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Journal of Traditional Chinese Medicine ›› 2017, Vol. 37 ›› Issue (03): 308-313.

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Velvet antler polypeptide is able to induce differentiation of neural stem cells towards neurons in vitro

Zhang Lihong, Zhuang Zhihong, Sun Yanan, Ma Shuhua, Yang Weifeng, Lei Hongtao, Zuo Junling, Ouyang Jingfeng, Wang Yi   

  1. Graduate School, Guangzhou University of Chinese Medicine;Morphological Laboratory, Expeimental Research Center, Research and Development Service Base, China Academy of Chinese Medical Sciences;First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine;
  • Accepted:2017-06-15 Online:2017-06-15 Published:2022-04-04
  • Supported by:
    Supported by the National Natural Sciences Foundation of China(No.81470171), Basic Science Research Fund from Ministry of Finance of China(No.ZZ2012008 and ZZ2015014)

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Abstract: OBJECTIVE: To investigate the neural differentiation capacity of water extraction of velvet antler.METHODS: Velvet antler(Cervus Nippon Temminck) polypeptide(VAP) was used to differentiate neural stem cells(NSCs) towards neurons in the study. Firstly, we obtain the polypeptides of VAP by water extraction. Secondly, we observed the morphology, assayed the factors in the media by enzyme-linked immunosorbent assay, and detected the special neural molecules by immuno fl uorescence staining. NSCs were cultured on the cell climbing film. After neuronal differentiation, differentiated NSCs were mounted for immunocytochemistry with immunofluorescence technique.RESULTS: The differentiating cells look like neuron,some special factors, such as Glial cell line-derived neurotrophic factor, nerve growth factor, in the media can be detected while differentiated neuron-like cells can express the special neural molecules.CONCLUSION: Differentiation of NSCs towards neurons can be induced by velvet antler polypeptide.

Key words: Cornu cervi pantotrichum, Glial cell line-derived neurotrophic factors, Nerve growth factor, Neural stem cells

Cite this article

Zhang Lihong, Zhuang Zhihong, Sun Yanan, Ma Shuhua, Yang Weifeng, Lei Hongtao, Zuo Junling, Ouyang Jingfeng, Wang Yi. Velvet antler polypeptide is able to induce differentiation of neural stem cells towards neurons in vitro[J]. Journal of Traditional Chinese Medicine, 2017, 37(03): 308-313.