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Journal of Traditional Chinese Medicine >

2025 , Vol. 45 >Issue 4: 730 - 738

DOI: https://doi.org/10.19852/j.cnki.jtcm.2025.04.003

Original Articles

Qufeng Jiejing formula (祛风解痉方) ameliorated the injury of airway smooth muscle cells induced by platelet-derived growth factor-BB through the transforming growth factor-β1/Smads signaling pathway

  • Changzheng FAN ,
  • Qiong ZHANG ,
  • Maorong FAN ,
  • Hongxu MENG ,
  • Xiaodong CONG ,
  • Yiling FAN ,
  • Shasha YUAN ,
  • Qing MIAO
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  • 1 Respiratory Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
    2 Institute of Basic Medical Sciences, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
    3 Respiratory Department, China Academy of Chinese Medical Sciences, Beijing 100091, China
MIAO Qing, Respiratory Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. faanchzh@163.com,Telephone: +86-13552145086; +86-1062835377

Received date: 2024-07-23

  Accepted date: 2024-12-31

  Online published: 2025-07-25

Supported by

Science and Technology Innovation Project of China Academy of Chinese Medical Sciences of Study on the Mechanism of Qufeng Jiejing Formula in Regulating Mitogen-activated Protein Kinase Signaling Pathway to Inhibit Phenotypic Transformation of Airway Smooth Muscle Cells in Asthma(CI2021A01108);Cultivation Project of The National Natural Science Foundation of China of Xiyuan Hospital, China Academy of Chinese Medical Sciences of Research on the Role of Traditional Chinese Medicines-Qufeng Jiejing in the Proliferation, Migration and Phenotypic Transformation of Airway Smooth Muscle Cells in Asthma(XY20-17)

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Abstract

OBJECTIVE: To explore the role and mechanism of Qufeng Jiejing (祛风解痉，QFJJ) formula in the asthma progression.

METHODS: The Bagg Albino/c mice treated with Ovalbumin and AL(OH)3, and airway smooth muscle cells (ASMCs) treated with platelet-derived growth factor (PDGF)-BB to establish a asthma model in vivo and in vitro. The cell morphology was observed with microscope and immunofluorescence staining. The cell viability was assessed with methyl thiazolyl tetrazolium assay. The tumor necrosis factor-αlpha (TNF-α), interleukin-1beta (IL-1β), laminin, fibronectin and collagen IV levels in the ASMCs were detected with corresponding enzyme linked immunosorbent assay kits. Transwell and wound healing assays were conducted to test the cell migration. The TGF-β1, Smad2 and Smad3 levels were measured with Western blot.

RESULTS: We found that QFJJ formula treatment dramatically decreased the cell viability, TNF-α, IL-1β, laminin, fibronectin and collagen IV levels in the PDGF-BB stimulated ASMCs. Additionally, the protein levels of TGF-β1, Smad2 and Smad3 in the PDGF-BB stimulated ASMCs were prominently depleted after QFJJ formula treatment. Besides, SRI treatment neutralized the role of QFJJ formula in the PDGF-BB stimulated ASMCs.

CONCLUSION: QFJJ formula effectively relieved the asthma progression through ameliorate the ASMCs function, which was achieved through suppressing the TGF-β1/Smads signaling pathway.

Key words： asthma; myocytes, smooth muscle; transforming growth factor beta1; Smad proteins; signal transduction; Qufeng Jiejing formula

Cite this article

Changzheng FAN , Qiong ZHANG , Maorong FAN , Hongxu MENG , Xiaodong CONG , Yiling FAN , Shasha YUAN , Qing MIAO . Qufeng Jiejing formula (祛风解痉方) ameliorated the injury of airway smooth muscle cells induced by platelet-derived growth factor-BB through the transforming growth factor-β1/Smads signaling pathway[J]. Journal of Traditional Chinese Medicine, 2025 , 45(4) : 730 -738 . DOI: 10.19852/j.cnki.jtcm.2025.04.003

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