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Journal of Traditional Chinese Medicine ›› 2017, Vol. 37 ›› Issue (02): 236-243.

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External use of Ruyanneixiao cream efficiently blocks precancerous mammary lesions by interfering with glycolysis induced by inhibition of hypoxia inducible factor-1α,hexokinase 2,phosphofructokinase,and pyruvate kinase M2 expression

Li Xiaobo, Ma Min, Zhang Guijuan, Ma Yi, Liao Rui, Chen Ruixue, Yan Xianxin, Bie Fengjie, Huang Maojie, Liang Shijie   

  1. College of Traditional Chinese Medicine,Jinan University;the School Outpatient Department,The First Affiliated Hospital of Jinan University;Bio-Engineering Institute of Jinan University;
  • Accepted:2017-04-15 Online:2017-04-15 Published:2022-04-04
  • Supported by:
    Supported by National Natural Science Foundation Project of China(No.81673979,81473688,81173265), Traditional Chinese Medicine Administration Project of Guangdong Province,China(No.20141070), the Science and Technology Program of Guangdong,China(No.2014A020212672,2014A020210015), the Natural Science Foundation of Guangdong,China(No.2016A030313114,2015A030313333), Scientific Research Cultivation and Innovation of Jinan University Special Fund for Basic Scientific Research of Central University(No.21615464,21615412), the New Century Talent Support Program by the Ministry of Education(No.NCET-13-0827)

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Abstract: OBJECTIVE:To investigate the effect of Ruyanneixiao cream(RYNX) on the expression of hypoxia inducible factor-1α(HIF-1α), hexokinase 2(HK2),phosphofructokinase(PFK), and pyruvate kinase M2(PKM2) mRNA and protein in MCF-10 AT cells and in an animal model of precancerous mammary lesions.METHODS:Following treatment of MCF-10 AT cells with RYNX, tamoxifen(TAM) and YC-1 for 48 h,HIF-1α, HK2, PFK, PKM2 mRNA and protein expression was analyzed.Fifty female SD rats were randomly divided into control, model, TAM, and highand low-dose RYNX groups, with 10 rats in each group.A precancerous mammary lesion model was established for all groups except the control group.High-and low-dose RYNX cream containing TAM was coated on the breasts of animals in the corresponding groups.The rat mammary tissue was removed in the 10 th week and HIF-1α, HK2, PFK,PKM2 mRNA and protein was analyzed.RESULTS:In vitro analyses demonstrated that, compared with the matrix group, HIF-1α, HK2, PFK,PKM2 mRNA and protein expression was significantly decreased in the RYNX group(P < 0.05).Compared with the YC-1 + RYNX group, HK2, PFK,and PKM2 protein expression was significantly reduced in the RYNX group.HIF-1α, HK2, PFK, and PKM2 protein expression was increased significantly in the model group(P < 0.05) compared with the control group, while HIF-1α, HK2, PFK, and PKM2 mRNA and protein expression was significantly decreased in both the high-and low-dose RYNX groups(P < 0.05), with the effect being greater in the high-dose group.CONCLUSION:RYNX can block precancerous breast lesions by decreasing the expression of HK2,PFK, and PKM2 mRNA and protein via inhibition of HIF-1α mRNA and protein overexpression in a dose-dependent manner.

Key words: Ruyanneixiao cream, Precancerous mammary lesions, Glycolysis, Hypoxia-inducible factor 1, alpha subunit, Hexokinase, Phosphofructokinases, Pyruvate kinase

Cite this article

Li Xiaobo, Ma Min, Zhang Guijuan, Ma Yi, Liao Rui, Chen Ruixue, Yan Xianxin, Bie Fengjie, Huang Maojie, Liang Shijie. External use of Ruyanneixiao cream efficiently blocks precancerous mammary lesions by interfering with glycolysis induced by inhibition of hypoxia inducible factor-1α,hexokinase 2,phosphofructokinase,and pyruvate kinase M2 expression[J]. Journal of Traditional Chinese Medicine, 2017, 37(02): 236-243.