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Journal of Traditional Chinese Medicine ›› 2012, Vol. 32 ›› Issue (02): 222-228.DOI: 10.1016/s0254-6272(13)60015-1

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Inhibition of hypoxia and serum deprivation-induced apoptosis by salvianolic acid in rat mesenchymal stem cells

Cao Wei , Guo Xiao Wen , Chen Kai , Xu Rui Xia , Zheng Hu Zhan , Wang Jie   

  1. Guang'an men Hospital Affiliated to China Academy of Chinese Medical Sciences;Beijing University of Chinese Medicine;Zhejiang Provincial Xinhua Hospital;Fuwai Cardiovascular Hospital Affiliated to China Academy of Medical Sciences;
  • Accepted:2012-06-15 Online:2012-06-15 Published:2012-06-15

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Abstract: OBJECTIVE:To investigate the influence and mechanism of salvianolic acid B(SalB) on apoptosis inhibition in rat bone marrow-derived mesenchymal stem cells(BMSCs) induced by hypoxia and serum deprivation(hypoxia/SD).METHODS:SalB concentration of 0.1,1,10 or 100 mg/L(drug groups) were investigated for their ability to inhibit apoptosis in rat BMSCs.BMSCs in both the apoptosis model and drug groups were cultured under hypoxic conditions for 6 h,after which cell apoptosis and change in mitochondrial membrane potential(MMP) were detected using flow cytometry.Activation of caspase-3 was detected using western blot analysis.RESULTS:Hypoxia/SD induced apoptosis in rat BMSCs.The early apoptosis rate was lower in the drug groups compared to the apoptosis model group(P

Key words: SalB, BMSCs, Hypoxia/SD, Apoptosis

Cite this article

Cao Wei , Guo Xiao Wen , Chen Kai , Xu Rui Xia , Zheng Hu Zhan , Wang Jie. Inhibition of hypoxia and serum deprivation-induced apoptosis by salvianolic acid in rat mesenchymal stem cells[J]. Journal of Traditional Chinese Medicine, 2012, 32(02): 222-228.