Efficacy of Qiancao (Radix Rubiae Cordifoliae) and Haipiaoxiao (Endoconcha Sepiellae), a traditional Chinese herb pair, on abnormal uterine bleeding in rats with incomplete abortion

doi:10.19852/j.cnki.jtcm.20220707.002

Abstract

Abstract:

OBJECTIVE: To investigate the most effective weight ratio of Qiancao (Radix Rubiae Cordifoliae) and Haipiaoxiao (Endoconcha Sepiellae) used to produce decoction for the treatment of abnormal uterine bleeding (AUB) in rats with incomplete abortion, and to study the possible mechanism.

METHODS: The models of AUB were established by incomplete drug abortion with mifepristone and misoprostol in pregnant rats. The therapeutic effects of decoctions made by eight different weight ratios of Qiancao (Radix Rubiae Cordifoliae) and Haipiaoxiao (Endoconcha Sepiellae) were observed.

RESULTS: Statistical analysis revealed that the most effective weight ratio of Qiancao (Radix Rubiae Cordifoliae) and Haipiaoxiao (Endoconcha Sepiellae) to treat AUB in this study was 2∶1.

CONCLUSION: The insights gained from this study would improve understanding of the mechanisms involved in the effect of Qiancao (Radix Rubiae Cordifoliae) and Haipiaoxiao (Endoconcha Sepiellae) on AUB.

Key words: uterine hemorrhage, Qiancao (Radix Rubiae Cordifoliae), Haipiaoxiao (Endoconcha Sepiellae), herb pair, weight ratio

ZHANG Tiantian, LI Guowen, CHU Zhifeng, LI Jie, YU Lijun, GAO Yuan, WANG Huifeng. Efficacy of Qiancao (Radix Rubiae Cordifoliae) and Haipiaoxiao (Endoconcha Sepiellae), a traditional Chinese herb pair, on abnormal uterine bleeding in rats with incomplete abortion[J]. Journal of Traditional Chinese Medicine, 2022, 42(5): 758-763.

Figures/Tables 4

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Group	n	Bleeding volume (mL)	PT (s)	APTT (s)
GN	10	1.121±0.080^a	18.608±0.731^a	57.715±0.605^a
GP	10	0.590±0.028^b	16.441±0.129^b	53.012±1.030^b
G1	10	0.747±0.036^a,b	15.798±0.120^b,c	49.312±1.689^a,b
G2	10	0.602±0.064^b	14.501±0.097^a,b	43.822±0.768^a,b
G3	10	0.642±0.049^b	14.910±0.242^a,b	48.703±1.002^a,b
G4	10	0.805±0.053^a,b	17.030±0.376^b,c	49.831±0.927^a,b
G5	10	0.802±0.052^a,b	17.024±0.497^b,c	49.203±1.031^a,b
G6	10	0.772±0.050^a,b	16.043±0.236^b	48.768±0.725^a,b
G7	10	0.789±0.029^a,b	16.550±0.576^b	48.130±0.852^a,b
G8	10	0.651±0.053^b	14.904±0.106^a,b	44.528±0.912^a,b

Group	n	Bleeding volume (mL)	PT (s)	APTT (s)
GN	10	1.121±0.080^a	18.608±0.731^a	57.715±0.605^a
GP	10	0.590±0.028^b	16.441±0.129^b	53.012±1.030^b
G1	10	0.747±0.036^a,b	15.798±0.120^b,c	49.312±1.689^a,b
G2	10	0.602±0.064^b	14.501±0.097^a,b	43.822±0.768^a,b
G3	10	0.642±0.049^b	14.910±0.242^a,b	48.703±1.002^a,b
G4	10	0.805±0.053^a,b	17.030±0.376^b,c	49.831±0.927^a,b
G5	10	0.802±0.052^a,b	17.024±0.497^b,c	49.203±1.031^a,b
G6	10	0.772±0.050^a,b	16.043±0.236^b	48.768±0.725^a,b
G7	10	0.789±0.029^a,b	16.550±0.576^b	48.130±0.852^a,b
G8	10	0.651±0.053^b	14.904±0.106^a,b	44.528±0.912^a,b

Group	n	Grade Ⅰ (n)	Grade Ⅱ (n)	Grade Ⅲ (n)	Grade Ⅳ (n)	Grade Ⅴ (n)	Score
GN	10	0	0	0	2	8	4.8±0.4^a
GP	10	2	4	4	0	0	2.2±0.7^b
G1	10	0	0	1	3	6	4.5±0.7^a
G2	10	7	3	0	0	0	1.3±0.4^{b, c}
G3	10	5	3	2	0	0	1.7±0.8^b
G4	10	1	4	3	2	0	2.6±0.9^b
G5	10	0	2	5	3	0	3.1±0.7^{b, c}
G6	10	0	4	4	2	0	2.8±0.7^b
G7	10	1	5	4	0	0	2.3±0.6^b
G8	10	2	6	2	0	0	2.0±0.6^b

Group	n	Grade Ⅰ (n)	Grade Ⅱ (n)	Grade Ⅲ (n)	Grade Ⅳ (n)	Grade Ⅴ (n)	Score
GN	10	0	0	0	2	8	4.8±0.4^a
GP	10	2	4	4	0	0	2.2±0.7^b
G1	10	0	0	1	3	6	4.5±0.7^a
G2	10	7	3	0	0	0	1.3±0.4^{b, c}
G3	10	5	3	2	0	0	1.7±0.8^b
G4	10	1	4	3	2	0	2.6±0.9^b
G5	10	0	2	5	3	0	3.1±0.7^{b, c}
G6	10	0	4	4	2	0	2.8±0.7^b
G7	10	1	5	4	0	0	2.3±0.6^b
G8	10	2	6	2	0	0	2.0±0.6^b

Group	n	MMP1	TXA₂	PGI₂
GN	10	3.850±0.144^a	36.923±1.192	99.636±3.750
GP	10	2.875±0.173^b	40.034±0.562^e	101.511±1.124
G1	10	2.713±0.021^b	37.356±0.785	102.462±2.290
G2	10	2.404±0.120^b,c	40.677±0.923^b	101.055±1.253
G3	10	2.602±0.032^b	38.353±1.082	101.423±2.136
G4	10	2.928±0.172^b	36.832±0.401	101.575±1.326
G5	10	3.004±0.183^b	36.962±0.500	103.040±1.338
G6	10	2.909±0.043^b	36.876±0.701	101.802±1.580
G7	10	3.228±0.151^b,d	36.957±0.722	100.950±1.531
G8	10	3.660±0.265^c	37.321±0.640	101.389±1.378

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