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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 1: 9 - 16

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.01.001

Research Articles

In-vitro and in-vivo pharmacological screening of Iris albicans

  • Kamal Dawood ,
  • Roohullah ,
  • Rabbi Fazle ,
  • Naz Attiqa ,
  • Bilal Muhammad
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  • Department of Pharmacy, Abasyn University Peshawar, Peshawar, 25000, Khyber Pakhtunkhwa, Pakistan
Prof. Dr. Roohullah, Department of Pharmacy, Abasyn University Peshawar, Peshawar, 25000, Khyber Pakhtunkhwa, Pakistan. dr.roohullah2012@gmail.com

Received date: 2021-03-05

  Accepted date: 2021-08-20

  Online published: 2022-01-25

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Abstract

OBJECTIVE: To evaluate the anti-oxidant, enzyme inhibition, anti-pyretic, anti-inflammatory and anti-diabetic activities of Iris albicans.

METHODS: Anti-oxidant assay was evaluated using DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging and ABTS (2, 2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) inhibitory protocol while enzyme inhibitory assay was evaluated by lipoxygenase and cyclooxygenase-2 inhibitory protocol respectively. Anti-pyretic, anti-inflammatory and anti-diabetic potential was evaluated using brewer’s yeast induced pyrexia, carrageenan induced paw edema and streptozocin induced diabetes protocols respectively. Serum biochemical parameters were monitored for the period of study.

RESULTS: The anti-oxidant activity of chloroform fraction of Iris albicans showed the highest scavenging potential against DPPH and ABTS while the maximum inhibitory action recorded against lipo-oxygenase and cyclo-oxygenase-2 enzymes was shown by n-hexane and chloroform fractions respectively. The anti-pyretic potential of the crude methanolic extract showed dose dependent activity in reducing pyrexia, thereby when the dose was increased the anti-pyretic effect was also enhanced. The anti-inflammatory action of the crude methanolic extract administered at the dose of 300 mg/kg was significant at 1 h after its administration, which was found maintained up to 5 h. Similarly the anti-diabetic effect of the crude methanolic extract administered at the dose of 200 and 300 mg/kg was noted highly significant at day 6 and was found well maintained throughout the study time period up to 10 days. Significant (P < 0.001) improvement appeared in hemoglobin, protein, cholesterol, triglycerides, urea, creatinine, HDL and LDL of extract treated diabetic mice.

CONCLUSION: From this data it could be concluded that Iris albicans have significant anti-oxidant, enzyme inhibition, ant-pyretic, anti-inflammatory and anti-diabetic potential.

Key words： antioxidants; anti-inflammatory agent; antipyretics; hypoglycemic agents; Iris Albicans

Cite this article

Kamal Dawood , Roohullah , Rabbi Fazle , Naz Attiqa , Bilal Muhammad . In-vitro and in-vivo pharmacological screening of Iris albicans[J]. Journal of Traditional Chinese Medicine, 2022 , 42(1) : 9 -16 . DOI: 10.19852/j.cnki.jtcm.2022.01.001

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