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Journal of Traditional Chinese Medicine ›› 2020, Vol. 40 ›› Issue (2): 197-203.DOI: 10.19852/j.cnki.jtcm.2020.02.003

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Effects of a fermented buckwheat flower and leaf extract on the blood glucose and lipid profile of type 2 diabetic db/db mice

Wang Jianxing, Yu Xiaohan, Jiang Yan, Wang Yan, Li Ying, Han Shuying   

  1. North China University of Science and Technology, Jitang College;North China University of Science and Technology, Clinical Medical College;North China University of Science and Technology,Basic Medical College;North China University of Science and Technology, Basic Medical College;Hebei Key Laboratory on Chronic Diseases;Tangshan Basic Clinical Research Key Laboratory on Chronic Diseases;
  • Online:2020-04-15 Published:2020-04-15
  • Supported by:
    Supported by a Grant from the Scientific Research Project of Hebei Provincial Administration for Traditional Chinese Medicine (Protective Effects and Mechanism of Extract from Fermented Buckwheat Flower and Leaf on Kidney in TypeⅡDiabetic db/db Mice,No. 2017083), the Key Project Plan of Medical Science Research in Hebei Province in 2018 (Protective Effects and Mechanism of Extract From Fermented Buckwheat Flower and Leaf on IsletβCells,No. 201810736)

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Abstract: OBJECTIVE: To determine the efficacy of a fermented buckwheat flower and leaf extract(EFBFL) for the reduction of blood glucose and lipid dysregulation in spontaneously obese type 2 diabetic db/db mice, and to explore the possible mechanisms involved.METHODS: Forty 9-week-old male db/db mice were randomly allocated to a high-dose EFBFL group(EFBFL-H), a low-dose EFBFL group(EFBFL-L),a metformin hydrochloride positive control group(MEG), and a db/db control group(MG), and there was also a db/m negative control group(NCG)(n =10). Oral glucose tolerance tests(OGTT) were performed after 7 weeks of treatment. At the end of 8 weeks of treatment, random blood glucose(RBG),glycosylated hemoglobin(Hb Alc), fasting plasma glucose(FPG), fasting serum insulin(FINS), triglyceride(TG), serum total cholesterol(TC), free fatty acids(FFA), high-density lipoprotein-cholesterol, and low-density lipoprotein-cholesterol(LDL-c) were measured, the homeostasis model assessment-insulin resistance(HOMA-IR) was calculated. Immunohistochemistry and western blotting measured the expression of glucose transporter4(GLUT4) and peroxisome proliferator-activated receptor γ(PPAR-γ) by in skeletal muscle.RESULTS: The MG mice had a significantly increased in RBG, Hb Alc, the HOMA-IR level, the serum of TG, TC, LDL-c, but a decreased in glucose tolerance and the protein expression of GLUT4 and PPAR-γ compared with the NCG. Compared with the MG, EFBFL groups significantly decreased RBG, Hb Alc, and the HOMA-IR level, increased glucose tolerance. Meanwhile EFBFL groups reduced the serum TG, TC, and LDL-c in a dose-dependent manner. In addition, EFBFL increased the protein expression of GLUT4 and PPAR-γ in the skeletal muscle of db/db mice.There was significant difference between the MG group and EFBFL groups.CONCLUSION: These findings suggest that EFBFL has anti-diabetic effects in db/db mice, ameliorating glucose intolerance, lipid dysregulation, and insulin resistance.

Key words: Eriogonum, Diabetes mellitus,type 2, Muscle,skeletal, Glucose transporter type 4, PPAR gamma

Cite this article

Wang Jianxing, Yu Xiaohan, Jiang Yan, Wang Yan, Li Ying, Han Shuying. Effects of a fermented buckwheat flower and leaf extract on the blood glucose and lipid profile of type 2 diabetic db/db mice[J]. Journal of Traditional Chinese Medicine, 2020, 40(2): 197-203.