Journal of Traditional Chinese Medicine ›› 2022, Vol. 42 ›› Issue (5): 681-686.DOI: 10.19852/j.cnki.jtcm.2022.05.002
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Efficacy of green tea extract on PC3 prostate cancer cells through upregulation of miR-195 expression and suppression of epithelial to mesenchymal transition
Fatemeh Safari1(
), Katayoun Dadeh Amirfard2
- 1 Department of Biology, Faculty of Science, University of Guilan, Rasht 4193833697, Iran
2 Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran 1651153511, Iran
-
Received:2021-10-22Accepted:2022-01-29Online:2022-10-15Published:2022-09-02 -
Contact:Fatemeh Safari -
About author:Fatemeh Safari, Department of Biology, Faculty of Science, University of Guilan, Rasht 4193833697, Iran.fsafari@guilan.ac.ir; Telephone: +98 1333333647
Cite this article
Fatemeh Safari, Katayoun Dadeh Amirfard. Efficacy of green tea extract on PC3 prostate cancer cells through upregulation of miR-195 expression and suppression of epithelial to mesenchymal transition[J]. Journal of Traditional Chinese Medicine, 2022, 42(5): 681-686.
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Figure 1 Upregulation of miR-195 in PC3 cancer cells by GTE A: effect of GTE on PC3 cell viability in different concentration by using MTT assay; B: comparison of morphological changes of PC3 before and after treatment with GTE. B1: control; B2: treated. The experiments were performed three times (original microscope magnification, X 20, scale bar, 10 μm); C: relative expression of miR-195 in PC3 cancer cells during treatment with GTE was shown. Data represent mean ± standard deviation of three independent experiments. aP < 0.05 was considered to be statistically significant. GTE: green tea extract; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide
Figure 1 Upregulation of miR-195 in PC3 cancer cells by GTE A: effect of GTE on PC3 cell viability in different concentration by using MTT assay; B: comparison of morphological changes of PC3 before and after treatment with GTE. B1: control; B2: treated. The experiments were performed three times (original microscope magnification, X 20, scale bar, 10 μm); C: relative expression of miR-195 in PC3 cancer cells during treatment with GTE was shown. Data represent mean ± standard deviation of three independent experiments. aP < 0.05 was considered to be statistically significant. GTE: green tea extract; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide
Figure 2 Suppression of EMT by GTE in PC3 prostate cancer cells A: schematic model of EMT; B: effects of GTE on the migration of PC3 cells in different time (0 and 24 h after treatment). B1: control in 0 h after treatment; B2: control in 24 h after treatment; B3: treated in 0 h after treatment; B4: treated in 24 h after treatment. The images were obtained using phase-contrast microscopy. Scale bars represent 50 μm; C, D: relative expression of E-cadherin mRNA and relative expression of vimentin mRNA of PC3 cells after 48 h treatment with GTE were shown. Data represent mean ± standard deviation of three independent experiments. aP < 0.05 was considered to be statistically significant. E: by using Western blot, the expression of E-cadherin (precursor E-cadherin: upper band; mature E-cadherin: lower band; 135 and 120 kDa), and vimentin proteins (57 kDa) was shown and actin (42 kDa) used as an internal control. EMT: epithelial to mesenchymal transition; GTE: green tea extract; TCL: total cell lysate.
Figure 2 Suppression of EMT by GTE in PC3 prostate cancer cells A: schematic model of EMT; B: effects of GTE on the migration of PC3 cells in different time (0 and 24 h after treatment). B1: control in 0 h after treatment; B2: control in 24 h after treatment; B3: treated in 0 h after treatment; B4: treated in 24 h after treatment. The images were obtained using phase-contrast microscopy. Scale bars represent 50 μm; C, D: relative expression of E-cadherin mRNA and relative expression of vimentin mRNA of PC3 cells after 48 h treatment with GTE were shown. Data represent mean ± standard deviation of three independent experiments. aP < 0.05 was considered to be statistically significant. E: by using Western blot, the expression of E-cadherin (precursor E-cadherin: upper band; mature E-cadherin: lower band; 135 and 120 kDa), and vimentin proteins (57 kDa) was shown and actin (42 kDa) used as an internal control. EMT: epithelial to mesenchymal transition; GTE: green tea extract; TCL: total cell lysate.
Figure 3 Anti-tumor effect of GTE on PC3 cancer cells by using a 3D cell culture model A: schematic model of spheroids formation by using hanging drop technique; B, C: medium containing green tea inhibits size and number of spheroids (magnification: x20, Scale bar: 100 μm; Three independent experiments were done. aP < 0.05 was considered to be statistically significant); B1: control on day 3; B2: control on day 8; B3: treated on day 3; B4: treated on day 8. D-F: relative expression of E-cadherin, vimentin and miR-195 after treatment with GTE in PC3 cells (5 d) were shown. Data represent mean ± standard deviation of three independent experiments. aP < 0.05 was considered to be statistically significant. GTE: green tea extract.
Figure 3 Anti-tumor effect of GTE on PC3 cancer cells by using a 3D cell culture model A: schematic model of spheroids formation by using hanging drop technique; B, C: medium containing green tea inhibits size and number of spheroids (magnification: x20, Scale bar: 100 μm; Three independent experiments were done. aP < 0.05 was considered to be statistically significant); B1: control on day 3; B2: control on day 8; B3: treated on day 3; B4: treated on day 8. D-F: relative expression of E-cadherin, vimentin and miR-195 after treatment with GTE in PC3 cells (5 d) were shown. Data represent mean ± standard deviation of three independent experiments. aP < 0.05 was considered to be statistically significant. GTE: green tea extract.
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