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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 3: 389 - 399

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.03.006

Research Article

Acupotomy inhibits aberrant formation of subchondral bone through regulating osteoprotegerin/receptor activator of nuclear factor-κB ligand pathway in rabbits with knee osteoarthritis induced by modified Videman method

  • Luxue QIN ,
  • Changqing GUO ,
  • Ruili ZHAO ,
  • Tong WANG ,
  • Junmei WANG ,
  • Yan GUO ,
  • Wei ZHANG ,
  • Tingyao HU ,
  • Xilin CHEN ,
  • Qian ZHANG ,
  • Dian ZHANG ,
  • Yue XU
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  • 1 School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China
    2 the First People's Hospital of Dongcheng District, Beijing 100050, China
    3 Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China
    4 the Third Affiliated Hospital of Beijing Universality of Chinese Medicine, Beijing 100029, China
Prof. GUO Changqing, School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China. Guochangqing66@163.com,Telephone: +86-10-64286687

Received date: 2021-10-22

  Accepted date: 2022-12-01

  Online published: 2022-05-20

Supported by

Beijing Municipal Natural Science Foundation: To Explore the Effect of Acupotomy on Subchondral Bone Remodeling in Early and Middle KOA Based on OPG/RANKL/RANK pathway(7192110)

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Abstract

OBJECTIVE: To investigate the effects of acupotomy on inhibiting abnormal formation of subchondral bone in rabbits with knee osteoarthritis (KOA).

METHODS: A total of 24 New Zealand rabbits were randomly divided into four groups of 6 rabbits each [control, model, electroacupuncture (EA) and acupotomy]. Eighteen KOA model rabbits were established using a modified Videman method. Rabbits in EA and acupotomy groups received the intervention for 3 weeks. Then, the cartilage and subchondral bone unit were obtained and the histomorphological changes were recorded. Osteo-protegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) in subchondral bone were evaluated by Western blotting, real-time polymerase chain reaction and immunohistochemistry.

RESULTS: Compared with the model group, both the acupotomy and EA groups showed a significant decrease in the Lequesne index (both P < 0.01) and Mankin score (P < 0.01, < 0.05). In addition, both EA and acupotomy groups had a higher expression of total articular cartilage (TAC) (P < 0.05, < 0.01) and lower expression of articular calcified cartilage (ACC)/TAC (P < 0.05, < 0.05) compared with the model group. The thickness of the subchondral bone plate in EA and acupotomy groups were decreased (both P < 0.01) compared to the model group. Moreover, trabecular bone volume (BV/TV), protein and relative expression of OPG and the ratio of OPG/RANKL in the subchondral bone of acupotomy group were decreased statistically significant, while these parameters were not significantly changed in the EA group compared with the model group.

CONCLUSIONS: In the rabbit model of KOA, acupotomy inhibits aberrant formation of subchondral bone by suppressing OPG/RANKL ratio as a potential therapy for KOA.

Key words： acupuncture therapy; osteogenesis; osteoarthritis,knee; subchondral bone

Cite this article

Luxue QIN , Changqing GUO , Ruili ZHAO , Tong WANG , Junmei WANG , Yan GUO , Wei ZHANG , Tingyao HU , Xilin CHEN , Qian ZHANG , Dian ZHANG , Yue XU . Acupotomy inhibits aberrant formation of subchondral bone through regulating osteoprotegerin/receptor activator of nuclear factor-κB ligand pathway in rabbits with knee osteoarthritis induced by modified Videman method[J]. Journal of Traditional Chinese Medicine, 2022 , 42(3) : 389 -399 . DOI: 10.19852/j.cnki.jtcm.2022.03.006

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