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Journal of Traditional Chinese Medicine ›› 2020, Vol. 40 ›› Issue (6): 917-921.DOI: 10.19852/j.cnki.jtcm.2020.06.003

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Establishment of an in vitro model using the rat alveolar macrophage cell line NR8383

Wang Jiawei, Wang Xuekai, Sui Yu, Zhang Xieyu, Hou Wei   

  1. Department of Oncology, Guanganmen Hospital of Chinese Academy of Chinese Medicine Science;Experiment Research Center, R & D Service Base, Chinese Academy of Chinese Medicine Science;
  • Online:2020-12-25 Published:2020-12-25
  • Supported by:
    Supported by the National Natural Science Foundation of China the Mechanism Underlying Nourishing Yin,Clearing Lung and Activating Blood Circulation to Regulate AMPK Mediated Autophagy in Radiation-induced Lung Injury (No.81673754)

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Abstract: OBJECTIVE: To establish an in vitro model of radiation-induced lung injury using rat lung alveolar macrophages(NR8383).METHODS: Using a medical electronic linear accelerator, cells were irradiated with either 0 Gy or 6 Gy X-rays. At 6, 12, 24, 30 and 48 h, the DNA damage index(8-OHd G) and lipid damage index(MDA)were measured in the two groups. We also determined the levels of tumor necrosis factor-alpha(TNF-α), interleukin-6(IL-6) and transforming growth factor-β(TGF-β).RESULTS: The levels of 8-OHd G and MDA in the 6 Gy irradiation group were higher than those in the0 Gy group at 6, 12, 24, 30 and 48 h after irradiation. The levels reached the highest value-6 h after irradiation, and then gradually decreased. The levels of the inflammatory factors TNF-α, TGF-β and IL-6 were higher in the 6 Gy irradiation group than those in the 0 Gy group at 6, 12, 24, 30 and 48 h after irradiation.CONCLUSION: Six Gy X-ray irradiated NR8383 cells can be used to establish an in-vitro model of radiation-induced lung injury. The levels of 8-OHd G,MDA, TNF-α, TGF-β and IL-6 can be used as effective evaluation indicators.

Key words: Lung injury, Radiation, Macrophage,alveolar, In vitro techniques

Cite this article

Wang Jiawei, Wang Xuekai, Sui Yu, Zhang Xieyu, Hou Wei. Establishment of an in vitro model using the rat alveolar macrophage cell line NR8383[J]. Journal of Traditional Chinese Medicine, 2020, 40(6): 917-921.