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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 3: 484 - 493

DOI: https://doi.org/10.19852/j.cnki.jtcm.20230214.005

Protective effect of Tongdu Tiaoshen acupuncture combined with Xiaoxuming decoction (小续命汤) on dopaminergic neurons in Parkinson’s disease model

  • Haiyang WU ,
  • Ying WANG ,
  • Wei HAN ,
  • Huihui LI ,
  • Haisheng JI ,
  • Xiuxiu LIU
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  • 1 Department of encephalopathy, the Second Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230061, China
    2 Deparment of acupuncture and Moxibustion, The Affiliated Brain Hospital of Guangzhou Medical University, Guangzhou 510370, China
    3 Department of acupuncture and massage, Anhui University of Chinese Medicine, Hefei 230061, China

Received date: 2022-08-03

  Accepted date: 2022-11-23

  Online published: 2023-02-14

Supported by

Study on the Mechanism of Regulating miR-124 by Tongdu Tiaoshen Acupuncture to Promote Neuroprotection in Cerebral Ischemia Reperfusion Injury(81973933);Study on the Regulatory Mechanism of Tongdu Tiaoshen Acupuncture on Dopaminergic Neurons in Parkinson's Disease Model Mice Based on Mitochondrial Autophagy(KJ2019A0475)

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Abstract

OBJECTIVE: To explore the possible mechanism of Tongdu Tiaoshen acupuncture combined with Xiaoxuming decoction (小续命汤, XXMD) in the treatment of Parkinson’s disease (PD).

METHODS: C57BL/6 mice were randomly divided into eight groups (n = 12), including blank group, model group, medication group, acupuncture group, high-dose XXMD group (XXMD-H), low-dose XXMD group (XXMD-L), acupuncture combined with high-dose XXMD group (A+H), and acupuncture combined with low-dose XXMD group (A+L). After treatment for 6 weeks, dopamine (DA) neurons and the pathological changes of tyrosine hydroxylase (TH) positive cells were observed. The enzyme-linked immunosorbent assay (ELISA) was used to measure the content of DA and the level of interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10) and tumor necrosis factor alpha (TNF-α). The mRNA level of PINK1 and Parkin and the protein expression of Nix, PINK1 and Parkin in the substantia nigra were also detected.

RESULTS: Combination treatment effectively ameliorated the symptoms of PD. Compared with model group, combined treatment significantly up-regulated the protein expression of Nix, Parkin and PINK1 and the mRNA levels of PINK1 and Parkin in the substantia nigra (P<0.0001, P<0.001, P<0.01 or P<0.05). Furthermore, the levels of pro-inflammation cytokines were obviously decreased after combination therapy, while IL-10 content was increased remarkably (P<0.01).

CONCLUSION: Compared with each treatment alone, combination therapy improved the pathological damage of DA neurons of PD mice more effectively. The possible mechanism may be attributed to the up-regulated level of mitochondrial autophagy and improved mitochondrial function. These results provide fresh insight into the mechanism of co-treatment with Tongdu Tiaoshen acupuncture and XXMD for PD.

Key words： Parkinson disease; acupuncture; dopaminergic neurons; Tongdu Tiaoshen; Xiaoxuming decoction; mitochondrial autophagy

Cite this article

Haiyang WU , Ying WANG , Wei HAN , Huihui LI , Haisheng JI , Xiuxiu LIU . Protective effect of Tongdu Tiaoshen acupuncture combined with Xiaoxuming decoction (小续命汤) on dopaminergic neurons in Parkinson’s disease model[J]. Journal of Traditional Chinese Medicine, 2023 , 43(3) : 484 -493 . DOI: 10.19852/j.cnki.jtcm.20230214.005

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