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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 2: 187 - 193

DOI: https://doi.org/10.19852/j.cnki.jtcm.20220218.001

Research Articles

Efficacy of aqueous extract of flower of Edgeworthia gardneri (Wall.) Meisn on glucose and lipid metabolism in KK/Upj-Ay/J mice

  • Chengfei ZHANG ,
  • Lingling QIN ,
  • Haiyan WANG ,
  • Boju SUN ,
  • Dan ZHAO ,
  • Qiue ZHANG ,
  • Fengying ZHONG ,
  • Lili WU ,
  • Tonghua LIU
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  • 1 School of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488, China
    2 Technology Department, Beijing University of Chinese Medicine, Beijing 100029, China
    3 Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100029, China
    4 Key Laboratory of TCM Health Cultivation of Beijing, Beijing University of Chinese Medicine, Beijing 102488, China
    5 First Clinical Medical College of Shaanxi University of Traditional Chinese Medicine, Shaanxi 712000, China
Correspondence to: Prof. LIU Tonghua, Key Laboratory of TCM Health Cultivation of Beijing, Beijing University of Chinese Medicine, Beijing 102488, China. thliu@vip.163.com, Telephone: +8613801020306

Received date: 2021-04-21

  Accepted date: 2021-07-28

  Online published: 2022-02-18

Supported by

Key Laboratory of TCM Health Cultivation of Beijing(BZ0259);Creation and Talent Introduction Base of Prevention and Treatment of Diabetes and Its Complications with Traditional Chinese Medicine(B20055)

Fold

Abstract

OBJECTIVE: To observe the effects of the flower of Edgeworthia gardneri (Wall.) Meisn (EWM) on glucose and lipid metabolism in KK/upj-Ay/J (KKAy) mice and investigate the possible mechanism of EWM in the liver of KKAy mice by transcriptome analysis.

METHODS: Forty KKAy mice were fed a high-sugar and high-fat diet for 3 weeks to establish the animal model of metabolic syndrome. After 5 weeks of continuous administration of EWM, serum high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFA) were detected by radioimmunoassay. Serum fasting insulin (Fins) and adiponectin levels were measured by enzyme-linked immunosorbent assay. Liver tissue fixed with paraformaldehyde was stained with hematoxylin-eosin and oil red O. Transcriptome analysis was used to evaluate the liver tissue. The expressions of lipoprotein lipase (LPL), peroxisome proliferator-activated receptor-γ (PPARγ), adenosine 5‘-monophosphate-activated protein kinase (AMPK), sterol regulatory element binding protein-1c (SREBP-1c), and fatty acid synthase (Fas) mRNA and protein in liver tissue were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis.

RESULTS: EWM slightly reduced FBG and Fins in KKAy mice. Furthermore, EWM was able to downregulate serum LDL, TG, TC, and FFA and upregulate the expression of serum HDL and adiponectin. Transcriptome analysis revealed the following differential pathways: the peroxisome proliferator-activated receptor (PPAR) signaling pathway and the AMPK signaling pathway. RT-PCR and western blot analysis detected the associated genes and proteins. In addition, EWM was able to upregulate the expression of AMPK and downregulate the expression of PPARγ, SREBP1c, and Fas mRNA and protein and upregulate the expression of LPL mRNA.

CONCLUSIONS: EWM can alleviate lipid metabolism disorders and to some extent improve glucose metabolism disorders in KKAy mice. These effects may be related to regulating PPARγ/LPL and activating the AMPK/SREBP1c/Fas pathway.

Key words： transcriptome; PPAR gamma; AMP-activated protein kinases; Edgeworthia gardneri (Wall.) Meisn flower

Cite this article

Chengfei ZHANG , Lingling QIN , Haiyan WANG , Boju SUN , Dan ZHAO , Qiue ZHANG , Fengying ZHONG , Lili WU , Tonghua LIU . Efficacy of aqueous extract of flower of Edgeworthia gardneri (Wall.) Meisn on glucose and lipid metabolism in KK/Upj-Ay/J mice[J]. Journal of Traditional Chinese Medicine, 2022 , 42(2) : 187 -193 . DOI: 10.19852/j.cnki.jtcm.20220218.001

References

1. Rader DJ. Effect of insulin resistance, dyslipidemia, and intra-abdominal adiposity on the development of cardiovascular disease and diabetes mellitus. Am J Med 2007; 120: S12-8.
2. Nolan PB, Carrick-Ranson G, Stinear JW, et al. Prevalence of metabolic syndrome and metabolic syndrome components in young adults: apooled analysis. Prev Med Rep 2017; 7: 211-5.
3. Tanaka N, Kimura T, Fujimori N, et al. Current status, problems, and perspectives of non-alcoholic fatty liver disease research. World J Gastroenterol 2019; 25: 163-77.
4. Younossi ZM, Koenig AB, Abdelatif D, et al. Global epidemiology of nonalcoholic fatty liver disease-Meta-analytic assessment of prevalence, incidence, and outcomes. Hepatology 2016; 64: 73-84.
5. Zhang Z, Xu H, Zhao H, et al. Edgeworthia gardneri (Wall.) Meisn. water extract improves diabetes and modulates gut microbiota. J Ethnopharmacol 2019; 239: 111854.
6. Gao D, Zhang YL, Yang FQ, et al. The flower of Edgeworthia gardneri (wall.) Meisn. suppresses adipogenesis through modulation of the AMPK pathway in 3T3-L1 adipocytes. J Ethnopharmacol 2016; 191: 379-86.
7. Wang H, Qin L, Wang D, et al. Mixture of five herbal extracts ameliorates pioglitazone-induced aggravation of hepatic steatosis via activating the adiponectin receptor 2/AMP-activated protein kinase signal pathway in diabetic KKAy mice. J Tradit Chin Med 2017; 37: 588-98.
8. Li Y, He PP, Zhang DW, et al. Lipoprotein lipase: from gene to atherosclerosis. Atherosclerosis 2014; 237: 597-608.
9. McCoy MG, Sun GS, Marchadier D, et al. Characterization of the lipolytic activity of endothelial lipase. J Lipid Res 2002; 43: 921-9.
10. Gross B, Pawlak M, Lefebvre P, Staels B. PPARs in obesity-induced T2DM, dyslipidaemia and NAFLD. Nat Rev Endocrinol 2017; 13: 36-49.
11. Hu S, Shen G, Zhao W, et al. Paeonol, the main active principles of Paeonia moutan, ameliorates alcoholic steatohepatitis in mice. J Ethnopharmacol 2010; 128: 100-6.
12. Dubois V, Eeckhoute J, Lefebvre P, et al. Distinct but complementary contributions of PPAR isotypes to energy homeostasis. J Clin Invest 2017; 127: 1202-14.
13. Ruderman NB, Carling D, Prentki M, et al. AMPK, insulin resistance, and the metabolic syndrome. J Clin Invest 2013; 123: 2764-72.
14. Yu X, McCorkle S, Wang M, et al. Leptinomimetic effects of the AMP kinase activator AICAR in leptin-resistant rats: prevention of diabetes and ectopic lipid deposition. Diabetologia 2004; 47: 2012-21.
15. Smith BK, Marcinko K, Desjardins EM, et al. Treatment of nonalcoholic fatty liver disease: role of AMPK. Am J Physiol Endocrinol Metab 2016; 311: E730-40.
16. Li Y, Xu S, Mihaylova MM, et al. AMPK phosphorylates and inhibits SREBP activity to attenuate hepatic steatosis and atherosclerosis in diet-induced insulin-resistant mice. Cell Metab 2011; 13: 376-88.
17. Sanders FW, Griffin JL. De novo lipogenesis in the liver in health and disease: more than just a shunting yard for glucose. Biol Rev Camb Philos Soc 2016; 91: 452-68.
18. Menendez JA, Vazquez-Martin A, Ortega FJ, et al. Fatty acid synthase: association with insulin resistance, type 2 diabetes, and cancer. Clin Chem 2009; 55: 425-38.
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