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Journal of Traditional Chinese Medicine ›› 2020, Vol. 40 ›› Issue (4): 584-592.DOI: 10.19852/j.cnki.jtcm.2020.04.007

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Efficacy of Lidan Tang on high-fat-diet induced gallstone in mice and possible mechanism

Gao Yan, Liu Caipin, Li Juntong, Zhai Yingying, Lin Meiyu, Wu Qinglin, Chu Shifeng, Zhang Zhao, Li Jianping, Zhou Xin, Li Yueting, Chen Naihong   

  1. State Key Lab of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Neuroscience Center, Chinese Academy of Medical Sciences and Peking Union Medical College;Department of Pharmacy, Xuanwu Hospital of Capital Medical University;Department of Surgery, Beijing Hospital of Integrated Traditional and Western Medicine;Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine;College of Pharmacy, Hunan University of Chinese Medicine;State Key Lab of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica,Neuroscience Center, Chinese Academy of Medical Sciences and Peking Union Medical College;
  • Online:2020-08-15 Published:2020-08-15
  • Supported by:
    the National Natural Science Foundation of China (the Mechanism of Treating Cholelithiasis Based on the Theory of Biliary Diseases Treated by Differentiating Syndromes of the Liver,No. 81473570), the Drug Innovation Major Project (No. 2018ZX09711001-002-007,2018-ZX09711001-003-005,2018ZX09711001-009-013), Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study (BZ0150)

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Abstract: OBJECTIVE: To investigate efficacy of Lidan Tang(LDT) on gallstone induced by high fat diet in mice,and to study its underlying mechanism.METHODS: Mice were fed with high fat diet every day and treated with LDT(9.01 times of human clinic dosage). Mice were randomly divided into 6 groups as control group, gallstone model group(high-fat diet), positive control ursodeoxycholic acid(UDCA) group(80 mg·kg-1·d-1, i.g.), LDT low dose group(6 kg/d, i.g.), LDT middle dose group(12 kg/d, i.g.), and LDT high dose group(24 kg/d, i.g.). The whole experiment was lasted for 4 weeks.The levels of ALT, AST, LDH, CHO, HDL-C and LDL-C in serum were measured, the pathological sections were observed by hematoxylin-eosin staining, the activities of antioxidant enzymes were measured by kits, and the proteins related to oxidative stress and lipid transport were detected by Western blot analysis.RESULTS: LDT could significantly reduce the contents of ALT and AST in serum and improve the pathological tissue of liver. LDT could significantly reduce the content of MDA and LPO, and increase the level of GSH and GSH-PX in liver tissue. The data of Western blot showed that LDT had antioxidant effect promoting Keap1/Nrf2 pathway and regulated the process of lipid transport, which was statistically significant. In addition, LDT treatment inhibited the expression of ATP-binding cassette transports ABCG5/8 in liver, and reduced cholesterol transport from the hepatocytes to the gallbladder.CONCLUSION: LDT has protective effect on gallstones induced by high fat diet in mice, which might be based on the protective effect on liver, including enhancing the antioxidant capacity of liver and reducing the production of lipid peroxides.

Key words: Cholelithiasis, ATP-binding cassette transporters, Oxidative stress, Lipids, Lidan Tang

Cite this article

Gao Yan, Liu Caipin, Li Juntong, Zhai Yingying, Lin Meiyu, Wu Qinglin, Chu Shifeng, Zhang Zhao, Li Jianping, Zhou Xin, Li Yueting, Chen Naihong. Efficacy of Lidan Tang on high-fat-diet induced gallstone in mice and possible mechanism[J]. Journal of Traditional Chinese Medicine, 2020, 40(4): 584-592.