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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 5: 681 - 686

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.05.002

Research Articles

Efficacy of green tea extract on PC3 prostate cancer cells through upregulation of miR-195 expression and suppression of epithelial to mesenchymal transition

  • Safari Fatemeh ,
  • Dadeh Amirfard Katayoun
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  • 1 Department of Biology, Faculty of Science, University of Guilan, Rasht 4193833697, Iran
    2 Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran 1651153511, Iran
Fatemeh Safari, Department of Biology, Faculty of Science, University of Guilan, Rasht 4193833697, Iran.fsafari@guilan.ac.ir; Telephone: +98 1333333647

Received date: 2021-10-22

  Accepted date: 2022-01-29

  Online published: 2022-09-02

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Abstract

OBJECTIVE: To evaluate anticancer efficacy of green tea extract (GTE) on PC3 prostate cancer cells.

METHODS: By using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot methods, the expression of miR-195 and the epithelial to mesenchymal transition (EMT) markers such as E-cadherin and vimentin was analyzed.

RESULTS: Based on the results of 2D and 3D cell culture models, the inhibition of EMT and up regulation of miR-195 expression were detected.

CONCLUSIONS: Our findings will be helpful to design anti-tumor regimens with natural product original, and more studies will be required to identify the related mechanisms involving anticancer activities of green tea via miRNAs.

Key words： Green tea extract; PC3 cancer cells; epithelial-mesenchymal transition; miR-195 expression

Cite this article

Safari Fatemeh , Dadeh Amirfard Katayoun . Efficacy of green tea extract on PC3 prostate cancer cells through upregulation of miR-195 expression and suppression of epithelial to mesenchymal transition[J]. Journal of Traditional Chinese Medicine, 2022 , 42(5) : 681 -686 . DOI: 10.19852/j.cnki.jtcm.2022.05.002

References

1. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cáncer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA cancer J Clin 2018; 68: 394-424.
2. Trewartha D, Carter K. Advances in prostate cancer treatment. Nat Rev Drug Discov 2013; 12: 823-4.
3. de Craene B, Berx G. Regulatory networks defining EMT during cancer initiation and progression. Nat Rev Cancer 2013; 13: 97-110.
4. Jeong WS, Kim IW, Hu R, Kong AN. Modulatory properties of various natural chemopreventive agents on the activation of NF-kappaB signaling pathway. Pharm Res 2004; 21: 661-70.
5. Jigisha A, Nishant R, Navin K, Pankaj G. Green tea: a magical herb with miraculous outcomes. Int Res J Pharm 2012; 3: 139-48.
6. Cabrera C, Artacho R, Gimenez R. Beneficial effects of green tea-a review. J Am Coll Nutr 2006; 25: 79-99.
7. Lowe GM, Gana K, Rahman K. Dietary supplementation with green tea extract promotes enhanced human leukocyte activity. J Complement Integr Med 2015; 12: 277-82.
8. Boschmann M, Thielecke F. The effects of epigallocatechin-3-gallate on thermogenesis and fat oxidation in obese men: a pilot study. J Am Coll Nutr 2007; 26: 389S-95S.
9. Basu A, Sanchez K, Leyva MJ, et al. Green tea supplementation affects body weight, lipids, and lipid peroxidation in obese sub-jects with metabolic syndrome. J Am Coll Nutr 2010; 29: 31-40.
10. Ide K, Yamada H, Takuma N, et al. Green tea consumption affects cognitive dysfunction in the elderly: a pilot study. Nutrients 2014; 6, 4032-42.
11. Hwang JT, Ha J, Park IJ, et al. Apoptotic effect of EGCG in HT-29 colon cancer cells via AMPK signal pathway. Cancer Lett 2007; 247: 115-21.
12. Nakachi K, Matsuyama S, Miyake S, Suganuma M, Imai K. Preventive effects of drinking green tea on cancer and cardiovascular disease: epidemiological evidence for multiple targeting prevention. Biofactors 2000; 13: 49-54.
13. Shankar S, Ganapathy S, Hingorani SR, Srivastava RK. EGCG inhibits growth, invasion, angiogenesis and metastasis of pancreatic cancer. Front Biosci 2008; 13: 440-52.
14. Bartel DP. MicroRNAs: target recognition and regulatory functions. Cell 2009; 136: 215-33.
15. He L, Hannon G. MicroRNAs: small RNAs with a big role in gene regulation. Nat Rev Genet 2004; 5: 522-31.
16. Esquela-Kerscher A, Slack FJ. Oncomirs-microRNAs with a role in cancer. Nat Rev Cancer 2006; 6: 259-69.
17. Wu J, Ji A, Wang X, et al. MicroRNA-195-5p, a new regulator of Fra-1, suppresses the migration and invasion of prostate cancer cells. J Transl Med 2015; 13: 289.
18. Cai C, Chen QB, Han ZD, Zhang YQ, He HC, Chen JH, et al. miR-195 inhibits tumor progression by targeting RPS6KB1 in human prostate cancer. Clin Cancer Res 2015; 21: 4922-34.
19. Liang J, Li Y, Daniels G, et al. LEF1 targeting EMT in prostate Cancer invasion is regulated by miR-34a. Mol Cancer Res 2015; 13: 681-8.
20. Wang M, Ren D, Guo W, et al. Loss of miR-100 enhances migration, invasion, epithelial-mesenchymal transition and stemness properties in prostate cancer cells through targeting Argonaute 2. Int J Oncol 2014; 45: 362-72.
21. Wang H, Bian S, Yang CS. Green tea polyphenol EGCG suppresses lung cancer cell growth through upregulation miR-210 expression caused by stabilizing HIF-1a. Carcinogenesis 2011; 32: 1881-9.
22. Yamada S, Tsukamoto S, Huang Y, et al. Epigalocatechin-3-O-gallate up-regulates microRNA let-7b expression by activating 67-kDa laminin receptor signaling in melanoma cells. Sci Rep 2016; 6: 19225.
23. Mokhtari H, Yaghmaei B, Sirati-Sabet M, et al. Epigalocatechin-3-gallate enhances the efficacy of microRNA-34 mimic and microRNA-93 inhibitor co-transfection in prostate cancer cell line. Iran J Allergy Asthma Immuol 2020; 19: 612-23.
24. Lin CH, Wang HH, Chen TH, Chiang MC, Hung PH, Chen YJ. Involvement of MicroRNA-296 in the inhibitory e?ect of epigallocatechin gallate against the migratory properties of anoikis-resistant nasopharyngeal carcinoma cells. Cancers 2020; 12: 973.
25. Safari F, Rabieepor M, Jamalomidi F, Baghaeifar Z, Khodaei L. Evaluation of anti-cancer and pro-poptotic activities of iranian green tea extract (IGTE) against A549, PC3 and MCF-7 cancer cell lines. Int J Basic Sci Med 2019; 4: 113-8.
26. Rahmani Z, Safari F. Evaluating the in vitro therapeutic effects of human amniotic mesenchymal stromal cells on MiaPaca2 pancreatic cancer cells using 2D and 3D cell culture model. Tissue Cell 2021; 68: 101479.
27. Safari F, Shakery T, Sayadamin N. Evaluating the effect of secretome of human amniotic mesenchymal stromal cells on apoptosis induction and epithelial-mesenchymal transition inhibition in LNCaP prostate cancer cells based on 2D and 3D cell culture models. Cell Biochem Funct 2021; 39: 813-20.
28. Lamouille S, Xu J, Derynck R. Molecular mechanisms of epithelial-mesenchymal transition. Nat Rev Mol Cell Biol 2014; 15: 178-96.
29. Nijkamp MM, Span PN, Hoogsteen IJ, van der Kogel AJ, Kaanders JH, Bussink J. Expression of E-cadherin and vimentin correlates with metastasis formation in head and neck squamous cell carcinoma patients. Radiother Oncol 2011; 99: 344-8.
30. Lo UG, Lee CF, Lee MS, Hsieh JT. The role and mechanism of epithelial-to-mesenchymal transition in prostate cancer progression. Int J Mol Sci 2017; 18: 2079.
31. Yilmaz O, Sakaraya S. Is "Hanging Drop" a useful method to form spheroids of Jimt, Mcf-7, T-47d, Bt-474 that are breast cancer cell lines? Single Cell Biol 2018; 7: 1000170.
32. Chi KN, Agarwal N, Bjartell A, et al. Apalutamide for metastatic, castration-sensitive prostate cancer. NEJM 2019; 381: 13-24.
33. Davis ID, Martin AJ, Stockler MR, et al. Enzalutamide with standard first-line therapy in metastatic prostate cancer. NEJM 2019; 381: 121-31.
34. Guo J, Wang M, Liu X. MicroRNA-195 suppresses tumor cell proliferation and metastasis by directly targeting BCOX1 in prostate carcinoma. J Exp Clin Cancer Res 2015; 34:91.
35. Liu C, Guan H, Wang Y, et al. miR-195 inhibits EMT by targeting FGF 2 in prostate cancer cells. PLoS One 2015; 10: e0144073.
36. Cai C, He H, Duan X, et al. miR-195 inhibits cell proliferation and angiogenesis in human prostate cancer by downregulating PRR11 expression. Oncol Rep 2018; 39: 1658-70.
37. Bonuccelli G, Sotgia F, Lisanti MP. Matcha green tea (MGT) inhibits the propagation of cancer stem cells (CSCs), by targeting mitochondrial metabolism, glycolysis, and multiple cell signaling pathways. Aging 2018; 10: 1867-83
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