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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 1: 87 - 94

DOI: https://doi.org/10.19852/j.cnki.jtcm.2023.01.008

Original articles

Astragaloside IV plays a role in reducing radiation-induced liver inflammation in mice by inhibiting thioredoxin-interacting protein/nod-like receptor protein 3 signaling pathway

  • Yanping DING ,
  • Xiaoqing DONG ,
  • Yifan MA ,
  • Lili CHEN ,
  • Jie ZHOU ,
  • Xinyan LI ,
  • Baoping SHAO
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  • 1 College of Life Science, Northwest Normal University, Lanzhou 730000, China
    2 College of Life Science, Lanzhou University, Lanzhou 730000, China
SHAO Baoping, College of Life Science, Lanzhou University, Lanzhou 730000, China. shaobp@lzu.edu.cn. Telephone:+86-931-7971414

Received date: 2021-12-11

  Accepted date: 2022-03-28

  Online published: 2023-01-10

Supported by

Molecular Mechanism of the Brain AQP4 Adaptation to the Extreme Hhypoxia in the Qinghai-Tibet Plateau Animal(31760271)

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Abstract

OBJECTIVE: To investigate the efficacy of Astragaloside IV (AS-IV) on radiation-induced liver inflammation in mice.
METHODS: The mice were divided into normal group, dimethyl sulfoxide solvent group, irradiation group (IR), irradiation + AS-IV (20 mg/kg) group (IR+AS-20) and irradiation + AS-IV (40 mg/kg) group (IR+AS-40). One month after intraperitoneal injection of AS-IV, the mice were irradiated with 8Gry Co60γ, the blood was collected for biochemical analysis, and the liver was collected for hematoxylin-eosin staining, immunofluorescence and electron microscopic observation, oxidative stress, and Western blot analysis.
RESULTS: The AS-IV treatment significantly ameliorated the pathological morphology of liver and reduced the alanine aminotransferase and aspertate amino-transferase levels in serum induced by radiation; AS-IV treatment also significantly reduced the expression of inflammatory factors tumor necrosis factor alpha and interleukin 6 and antagonized malonaldehyde content and superoxide dismutase activity in liver caused by radiation; in addition, AS-IV treatment can significantly inhibited the positive expression of thioredoxin-interacting protein (TXNIP) and nod-like receptor protein 3 (NLRP3) inflammasome in liver tissue after radiation; The expression of TXNIP, NLRP3 inflammasome, apoptosis-associated speck-like protein containing a CARD, cysteinyl aspartate-specific proteinase 1 and interleukin 1beta in the AS-IV prevention group decreased significantly compared to the radiation group.
CONCLUSIONS: These findings suggested that Co60γ radiation can cause structural and functional damage to the liver, which may be related to the NLRP3 mediated inflammatory pathway; AS-IV may play a protective role by inhibiting the TXNIP/NLRP3 inflammasome signaling pathway in the radiation-induced liver injury model.

Key words： radiation, ionizing; astragaloside IV; liver inflammation; NLR family, pyrin domain-containing 3 protein; thioredoxin-interacting protein

Cite this article

Yanping DING , Xiaoqing DONG , Yifan MA , Lili CHEN , Jie ZHOU , Xinyan LI , Baoping SHAO . Astragaloside IV plays a role in reducing radiation-induced liver inflammation in mice by inhibiting thioredoxin-interacting protein/nod-like receptor protein 3 signaling pathway[J]. Journal of Traditional Chinese Medicine, 2023 , 43(1) : 87 -94 . DOI: 10.19852/j.cnki.jtcm.2023.01.008

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