephrology, Key Laboratory of Kidney Disease Prevention and Control Technology, Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou 310007, China

Received date: 2022-04-11

  Accepted date: 2022-08-15

  Online published: 2023-05-24

Supported by

Public Welfare Technology Application Research Program of Zhejiang Province: Study on the Application of Phellinus linteus Protocatechualdehyde in Inhibiting Glomerular Extracellular Matrix Accumulation based on TSP-1 Double Luciferase Reporting System(LGC21H290002);Key Projects of Zhejiang Administration of Traditional Chinese Medicine: Quality and Safety Evaluation of Cultivated Phellinus linteus in Zhejiang Province and its Comprehensive Application in the Prevention and Treatment of Kidney Disease(2020ZZ016)

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Abstract

OBJECTIVE: To explore the underlying molecular mechanism of Phellinus linteus (P. linteus).

METHODS: We used a tandem mass tag-based quantitative proteomic method to determine the differentially expressed proteins. Network pharmacology analysis was used to analysis the main components of P. linteus and construct the compound-target network. Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) were used to validate the analyses results.

RESULTS: The expression levels of thrombospondin-1 (TSP-1) and transforming growth factor (TGF)-β1/Smad3 signaling pathway proteins were significantly upregulated in focal segmental glomeruloscleosis (FSGS) rats. The P. linteus reduced the expression levels of TSP-1 and TGF-β1 signaling pathway proteins. Network pharmacology analysis revealed that protocatechualdehyde was the main active component. Subsequent in vivo and in vitro experiments validated the results of proteomic and network pharmacology analyses.

CONCLUSIONS: Our results suggested that P. linteus may inhibit renal sclerosis by inhibiting TSP-1-activated TGF-β1 signaling and may have potential applications in the treatment of FSGS.

Key words： Phellinus; glomerulosclerosis; focal segmental; thrombospondin 1; transforming growth factor beta1; smad3 protein

Cite this article

Feng WAN , Ruchun YANG , Yuewen TANG . Uncovering pharmacological mechanisms of Phellinus linteus on focal segmental glomeruloscleosis rats through tandem mass tag-based quantitative proteomic analysis, network pharmacology analysis and experimental validation[J]. Journal of Traditional Chinese Medicine, 2023 , 43(4) : 744 -750 . DOI: 10.19852/j.cnki.jtcm.20230524.002

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