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Journal of Traditional Chinese Medicine ›› 2017, Vol. 37 ›› Issue (06): 794-803.

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Effect of electroacpuncture of Neiguan(PC 6) and Tianquan(PC 2)on skin temperature, blood perfusion, and adrenoceptor response in rats with acute myocardial ischemia

Wang Dan, Ji Bo, Zhao Guozhen, Yan Mingna, Bai Hongxin, Sun Xiaomin, Dai Jian, Lu Yawen, Liu Yitian, You Min, Wu Jiaojuan, Ge Yunpeng, Su Hang, Cheng Kai   

  1. College of Acupuncture and Massage, Beijing University of Traditional Chinese Medicine;Clinical medical college, Beijing Universityof Traditional Chinese Medicine;
  • Accepted:2017-12-15 Online:2017-12-15 Published:2022-04-04
  • Supported by:
    Supported by the Law of Sympathetic Nerve Reaction and Receptor Molecular Response in Relevant Acupoints in Different Myocardial Conditions(No.81373558)

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Abstract: OBJECTIVE: To investigate the effect of electroacupuncture(EA) of acupoints Neiguan(PC 6) and Tianquan(PC 2) on the skin temperature, blood perfusion, and alpha1-and beta2-adrenoreceptor(α1-AR and β2-AR) protein and m RNA level in rats with acute myocardial ischemia.METHODS: Thirty male adult Wistar rats [(230 ±20) g] were randomly assigned into five groups(n = 6 each): a control group, sham operation group, model group, model group treated with EA at low frequency(L-EA, 2 Hz, 1 m A) and model group treated with EA at high frequency(H-EA, 100 Hz, 1 m A). The rat model was prepared by ligating the left anterior descending coronary artery. Electroacupuncture was performed at the left Neiguan(PC 6) for 20 min daily for 3 d. After the 3 rd time of the treatment, measurements of skin blood perfusion and temperature in Neiguan(PC 6) and Tianquan(PC 2) in all groups were made by laser speckle contrast imaging and infrared thermal image instrument. Real-time PCR and ELISA were used to measure m RNA level and protein level of α1-AR andβ2-AR in the skin tissues of Neiguan(PC 6) and Tianquan(PC 2), respectively. Serum levels of c Tn T and electrocardiogram were used to identify the state of myocardium.RESULTS: In the group receiving electroacupunture at Neiguan(PC 6), compared with control group,the skin temperature, blood perfusion, and β2-AR m RNA and protein level of model group significantly decreased(P < 0.05), α1-AR m RNA and protein level of model group significantly increased(P < 0.05);compared with model group, the skin temperature,blood perfusion, and β2-AR m RNA and protein level of L-EA significantly increased(P < 0.05), α1-AR m RNA and protein level of L-EA and H-EA significantly decreased(P < 0.05). The same trend has taken place in the former four groups of the Tianquan(PC 2).CONCLUSION: Low-or high-frequency electroacupuncture can improve the skin temperature and blood perfusion which may be induced by decreasingthelevelofα1-ARandincreasingthelevelofβ2-AR of the Neiguan(PC 6) and Tianquan(PC 2) in the rat with acute myocaidial ischemia.

Key words: Electroacupuncture, Myocardial ischemia, Point PC 6(Neiguan), Point PC 2(Tianquan), Skin temperature, Skin blood perfusion, Adrenoceptor

Cite this article

Wang Dan, Ji Bo, Zhao Guozhen, Yan Mingna, Bai Hongxin, Sun Xiaomin, Dai Jian, Lu Yawen, Liu Yitian, You Min, Wu Jiaojuan, Ge Yunpeng, Su Hang, Cheng Kai. Effect of electroacpuncture of Neiguan(PC 6) and Tianquan(PC 2)on skin temperature, blood perfusion, and adrenoceptor response in rats with acute myocardial ischemia[J]. Journal of Traditional Chinese Medicine, 2017, 37(06): 794-803.