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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 6: 915 - 921

DOI: https://doi.org/10.19852/j.cnki.jtcm.20220922.001

Research Articles

Efficacy of Qingre Huashi decoction (清热化湿方) on infection of Helicobacter pylori: inhibiting adhesion, antioxidant, and anti-inflammation

  • Qiuyue HUANG ,
  • Hui YE ,
  • Zongming SHI ,
  • Xiaofen JIA ,
  • Miaomiao LIN ,
  • Yingming CHU ,
  • Jing YU ,
  • Xuezhi ZHANG
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  • Department of Endocrinology, People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou 350004, China
Prof. ZHANG Xuezhi, Department of Integrated Traditional Chinese and Western Medicine, Peking University First Hospital, Beijing 100034, China. zhang.xuezhi@263.net,Telephone: +86-10-83572634

Received date: 2021-12-12

  Accepted date: 2022-02-22

  Online published: 2022-09-22

Supported by

Research on Mechanisms of Jinghua Weikang Capsule Interving HP Infection and Related Inflammation through Adhersin-ROS/ MAPKR-NFκB Pathway(81973615);Effect and Mechanism of Jinghua Weikang Capsule Inhibiting the Adhesion Effect of Helicobacter pylori(7172220)

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Abstract

OBJECTIVE: To investigate the phytochemical profile of Qingre Huashi decoction (清热化湿方, QHD) and evaluate the mechanisms rationalizing the use of QHD against Helicobacter pylori (H. pylori)-associated gastritis.

METHODS: QHD is composed of 11 herbs, which was prepared by a fixed Pharmacy and concentrated into clear paste. High-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HPLC-QTOF/MS) was used to detect the phytochemical profile of QHD. The toxicity of QHD against H. pylori and human gastric epithelial cells was evaluated by the toxicology test and cell counting kit-8 assay. The adhesion model was constructed by incubating H. pylori and gastric mucosal epithelial cells for 2 h. The urease assay was used to examine the anti-adhesion effects of QHD, and gene expression of adhesins was evaluated by quantitative polymerase chain reaction. The antioxidant activity was assessed by DCFH-DA labeling. To evaluate the anti-inflammatory effect, the levels of pro-inflammatory cytokines in the culture supernatant were detected by enzyme-linked immunosorbent assay.

RESULTS: HPLC-QTOF/MS profiling indicated the presence of primary compounds 1-20 in QHD. Drug concentration was determined as 1, 2, and 5 mg/mL by the toxic concentration of QHD against H. pylori and human gastric epithelial cells. QHD prevented H. pylori adhesion to the human gastric epithelial cells and reduced levels of reactive oxygen species. QHD also reduced the level of interleukin-8 and other pro-inflammatory cytokines that were upregulated by H. pylori infection.

CONCLUSION: QHD could inhibit H. pylori adhesion, and exert antioxidant and anti-inflammatory effects in vitro.

Key words： Helicobacter pylori; bacterial adhesion; oxidative stress; inflammation; Qingre Huashi decoction

Cite this article

Qiuyue HUANG , Hui YE , Zongming SHI , Xiaofen JIA , Miaomiao LIN , Yingming CHU , Jing YU , Xuezhi ZHANG . Efficacy of Qingre Huashi decoction (清热化湿方) on infection of Helicobacter pylori: inhibiting adhesion, antioxidant, and anti-inflammation[J]. Journal of Traditional Chinese Medicine, 2022 , 42(6) : 915 -921 . DOI: 10.19852/j.cnki.jtcm.20220922.001

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