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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 1: 49 - 57

DOI: https://doi.org/10.19852/j.cnki.jtcm.20210707.002

Research Articles

Therapeutic effects of salidroside vs pyrrolidine dithiocarbamate against severe acute pancreatitis in rats

  • Jing QIAN ,
  • Xiaohong WANG ,
  • Benzhong WEI ,
  • Guoxiong ZHOU ,
  • Shunxing ZHU ,
  • Chun LIU
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  • 1 Department of General Surgery, Yizheng Hospital of Nanjing Drum Tower Hospital Group, Yizheng 211900, China
    2 Department of Gastroenterology, Yizheng Hospital of Nanjing Drum Tower Hospital Group, Yizheng 211900, China
    3 Department of Anesthesiology, Yizheng Hospital of Nanjing Drum Tower Hospital Group, Yizheng 211900, China
    4 Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, China
    5 Department of Laboratory Animal Center, Nantong University, Nantong 226001, China
Dr. WANG Xiaohong, Department of Gastroenterology, Yizheng Hospital of Nanjing Drum Tower Hospital Group, Yizheng 211900, China. wx102474wx@163.com

Received date: 2021-01-09

  Accepted date: 2021-04-03

  Online published: 2021-07-07

Supported by

Traditional Chinese Medicine Technology Development Plan Project of Jiangsu Province in 2020: Basic and Clinical Study on Salidroside in the Adjuvant Treatment of Severe Acute Pancreatitis(YB2020088);Health Innovation Project of Lvyang Jinfeng Plan of Yangzhou City in 2020: Basic and Clinical Study on Salidroside in the Adjuvant Treatment of Severe Acute Pancreatitis(LJ202037)

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Abstract

OBJECTIVE: To evaluate the therapeutic effectiveness of salidroside (Sal) and pyrrolidine dithiocarbamate (PDTC) against severe acute pancreatitis (SAP) in a rat model.

METHODS: Rat models of SAP were established by retrograde infusion of sodium taurocholate solution. SAP rats were randomly divided into 6 groups: SAP 3 h group, SAP 24 h group, low-dose Sal treatment group (Sal L+S), middle-dose Sal treatment group (Sal M+S), high-dose Sal treatment group (Sal H+S) and PDTC treatment group (PDTC+S). The serum amylase, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-10 (IL-10) levels were determined by optical turbidimetry and enzyme-linked immunosorbent assay. The expression of Beclin-1, microtubule-associated protein light chain 3Ⅱ(LC3Ⅱ), lysosome associated membrane protein 2 (LAMP2), interleukin-1 receptor associated kinase 1 (IRAK1),inhibitor α of nuclear transcription factor-κB (IκBα), nuclear transcription factor-κB 65 (p65) in the pancreas tissues were detected by quantitative real-time polymerase chain reaction and Western blot, while the p-IκBα and p-p65 levels were detected by Western blot. Pathological changes of the pancreas and all the other indexes were observed at 3 and 24 h after operation.

RESULTS: The serum IL-10 level, IκBα and LAMP2 levels in Sal M+S, Sal H+S and PDTC+S groups were higher than those in SAP 24 h group, while all the other indexes in these three groups were all lower significantly than those in SAP 24 h group. There was no significant difference in all indexes between Sal H+S and PDTC+S groups.

CONCLUSION: High-dose Sal has an effectively therapeutic effect on SAP in rats, which was similar to PDTC.

Key words： rhodioloside; pyrrolidines; ethylenebis (dithio-carbamates); pancreatitis; autophagy; NF-kappa B; signal transduction

Cite this article

Jing QIAN , Xiaohong WANG , Benzhong WEI , Guoxiong ZHOU , Shunxing ZHU , Chun LIU . Therapeutic effects of salidroside vs pyrrolidine dithiocarbamate against severe acute pancreatitis in rats[J]. Journal of Traditional Chinese Medicine, 2022 , 42(1) : 49 -57 . DOI: 10.19852/j.cnki.jtcm.20210707.002

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