Advanced Search

Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 2: 167 - 175

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.02.002

Research Articles

Biosynthesis of titanium dioxide nanoparticles using Hypericum perforatum and Origanum vulgare extracts and their main components, hypericin and carvacrol as promising antibacterial agents

  • Khaksarian Mojtaba ,
  • Bahmani Mahmoud ,
  • Taherikalani Morovat ,
  • Ashrafi Behnam ,
  • Rafieian-Kopaei Mahmoud ,
  • Abbasi Naser
Expand
  • 1 Department of Physiology and Pharmacology, School of Medicine, Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
    2 Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran; Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
    3 Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
    4 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
    5 Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
    6 Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran; department of Pharmacology, Faculty of Medicine; Ilam University of Medical Sciences, Ilam, Iran
Dr. Mahmoud Bahmani; Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran. Bangjab Campus, Postal Code: 6939177143; mahmood.bahmani@gmail.com

Received date: 2020-10-28

  Accepted date: 2021-03-03

  Online published: 2022-04-01

Fold

Abstract

OBJECTIVE: To evaluate the anti-bacterial activities of titanium dioxide (TiO2) nanoparticles of Origanum (O.) vulgare and Hypericum (H.) perforatum extracts, carvacrol and hypericin against Staphylococcus (S.) aureus.

METHODS: In this study, TiO2 nanoparticles of O. vulgare and H. perforatum extracts, carvacrol and hypericin, were prepared and their antibacterial effects were evaluated against Staphylococcus (S.) aureus. In this study, scanning electron microscope, fourier transform infrared spectrometer, atomic force microscopy, dynamic light scattering and zeta potential were used to investigate the structure of synthesized drugs.

RESULTS: Anti-bacterial activity of synthesized NPs was tested by minimum inhibitory concentration (MIC), minimum bactericidal concentration and disc diffusion method. MICs of TiO2-NPs synthesized using O. vulgare, H. perforatum, carvacrol and hypericin and TiO2 were obtained 250, 62.5, 250, and 250, and 500 μg/mL, respectively. The MBCs for all of these were obtained 1000 μg/mL.

CONCLUSION: Green-synthesized of TiO2 nanoparticles provides a promising approach to the use of O. vulgare and H. perforatum, carvacrol and hypericin as novel agents and safer antibacterial compounds, especially anti-S. aureus compounds.

Key words： plant, medicinal; medicine, traditional; anti-infective agents; active ingredient; nano-compounds

Cite this article

Khaksarian Mojtaba , Bahmani Mahmoud , Taherikalani Morovat , Ashrafi Behnam , Rafieian-Kopaei Mahmoud , Abbasi Naser . Biosynthesis of titanium dioxide nanoparticles using Hypericum perforatum and Origanum vulgare extracts and their main components, hypericin and carvacrol as promising antibacterial agents[J]. Journal of Traditional Chinese Medicine, 2022 , 42(2) : 167 -175 . DOI: 10.19852/j.cnki.jtcm.2022.02.002

References

1. Kilic A, Basustaoglu AC. Double triplex real-time PCR assay for simultaneous detection of Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis, and Staphylococcus haemolyticus and determination of their methicillin resistance directly from positive blood culture bottles. Res Microbiol 2011; 162: 1060-6.
2. Jadhav S, Hussain A, Devi S, et al. Virulence characteristics and genetic affinities of multiple drug resistant uropathogenic escherichia coli from a semi urban locality in India. PLoS One 2011; 6: 18063.
3. El Farran CA, Sekar A, Balakrishnan A, Shanmugam S, Arumugam P, Gopalswamy J. Prevalence of biofilm- producing Staphylococcus epidermidis in the healthy skin of individuals in Tamil Nadu, India. Indian J Med Microbiol 2013; 31: 19-23.
4. Weichhart T, Horky M. Functional selection of vaccine candidate peptides from staphylococcus aureus whole-genome expression libraries in vitro. Infect immune 2003; 71: 4633-41.
5. Li W. Antibacterial activity and mechanism of silver nanoparticleson Escherichia coli. Appl Microbiol Biotechnol 2010; 2: 23-7.
6. Tenover FC. Mechanisms of antimicrobial resistance in bacteria. Am J Infect Control 2006; 34: 3-10.
7. Santhoshkumar T, Abdul Rahuman A, Jayaseelan CH, et al. Green synthesis of titanium dioxide nanoparticles using Psidium guajava extract and its antibacterial and antioxidant properties. Asian Pac J Trop Med 2014; 968-76.
8. Sawhney APS, Condon B. Modern applications of nanotechnology in textiles. Textile Research J 2008; 78: 731-9.
9. Docters T, Chovelon JM, Herrmann JM. Syntheses of TiO2 photocatalysts by the molten salts method application to the photocatalytic degradation of prosulfuron. Applied Catalysis B: Environmenta 2004; 150: 219-26.
10. Heinlaan M, Ivask A, Blinova I, Dubourguier HC, Kahru A. Toxicity of nanosized and bulk ZnO, CuO and TiO2 to bacteria vibrio fischeri and crustaceans Daphnia magna and Thamnocephalus platyurus. Chemosphere 2007; 71: 1308-16.
11. Zhu Y, Eaton JW, Li C. Titanium dioxide nanoparticles preferentially induce cell death in transformed cells in a Bak/Bax- independent fashion. PLoS One 2012; 7: 50607.
12. Aitken RJ, Chaudhry MQ, Boxall ABA, Hull M. Manufacture and use of nanomaterials: current status in the UK and global trends. Occupa Med 2006; 56: 300-6.
13. Gao Y, Musoda Y, Seo WS, Ohta H, Koumoto K. TiO2 nanoparticles prepared using an aqueous peroxotitanate solution. Ceramics Intern 2004; 30: 1365-8.
14. Lettieri S, Pavone M, Fioravanti A, Santamaria Amato L, Maddalena P. Charge carrier processes and optical properties in TiO2 and TiO2-based heterojunction photocatalysts: a review. Materials (Basel, Switzerland) 2021; 14: 1645.
15. Boujday S, Wunsch F, Portes P, Bocquet JF, Justin CC. Solar energy mater. Solar Cells 2004; 83: 421.
16. Carp O, Huisman CL, Reller A. Photoinduced reactivity of titanium dioxide. Prog Solid Chem 2004; 32: 33-177.
17. Ruiz AM, Sakai G, Cornet A, Shimanoe K, Morante RJ, Yamazoe. Sens N. Insights into the structural and chemical modifications of Nb additive on TiO2 nanoparticles. Actuators B: Chem 2004; 103: 312.
18. Zhao J, Bowman L, Zhang X, et al. Titanium dioxide (TiO2) nanoparticles induce JB6 cell apoptosis through activation of the caspase-8/Bid and mitochondrial pathways. J Toxicol Environ Health A 2009; 72: 1141-9.
19. Sundrarajan M, Gowri S. Green synthesis of titanium dioxide nanoparticles by nyctathes arbor tristis leaves extract. Chalcogenide Letters 2011; 8: 447-51.
20. Selvakesavan RK, Franklin G. Prospective application of nanoparticles green synthesized using medicinal plant extracts as novel nanomedicines. Nanotechnol Sci Applic 2021; 14: 179-95.
21. Bahmani M, Taherikalani M, Khaksarian M, Soroush S, Ashrafi B, Heydari R. Phytochemical profiles and antibacterial activities of hydroalcoholic extracts of origanum vulgare and hypericum perforatum and carvacrol and hypericin as a promising anti-staphylococcus aureus. Mini-Rev Med Chem 2019; 19: 923-32.
22. Bahmani M, Taherikalani M, Khaksarian M, et al. The synergistic effect of hydroalcoholic extracts of Origanum vulgare, Hypericum perforatum and their active components carvacrol and hypericin against Staphylococcus aureus. Future Sci OA 2019; 5: Article number FSO371.
23. Abbasi N, Khosravi A, Aidy A, Shafiei M. Biphasic response to luteolin in MG-63 osteoblast-like cells under high glucose-induced oxidative stress. Iranian J Med Sci 2016; 41: 118-25.
24. Sankar R, Dhivya R, Subramanian Shivashangari K, Ravikumar V. Wound healing activity of Origanum vulgare engineered titanium dioxide nanoparticles in Wistar Albino rats. J Mater Sci Mater Med 2014; 25: 1701-8.
25. Clinical and Laboratory Standards Institute (CLSI). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: Approved standard, eighth ed. (Document M7-A8). 2009; CLSI. Wayne.
26. Nostro A, Sudano Roccaro A, Bisignano G, et al. Effects of oregano, carvacrol and thymol on Staphylococcus aureus and Staphylococcus epidermidis biofilms. J Med Microbiol 2007; 56: 519-23.
27. Dadgar T, Ghaemi E, Asmar M, Mazandarani M, Bazueri M. Antibacterial activities of six medicinal plants against methicillin-resistant and sensitive Staphylococcus aureus. Iranian J Med Arom Plants 2007; 23: 73-85.
28. García I, Ballesta S, Gilaberte Y, Rezusta A, Pascual A. Antimicrobial photodynamic activity of hypericin against methicillin-susceptible and resistant Staphylococcus aureus biofilms. Future Microbiol 2015; 10: 347-56.
29. Kashef N, Karami S, Djavid GE. Phototoxic effect of hypericin alone and in combination with acetylcysteine on Staphylococcus aureus biofilms. Photodiagnosis Photodyn Ther 2015; 12: 186-92.
30. Foster AH, Sheel WD, Sheel P, et al. Antimicrobial activity of titania/silver and titania/copper films prepared by CVD. J Photochem Photobiol A 2010; 216: 283-9.
31. Huck-Pezzei VA, Pallua JD, Pezzei G, et al. Fourier transform infrared imaging analysis in discrimination studies of St. John's wort (Hypericum perforatum). Anal Bioanal Chem 2012; 404: 1771-8.
32. Galehassadi M, Rezaii E, Najavand S, Mahkam M, Mohammadzadeh Gheshlaghi N. Isolation of carvacol from Origanum vulgare, synthesis of some organosilicon derivatives, and investigating of its antioxidant, antibacterial activities. Standard Sci Res Essays 2014; 2: 438-50.
33. Enyashin AN, Seifert G. Structure, stability and electronic properties of TiO2 nanostructures. Physica Status Solidi (b) 2005; 242: 1361-70.
34. Aitken RJ, Chaudhry MQ, Boxall ABA, Hull M. Manufacture and use of nanomaterials: current status in the UK and global trends. Occupa Med 2006; 56: 300-6.
35. Hu T. Anti-bacterial study using nano silver doped high density polyethylene pipe. Sustain Environ Res 2012; 22: 153-8.
36. Dutta RK, Nenavathu BP, Gangishetty MK, Reddy AV. Studies on antibacterial activity of zno nanoparticles by ros induced lipid peroxidation. Colloids Surf B Biointerfaces 2012; 94: 143-50.
37. Stoimenov PK, Klinger RL, Marchin GL, Klabunde KL. Metal oxide nanoparticles as bactericidal agents. Langmuir 2002; 18: 6679-86.
38. Badireddy AR, Hotze EM, Chellam S, Alvarez P, Wiesner MR. Inactivation of bacteriophages via photosensitization of fullerol nanoparticles. Environ Sci Technol 2007; 41: 6627-32.
39. Kikuchi Y, Sunada K, Iyoda T, Hashimoto K, Fujishima A. Photocatalytic bactericidal effect of TiO2 thin films: dynamic view of the active oxygen species responsible for the effect. J Photochem Photobiol A: Chem 1997; 106: 51-6.
40. Suttiponparnit K, Jiang J, Sahu M, Suvachittanont S, Charinpanitkul T, Biswas P. Role of surface area, primary particle size, and crystal phase on titanium dioxide nanoparticle dispersion properties. Nanoscale Res Lett 2011; 6: 27.
41. Raut RW, Kolekar NS, Lakkakula JR, Mendhulkar VD, Kashid SB. Extracellular of silver nanoparticles using dried leaves of Pongamia pinnata (L) Pierre. Nano-Micro Lett 2010; 2: 106-13.
42. Barzegary F, Jave A, Rezaei Zarch S. Antimicrobial activities of TiO2 Nanoparticle Against Escherichia coli and Staphylococcus aureus. J shahis sadoughi Uni Med Sci 2010; 18: 39-46.
43. Saadatmand MM, Yazdanshenas ME, Rezaei-Zarchi S, Yousefi-Telori B, Negahdary M. Investigation of anti-microbial properties of chitosan-TiO2 Nanocomposite and its use on sterile gauze pads. J Lab Sci 2012; 6: 59-72.
44. Aashis, Roy S, Parveen A, Anil R, Koppalkar MVN, Prasad A. Effect of Nano-titanium dioxide with different antibiotics against methicillin-resistant Staphylococcus Aureus. J Biomat Nanobiotechnol 2010: 1: 37-41.
45. Shahsavari S. An overview of the most important medicinal plants used in Iranian traditional medicine for the treatment of kidney stones: a mini-review article. Plant Biotechnol Persa 2021; 3: 1: 10.52547/pbp.3.1.4
46. Fattepur S, Nilugal KC, Darshan TT, et al. Toxicological and pharmacological activity of ethanolic extracts of Catharanthus roseus in expermental animals. Int J Med Toxicol Legal Med 2018; 21: 141-4.
47. Sengar M, Saxena S, Satsangee S, Jain R. Silver nanoparticles decorated functionalized multiwalled carbon nanotubes modified screen printed sensor for the voltammetric determination of butorphanol. J Appl Organometallic Chem 2021; 1: 95-108.
48. Giorgadze TG, Khutsishvili IG, Melikishvili ZG, Bregadze VG. Silver atoms encapsulated in G4 PAMAM (polyamidoamine) dendrimers as a model for their use in nanomedicine for phototherapy. Euro Chem Bull 2020; 9: 22-7.
49. Alwan S, Al-Saeed M, Abid H. Safety assessment and biochemical evaluation of biogenic silver nanoparticles (using bark extract of C. zeylanicum) in Rattus norvegicus rats: safety of biofabricated AgNPs (using Cinnamomum zeylanicum extract). Baghdad J Biochem Appl Biolog Sci 2021; 2: 138-50.
50. Simeon J, Thrush J, Bailey TA. Angiopoietin-like protein 4 is a chromatin-bound protein that enhances mammosphere formation in vitro and experimental triple-negative breast cancer brain and liver metastases in vivo. J Carcinog 2021; 20: 8.
51. Bhale SP, Yadav AR, Pathare PG, et al. Synthesis, characterization and antimicrobial activity of transition metal complexes of 4-[(2-Hydroxy-4-Methoxyphenyl) Methy-leneamino]-2, 4-Dihydro-3h-1, 2, 4-Triazole-3-Thione. Europ Chem Bull 2020; 9: 430-5.
52. Ma D, Han T, Karimian M, Abbasi N, Ghaneialvar H, Zangeneh A. Immobilized Ag NPs on chitosan-biguanidine coated magnetic nanoparticles for synthesis of propargylamines and treatment of human lung cancer. Int J Biolog Macromol 2020; 165: 767-75.
53. Wan AE, Khan MSB, Teo BSX, et al. Screening of antioxidant and antibacterial activity of methanolic extract of Ipomoea aquatica leaf and stem against bacteria causes skin infection. Int J Med Toxicol Legal Med 2020; 2: 169-78.
54. Teo BSX, Gan RY, Abdul Aziz S, et al. In vitro evaluation of antioxidant and antibacterial activities of Eucheuma cottonii extract and its in vivo evaluation of the wound‐healing activity in mice. J Cosm Dermatol 2021; 20: 993-1001.
55. Palaksha MN, Ahmed M, Das S. Antibacterial activity of garlic extract on streptomycin-resistant Staphylococcus aureus and Escherichia coli solely and in synergism with streptomycin. J Nat Sci Biol Med 2010; 1: 12-5.
56. Zharif N, Santosh F, Kiran CN, Fadli A, Ibrahim A, Nizam G. Synergistic effect of ethanolic extract of melastoma malabataricum leaves and antibiotics. Int J Med Toxicol Leg Med 2018; 21: 167.
57. Karimi E, Abbasi S, Abbasi N. Thymol polymeric nanoparticle synthesis and its effects on the toxicity of high glucose on OEC cells: involvement of growth factors and integrin-linked kinase. Drug Design, Development and Therapy 2019; 13: 2513-32.
58. Zhang Y, Mahdavi B, Mohammadhosseini M, et al. Green synthesis of NiO nanoparticles using Calendula officinalis extract: chemical charactrization, antioxidant, cytotoxicity, and anti-esophageal carcinoma properties. Arabian Journal of Chemistry 20121; 14: 103105.
59. Nurin RQ, Halim S. Potential pharmacotherapy of olea europaea (Olive) fruit oil against methamphetamine-induced neurotoxicity. Journal of Cellular and Molecular Anesthesia 2019; 4: 67-8.
60. Khalep HRH, Abidin AZ, Hassan H, Fattepur S, Othman Z. The anti-angiogenic properties of Morinda Citrifolia L. (Mengkudu) leaves using chicken chorioallantoic membrane (CAM) assay. Pharmacognosy Journal 2019; 11: 12-5.
61. Sathi SS, Kiran CN, Santosh F, Fadli A, May F, Ibrahim A, Jiyauddin K. Comparison of wound healing activity of piper betle and ocimum sanctum in wistar rats. International Journal of Medical Toxicology and Legal Medicine 2020; 23: 109-16.
62. Kaleemullah M, Jiyauddin K, Thiban E, Rasha S, Al-Dhalli S, Budiasih., Gamal OE, Fadli A, Eddy Y. Development and evaluation of Ketoprofen sustained release matrix tablet using Hibiscus rosa-sinensis leaves mucilage. Saudi Pharmaceutical J 2017; 25: 770-9.
Options
Outlines

/

〈 〉