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Journal of Traditional Chinese Medicine ›› 2017, Vol. 37 ›› Issue (03): 412-420.

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Baicalin and rutin are major constituents in Shuanghuanglian injection involving anaphylactoid reaction

Zhang Qi, Cai Chengke, Li Jian, Wang Penglong, Si Yinchu, Ding Nan, Gao Jing, Zhang Yanqiu, Ma Zhiqiang, Lei Haimin, Li Qiang   

  1. Department of Chemistry of Meteria Medica, School of Chinese Materia Medica, Beijing University of Chinese Medicine;Department of Anatomy, School of Preclinical Medicine, Beijing University of Chinese Medicine;
  • Accepted:2017-06-15 Online:2017-06-15 Published:2022-04-04
  • Supported by:
    Supported by National Science Foundation of China(the Research of Repair Mechanisms Based on the Neural Stem Cells Niche Regulation of Chinese Medicine After Brain Damage,No.81373830), National Science;Technology Major Projects for "Major New Drugs Innovation and Development"(Technology Reform of Shuanghuanglian Powder Injection,No.2011ZX09201-201-15)

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Abstract: OBJECTIVE: To identify the constituents in Shuanghuanglian injection(SHLI) that correlate with anaphylactoid reaction.METHODS: Chemical fingerprints of 10 batches SHLI samples were determined by High Performance Liquid Chromatography(HPLC), and further investigated by similarity analysis. Combined with optical microscopy, both anaphylactoid experiments and confirmatory assay were displayed in Rat basophil leukemia cells(RBL-2H3) to obtain the histamine release inducing by SHLI. The content of histamine was tested by Enzyme-Linked Immuno Sorbent As-say method. Partial least squares regression(PLSR)method and HPLC-DAD-ESI-MSntechnology were conducted to analyze constituents in SHLI involving anaphylactoid reaction.RESULTS: The results of spectrum and effect relationships showed that the eight constituents were positively correlated with anaphylactoid reaction.Among which, nearly 90% of them were identified as baicalin and rutin with PLSR and HPLC-DAD-ESI-MSn. This result was in accordance with confirmatory assay on RBL-2H3 cells.CONCLUSION: Baicalin and rutin from SHLI were the main constituents involving anaphylactoid reaction.

Key words: Anaphylaxis, Purpura, schoenlein-henoch, Mast cells, Histamine release, Chromatography, high pressure liquid, Spectrometry, mass, electrospray Ionization, Shuanghuanglian injection

Cite this article

Zhang Qi, Cai Chengke, Li Jian, Wang Penglong, Si Yinchu, Ding Nan, Gao Jing, Zhang Yanqiu, Ma Zhiqiang, Lei Haimin, Li Qiang. Baicalin and rutin are major constituents in Shuanghuanglian injection involving anaphylactoid reaction[J]. Journal of Traditional Chinese Medicine, 2017, 37(03): 412-420.