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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 2: 274 - 285

DOI: https://doi.org/10.19852/j.cnki.jtcm.20230201.001

Original articles

Liver metabolomic characteristics in three different rat models of Yin deficiency based on ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry

  • Xingyao HU ,
  • Hongning LIU ,
  • Xiaojun YAN ,
  • Zhong CHEN ,
  • Liu FU ,
  • Ge LIU ,
  • Xuan CHEN ,
  • Guangbin SHANG
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  • Jiangxi University of Chinese Medicine Research Center for Differention and Development of Traditional Chinese Medicine (TCM) Basic Theory, Jiangxi Province Key Laboratory of TCM Etiopathogenisis, Nanchang 330004, China

Received date: 2022-07-10

  Accepted date: 2022-12-16

  Online published: 2023-03-14

Supported by

Jiangxi University of Chinese Medicine, Jiangxi Province Double First-Class Discipline of Traditional Chinese Medicine Construction Project(JXSYLXK-ZHYI026);Supported by Science and Technology Program of Jiangxi Provincial Health and Health Commission(SKJP220210415);Exploring the Formation Mechanism of Liver and Kidney Yin Deficiency Based on Animal Model and Metabolomics; Supported by National TCM Leading Talent Support Program of the State Administration of TCM-Qi Huang Scholar(284, China TCM people's teaching letter [2018])

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Abstract

OBJECTIVE: To investigate the mechanism of Yin deficiency syndrome (YDS) by analyzing the liver metabolomic characteristics of three different Yin deficiency rat models

METHODS: Following the TCM etiology, for clinical features and pathological manifestations of modern medicine, three kinds of animal models of Yin deficiency were induced and replicated. Totally 48 Sprague-Dawley (SD) male rats were randomly divided into blank group, irritation induced model group, Fuzi-Ganjiang induced model group, and thyroxine-reserpine induced model group. After successful development of model, the ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry was carried out to detect metabolites in each group. The metabolites of rat liver were analyzed for the characteristics of their biomarkers. The pathway enrichment analysis and metabolic network construction were performed through various online databases including Metabolite Biology Role, Human Metabolome Database, MetaboAnalyst, and Kyoto Encyclopedia of Genes and Genomes.

RESULTS: The SD rats in the experimental group showed symptoms like less weight gain, reduced diet and water intake, high body temperature, increased liver and kidney indexes, and abnormal liver and kidney tissue morphology. Moreover, the rats showed high increased levels of serum cyclic adenosine monophosphate, estradiol, alanine transaminase, and aspartate aminotransferase and decreased levels of cyclic guanosinc monophosphate and testosterone. We found four key interrelated metabolic pathways in the liver tissue metabolomics, including the biosynthesis of pantothenic acid and coenzyme A, and metabolism of alpha-linolenic acid metabolism, glycerophospholipid metabolism, and sphingolipid.

CONCLUSION: The liver and kidney YDS is closely related to the biosynthesis of pantothenic acid and CoA and abnormal metabolism of α-linolenic acid, glycerophospholipid, and sphingolipid in SD rats.

Key words： molecular biology; integrative medicine; Yin deficiency syndrome; syndrome complex

Cite this article

Xingyao HU , Hongning LIU , Xiaojun YAN , Zhong CHEN , Liu FU , Ge LIU , Xuan CHEN , Guangbin SHANG . Liver metabolomic characteristics in three different rat models of Yin deficiency based on ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry[J]. Journal of Traditional Chinese Medicine, 2023 , 43(2) : 274 -285 . DOI: 10.19852/j.cnki.jtcm.20230201.001

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