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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 1: 39 - 48

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.01.004

Research Articles

Shenweifang-containing serum inhibits transforming growth factor-β1-induced myofibroblast differentiation in normal rat kidney interstitial fibroblast cells

  • Jiaru LIN ,
  • Li WANG ,
  • Bo CHEN ,
  • Santao OU ,
  • Jianhua QIN ,
  • Junming FAN
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  • 1 Department of Nephrology, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China
    2 Nephropathy Clinical Medical Research Center of Sichuan Province, Luzhou 646000, China
    3 Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China
    4 Central Laboratory, Affiliated Chinese Medicine Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China
    5 Southwest Medical University, Luzhou 646000, China
    6 Chengdu Medical College, Chengdu, Sichuan 610500, China
FAN Junming, Southwest Medical University, Luzhou 646000, China; Department of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China; Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China; Central Laboratory, Affiliated Chinese Medicine Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China; Chengdu Medical College, Chengdu, Sichuan 610500, China. junmingfan@163.com; mumuxiaoru@126.com

Received date: 2020-09-21

  Accepted date: 2021-02-16

  Online published: 2022-01-25

Supported by

Department of Science and Technology of Southwest Medical University(2017-ZRQN-072);Department of Science and Technology of Affiliated Hospital of Southwest Medical University(16231)

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Abstract

OBJECTIVE: To investigate the efficacy of Shenweifang (SWF)-containing serum on transforming growth factor (TGF)-β1-induced fibroblast-myofibroblast transition in normal rat kidney interstitial fibroblast cells (NRK-49F).

METHODS: Sprague-Dawley rats were gavaged with one of five solutions: (a) saline; (b) saline plus low-dose SWF; (c) saline plus medium-dose SWF; (d) saline plus high-dose SWF; and (e) saline plus valsartan. NRK-49F cells were treated with TGF-β1 and cultured using serum from the gavaged rats.

RESULTS: TGF-β1 treatment increased the expression of α-smooth muscle actin, proliferating cell nuclear antigen, collagenⅠ, Smad3, mitogen-activated protein kinase (MAPK) 10, and c-Jun N-terminal kinase (JNK) 3 and induced abnormalities in cell morphology, cell cycle progression, and cell proliferation.

CONCLUSIONS: SWF- or valsartan-containing serum corrected (or partially corrected) TGF-β1-induced abnormal changes in this in vitro system. SWF-containing serum reversed abnormalities in morphology, cell cycle progression, and proliferation in TGF-β1-treated NRK-49F cells, probably by blocking the TGF-β1/Smads and TGF-β1/MAPK/JNK pathways.

Key words： fibrosis; transforming growth factor beta1; Smad proteins; mitogen-activated protein kinases; JNK mitogen-activated protein; kinases drug-containing serum; Shenweifang

Cite this article

Jiaru LIN , Li WANG , Bo CHEN , Santao OU , Jianhua QIN , Junming FAN . Shenweifang-containing serum inhibits transforming growth factor-β1-induced myofibroblast differentiation in normal rat kidney interstitial fibroblast cells[J]. Journal of Traditional Chinese Medicine, 2022 , 42(1) : 39 -48 . DOI: 10.19852/j.cnki.jtcm.2022.01.004

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