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Journal of Traditional Chinese Medicine >

2012 , Vol. 32 >Issue 02: 222 - 228

DOI: https://doi.org/10.1016/s0254-6272(13)60015-1

Inhibition of hypoxia and serum deprivation-induced apoptosis by salvianolic acid in rat mesenchymal stem cells

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  • Guang'an men Hospital Affiliated to China Academy of Chinese Medical Sciences;Beijing University of Chinese Medicine;Zhejiang Provincial Xinhua Hospital;Fuwai Cardiovascular Hospital Affiliated to China Academy of Medical Sciences;

Accepted date: 2012-06-15

  Online published: 2012-06-15

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Abstract

OBJECTIVE:To investigate the influence and mechanism of salvianolic acid B(SalB) on apoptosis inhibition in rat bone marrow-derived mesenchymal stem cells(BMSCs) induced by hypoxia and serum deprivation(hypoxia/SD).METHODS:SalB concentration of 0.1,1,10 or 100 mg/L(drug groups) were investigated for their ability to inhibit apoptosis in rat BMSCs.BMSCs in both the apoptosis model and drug groups were cultured under hypoxic conditions for 6 h,after which cell apoptosis and change in mitochondrial membrane potential(MMP) were detected using flow cytometry.Activation of caspase-3 was detected using western blot analysis.RESULTS:Hypoxia/SD induced apoptosis in rat BMSCs.The early apoptosis rate was lower in the drug groups compared to the apoptosis model group(P

Key words： SalB, BMSCs, Hypoxia/SD, Apoptosis

Cite this article

Cao Wei , Guo Xiao Wen , Chen Kai , Xu Rui Xia , Zheng Hu Zhan , Wang Jie . Inhibition of hypoxia and serum deprivation-induced apoptosis by salvianolic acid in rat mesenchymal stem cells[J]. Journal of Traditional Chinese Medicine, 2012 , 32(02) : 222 -228 . DOI: 10.1016/s0254-6272(13)60015-1

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