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Journal of Traditional Chinese Medicine ›› 2017, Vol. 37 ›› Issue (02): 229-235.

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Baicalin extracted from Huangqin(Radix Scutellariae Baicalensis) induces apoptosis in gastric cancer cells by regulating B cell lymphoma(Bcl-2)/Bcl-2-associated X protein and activating caspase-3 and caspase-9

Wang Hongwei, Li Hailong, Chen Fengqin, Luo Jun, Gu Jing, Wang Huping, Wu Hongyan, Xu Yan   

  1. Clinical Laboratory,The Hospital Affiliated to Gansu University of Chinese Medicine;Key Laboratory for Transfer of Dunhuang Medicine at the Provincial and Ministerial Level;Department of Teaching and Research of Clinical Laboratory,School of Clinical Medicine,Gansu University of Chinese Medicine;Gansu Provincial Key Laboratory of Chinese Medicinal Herbs for Prescription,Innovation and Transformation,Gansu University of Chinese Medicine;
  • Accepted:2017-04-15 Online:2017-04-15 Published:2022-04-04
  • Supported by:
    Supported by the Natural Science Foundation of Gansu Province(No.148RJZA073), the Health Industry Scientific Research Project of Gansu Province(No.GWGL 2013-40), the Openning Plan Project of Key Laboratory for Transfer of Dunhuang Medicine at the Provincial and Ministerial Level(No.DHYX1213-008)

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Abstract: OBJECTIVE:To evaluate the effects of baicalin in human gastric cancer cells, including apoptosis-inducing effects, and to investigate its underlying mechanisms of action.METHODS:Cell proliferation and apoptosis assays were performed to investigate the anti-proliferation effects of baicalin in human gastric cancer BGC-823 and MGC-803 cells.Real time-quantitative polymerase chain reaction and Western blotting analysis were performed to elucidate the molecular mechanisms underlying the anti-tumor properties of baicalin.RESULTS:In BGC-823 and MGC-803 gastric cancer cells treated with 80, 120, and 160 μmol/L baicalin for 48 h, a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay showed that baicalin significantly inhibited cell proliferation in a dose-dependent manner, while flow cytometric analysis demonstrated that baicalin could induce apoptosis, also in a dose-dependent manner.Moreover, baicalin up-regulated the expression of caspase-3, caspase-9, and B cell lymphoma(Bcl-2)-associated X protein and down-regulated the expression of Bcl-2 at both the m RNA and protein level.CONCLUSION:Baicalin has potential as a therapeutic agent for gastric cancer by inducing apoptosis in cancer cells.

Key words: Baicalin, Stomach neoplasms, Apoptosis Lymphoma, B-Cell, Bcl-2-associated X protein, Caspases, effector

Cite this article

Wang Hongwei, Li Hailong, Chen Fengqin, Luo Jun, Gu Jing, Wang Huping, Wu Hongyan, Xu Yan. Baicalin extracted from Huangqin(Radix Scutellariae Baicalensis) induces apoptosis in gastric cancer cells by regulating B cell lymphoma(Bcl-2)/Bcl-2-associated X protein and activating caspase-3 and caspase-9[J]. Journal of Traditional Chinese Medicine, 2017, 37(02): 229-235.