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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 2: 295 - 302

DOI: https://doi.org/10.19852/j.cnki.jtcm.20221108.002

Original articles

Efficacy of Baishao Luoshi decoction (白芍络石方) on synaptic plasticity in rats with post stroke spasticity

  • Le XIE ,
  • Guo MAO ,
  • Yao XIE ,
  • Sijia CAO ,
  • Shen ZHOU ,
  • Junlin JIANG ,
  • Ting YAO ,
  • Jianhu FAN ,
  • Dong LIU ,
  • Fuliang KANG ,
  • Dahua WU ,
  • Jinwen GE
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  • 1 Neurology Department, Hunan Academy of Chinese Medicine, Changsha 410006, China
    2 School of Humanities and Management, Hunan University of Chinese Medicine, Changsha 410208, China
    3 College of pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550002, China
    4 College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha 410208, China

Received date: 2021-11-18

  Accepted date: 2022-02-16

  Online published: 2023-03-14

Supported by

China Postdoctoral Science Foundation: Research on the Mechanism of Baishaoluoshi Decoction in Improving Post-stroke Spasticity by KCC2-GABAAR Based on the Theory of “Gan Zhu Jin”(2020M682571);Natural Science Foundation of Hunan Province China: Research on the Mechanism of Post-stroke Spasticity by KCC2--GABAAR-Motoneuron Excitability and Chinese Traditional Medicine Intervention Based on the Theory of “Gan Zhu Jin”(2019JJ50343);Scientific Research Project of Education Department of Hunan Province: Research on the Mechanism of Axonal Regeneration and TCM Intervention in Post-Stroke Spasticity Based on the Theory of “Gan Zhu Jin” by Slit-Robo Pathway(19C1423)

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Abstract

OBJECTIVE: To evaluate the efficacy of Baishao Luoshi decoction (白芍络石方, BD) on synaptic plasticity in rats with post stroke spasticity (PSS), and to study the mechanism behind the action.

METHODS: The PSS model of rat was established by middle cerebral artery occlusion (MCAO). The neurological deficit symptoms were evaluated by modified neurological deficit score (mNSS). Muscle tension were evaluated by Modified Ashworth score (MAS). Transmission electron microscopy (TEM) was used to observe the synaptic ultrastructure. The expression of synaptic plasticity-related protein brain derived neurotrophic factor (BDNF), growth associated protein-43 (GAP43), synaptophysin (p38) and microtubule-associated protein 2 (MAP2) in the brain tissue around the infarct were detected by Western blotting.

RESULTS: We found that mNSS were significantly improved and limb spasticity was ameliorated treated by BD. The thickness of postsynaptic density and the synaptic curvature increased significantly. The expression of synaptic plasticity-related protein BDNF, GAP43, p38, MAP2 in the brain tissue around the infarct were raised remarkably after treated by BD.

CONCLUSIONS: Alleviating PSS by BD may be related to rescuing the synaptic plasticity, which provides a probable new therapeutic method for PSS.

Key words： infarction, middle cerebral artery; neuroprotection; post stroke spasticity; synaptic plasticity; Baishaoluoshi decoction

Cite this article

Le XIE , Guo MAO , Yao XIE , Sijia CAO , Shen ZHOU , Junlin JIANG , Ting YAO , Jianhu FAN , Dong LIU , Fuliang KANG , Dahua WU , Jinwen GE . Efficacy of Baishao Luoshi decoction (白芍络石方) on synaptic plasticity in rats with post stroke spasticity[J]. Journal of Traditional Chinese Medicine, 2023 , 43(2) : 295 -302 . DOI: 10.19852/j.cnki.jtcm.20221108.002

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