Journal of Traditional Chinese Medicine ›› 2023, Vol. 43 ›› Issue (2): 295-302.DOI: 10.19852/j.cnki.jtcm.20221108.002
• Original articles • Previous Articles Next Articles
XIE Le1, MAO Guo1, XIE Yao1, CAO Sijia2, ZHOU Shen1, JIANG Junlin1, YAO Ting1, FAN Jianhu1, LIU Dong3, KANG Fuliang1, WU Dahua1(), GE Jinwen1,4()
Received:
2021-11-18
Accepted:
2022-02-16
Online:
2023-04-15
Published:
2023-03-14
Contact:
Prof. WU Dahua, Neurology Department, Hunan Academy of Chinese Medicine Affiliated Hospital, Changsha 410006, China. 893049352@qq.com; Prof. GE Jinwen, College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha 410208, China 001267@hnucm.edu.cn. Telephone: +86-13508491768; +86-13873172948
Supported by:
XIE Le, MAO Guo, XIE Yao, CAO Sijia, ZHOU Shen, JIANG Junlin, YAO Ting, FAN Jianhu, LIU Dong, KANG Fuliang, WU Dahua, GE Jinwen. Efficacy of Baishao Luoshi decoction (白芍络石方) on synaptic plasticity in rats with post stroke spasticity[J]. Journal of Traditional Chinese Medicine, 2023, 43(2): 295-302.
Test item | Score |
---|---|
Motor function | |
Raising the rat by the tail: | |
Flexion of forelimb or hindlimb | 1 |
Head moved more than 10 ℃ to the vertical axis within 30 s | 1 |
Walking on the ground | |
Normal walking | 0 |
Circling toward the paretic side | 1 |
Fall down to the paretic side | 2 |
Abnormal movement | |
Immobility, staring, tremor and pilo-erection | 1 |
Myodystony and irritability | 1 |
Sensory function | |
Ipsilateral forelimb retracts after acupuncture | 0 |
Ipsilateral forelimb does not retract after acupuncture | 1 |
Reflex deficiency | |
Pinna reflex (head shake when touching the external auditory meatus) | 1 |
Corneal reflex (blink when lightly touching the cornea with cotton) | 1 |
Startle reflex (Motor response to sudden appearance of the noise) | 1 |
Total score | 11 |
Table 1 Modified neurological severity score
Test item | Score |
---|---|
Motor function | |
Raising the rat by the tail: | |
Flexion of forelimb or hindlimb | 1 |
Head moved more than 10 ℃ to the vertical axis within 30 s | 1 |
Walking on the ground | |
Normal walking | 0 |
Circling toward the paretic side | 1 |
Fall down to the paretic side | 2 |
Abnormal movement | |
Immobility, staring, tremor and pilo-erection | 1 |
Myodystony and irritability | 1 |
Sensory function | |
Ipsilateral forelimb retracts after acupuncture | 0 |
Ipsilateral forelimb does not retract after acupuncture | 1 |
Reflex deficiency | |
Pinna reflex (head shake when touching the external auditory meatus) | 1 |
Corneal reflex (blink when lightly touching the cornea with cotton) | 1 |
Startle reflex (Motor response to sudden appearance of the noise) | 1 |
Total score | 11 |
Figure 1 Chromatogram of BD established by HPLC A: reference sample of paeoniflorin, albiflorin and tracheloside, the main component of BD [Baishao (Baishao (Radix Paeoniae Alba) and Luoshiteng (Caulis Trachelospermi Jasminoidis) at a ratio of 1∶1]. Peaks 1-3 represented paeoniflorin, albiflorin and tracheloside respectively; B: HPLC chromatogram of three different batches of BD formula granules [Baishao (Radix Paeoniae Alba) (No. 16050101, 15090121, 16010069) and Luoshiteng (Caulis Trachelospermi Jasminoidis) (No. 15110003, 16082053, 16050059)]. BD: Baishaoluoshi decoction; HPLC: high performance liquid chromatography.
Figure 2 Neuroprotective effects of BD on PSS rats A: comparison of weight in each group at different time. Weight of rats in each group were detected at different time, 3 d after model established, 1, 2, 3, and 4 weeks after intervention; B: comparison of mNSS in each group at different time. mNSS was used to evaluate neurological deficits at different time, 3 d after model established, 1, 2, 3, and 4 weeks after intervention; C: comparison of MAS in each group at different time. MAS was used to evaluate spasticity of the affected fore limb 3 d after model established, 1, 2, 3, and 4 weeks after intervention. The PSS model was established by MCAO, and intervention began 3 d after model established for 4 weeks. Rats were daily treated orally with BD (10.8 g/kg in H and 5.4 g/kg in L) or baclofen (5.4 mg/kg in B), or normal saline (M, S, C) by gavage for 4 weeks. H: high dose BD group (10.8 g/kg); L: low dose BD group (5.4 g/kg); B: baclofen group (5.4 mg/kg); M: model group (normal saline); S: sham-operated group (normal saline); C: control group (normal saline). BD: Baishaoluoshi decoction; PSS: post stroke spasticity; mNSS: modified neurological deficit score; MAS: Modified Ashworth score; MCAO: middle cerebral artery occlusion. aP < 0.01 and bP < 0.05 as compared to model group (n = 12).
Figure 3 Effects of BD on the synaptic structure in PSS rats A: synaptic structure observed by TEM (×20 000). The red arrows represent synapse; B: comparison of PSD in the brain tissue around the infarcts; C: comparison of synaptic curvature in the brain tissue around the infarcts; D: comparison of synaptic gap in the brain tissue around the infarcts. Brain tissue around the infarct was stained by uranium acetate and lead citrate, and observed by TEM and the morphology of Gray 1 synapse was analyzed by image-pro Plus 6.0, 4 weeks after intervention. A1: high dose BD group (10.8 g/kg); A2: low dose BD group (5.4 g/kg); A3: baclofen group (5.4 mg/kg); A4: model group (normal saline); A5: sham-operated group (normal saline); A6: control group (normal saline). BD: Baishaoluoshi decoction; PSS: post stroke spasticity; TEM: transmission electron microscopy; PSD: postsynaptic density. bP < 0.05 and cP < 0.01 as compared to control group; dP < 0.05 and aP < 0.01 as compared to model group (n = 3).
Figure 4 Effects of BD on synaptic plasticity associated protein in PSS rats A: protein expression levels of BDNF, GAP43, p38 and MAP2 in brain tissue around the infarct were detected by Western blot analysis, 4 weeks after intervention. β-actin was used as the internal control. B: the relative expression of BDNF, C: the relative expression of GAP43, D: the relative expression of p38, E: the relative expression of MAP2: Densitometric analysis. H: high dose BD group (10.8 g/kg), L: low dose BD group (5.4 g/kg), B: baclofen group (5.4 mg/kg), M: model group (normal saline), S: sham-operated group (normal saline), and C: control group (normal saline). BD: Baishaoluoshi decoction; PSS: post stroke spasticity; BDNF: brain derived neurotrophic factor; GAP43: growth associated protein-43; MAP2: microtubule-associated protein 2. aP < 0.01, cP < 0.05, as compared to control group; bP < 0.01 and dP < 0.05, as compared to model group (n = 6).
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