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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 1: 95 - 104

DOI: https://doi.org/10.19852/j.cnki.jtcm.20221017.001

Original articles

Mitochondrial dysfunction in a rat model and the related risk of metabolic disorders

  • Han LI ,
  • Xiaomin HUANG ,
  • Haiyang CAI ,
  • George HEROK ,
  • Jing HE ,
  • Yixun SU ,
  • Weihong LI ,
  • Chenju YI ,
  • Brian G OLIVER ,
  • Hui CHEN
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  • 1 Faculty of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
    2 Research Centre, the Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen 518107, China
    3 School of Life Sciences, University of Technology Sydney, Sydney, New South Wales 2059, Australia
Prof. LI Weihong, Faculty of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. liwihong@cdutcm.edu.cn. Telephone:+86-13419189905; +86-13882014290
Associate Prof. YI Chenju, Research Centre, the Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen 518107, China. yichj@mail.sysu.edu.cn;

Received date: 2021-09-16

  Accepted date: 2021-12-11

  Online published: 2023-01-10

Supported by

The Role of Mitochondrial Intergrity in the Pathophysiology of Kidney Yang Deficiency(81750110554);Central Mechanisms of Transgenerational Phenotype of Kidney Yang Deficiency in a Rat Model(2018HH0085);Molecular Mechanism of Blocking Cys259 and DD-mediated p75NTR Signaling Pathway to Delay the Progression of Alzheimer's Disease(NSFC 81971309);Mechanism Study of Cys259 Site and DD Domain of p75NTR as New Targets for the Treatment of Alzheimer's Disease(2019A1515011333);Research on Glial Connexin as a New Target for Alzheimer's Disease Treatment(F7201931620002)

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Abstract

OBJECTIVE: To explore whether kidney Yang deficiency (KYD) is prone to metabolic disorders may be linked to impaired mitochondrial function in thermogenesis and metabolic tissues.
METHODS: A rat model of KYD was used, which was established using Sprague Dawley rat dams with warm preference subjected to herbal treatment that can improve kidney Yang. The human relevance was confirmed by reduced serum corticosterone levels, and increased preference for warm location.
RESULTS: KYD Rats were underdeveloped. Adenosine-triphosphate (ATP) production was reduced in the brown fat, but increased in the muscle. However, oxidative phosphorylated complexes to generate ATP and mitochondrial biogenesis marker were reduced in both tissues. When the second insult of high-fat diet (HFD) was introduced, KYD rats gained less weight yet developed more severe lipid and glucose metabolic disorders. This may be driven by disregulated liver gluconeogenesis marker forkhead box protein O1 and lipid metabolic regulator cholesterol 7 alpha-hydroxylase.
CONCLUSION: KYD rats exhibited reduced mito-chondrial function in the brown fat, but were partially compensated by skeletal muscle, associated with the phenotype of warm preference and metabolic disorder, which was further exacerbated by additional HFD consumption. Future studies can focus on treatment targetting mitochondria function to reverse this phenotype.

Key words： kidney Yang deficiency; DNA, mitochondrial; adenosine triphosphate; thermogenesis; peroxisome proliferator-activated receptor gamma coactivator 1-alpha

Cite this article

Han LI , Xiaomin HUANG , Haiyang CAI , George HEROK , Jing HE , Yixun SU , Weihong LI , Chenju YI , Brian G OLIVER , Hui CHEN . Mitochondrial dysfunction in a rat model and the related risk of metabolic disorders[J]. Journal of Traditional Chinese Medicine, 2023 , 43(1) : 95 -104 . DOI: 10.19852/j.cnki.jtcm.20221017.001

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