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Journal of Traditional Chinese Medicine ›› 2017, Vol. 37 ›› Issue (05): 659-666.

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Effect of electroacupuncture on expression of Ang/Tie-2 mRNA and protein in rats with acute cerebral infarction

Li Jing, Bai Zonglu, Du Yuanhao, Li Yongfeng, Zhang Xuezhu, Pang Bo, Zhang Jingjing   

  1. Department of Acupuncture and Moxibustion, the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine;Tianjin University of Traditional Chinese Medicine;Shanxi University of Chinese Medicine;
  • Accepted:2017-10-15 Online:2017-10-15 Published:2022-04-04
  • Supported by:
    Supported by Tianjin Natural Science Foundation of Key Projects:Acupuncture Adjustment Th17/Treg Balance Against Nerve Inflammation Damage(No.16JCZDJC36200), And the National Natural Science Foundation of China:Acupuncture Regulation VD Brain Nerve Inflammation and Peripheral Immune Suppression of the Effect and Mechanism(No.81473766), Based on ACE/ACE2 axis and VEGF-Dll4/Notch Pathway to Study the Molecular Mechanism of Acupuncture Intervention in the Establishment of Collateral Circulation of Cerebral Infarction(No.81674056), Molecular Mechanism of Neurotoxicity Induced by Acupuncture in Rats with Cerebral Infarction based on Shh and Wnt Signaling Pathway(No.81473765)

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Abstract: OBJECTIVE: To identify the intervention mechanism of the effect of electroacupuncture on the expression of Ang/Tie-2 m RNA and protein in rats with acute cerebral infarction induced by middle cerebral artery occlusion(MCAO).METHODS: Altogether 120 Wistar rats were subjected to MCAO by inserting a nylon filament, andthen divided into 3 groups: control group, injured group and electroacupuncture group. The injured and electroacupuncture groups were further divided into the following 7 subgroups according to the time after MCAO: 3, 6, 12, 24 h, 3, 7 and 12 d, with 8 rats in each subgroup. The electroacupuncture group was given electroacupuncture treatment at Shuigou(GV 26) instantly after operation. The rats were killed at different time points according to their groups, and then the expression levels of Ang/Tie-2 m RNA and protein were detected using Real-Time polymerase chain reaction and immunohistochemical staining.RESULTS: The m RNA and protein expression levels of Ang/Tie-2 in the electroacupuncture group were significant higher than that in the injured group.CONCLUSION: The results suggested that electroacupuncture could significantly regulate the expression of Ang/Tie-2 m RNA and protein in the rats with acute cerebral infarction induced by MCAO, and enhance angiogenesis after ischemic penumbra.

Key words: Infarction,middle cerebral artery, Electroacupuncture, Point GV26(Shuigou), Angiopoietins, Receptor,TIE-2, Angiogenesis

Cite this article

Li Jing, Bai Zonglu, Du Yuanhao, Li Yongfeng, Zhang Xuezhu, Pang Bo, Zhang Jingjing. Effect of electroacupuncture on expression of Ang/Tie-2 mRNA and protein in rats with acute cerebral infarction[J]. Journal of Traditional Chinese Medicine, 2017, 37(05): 659-666.