Advanced Search

Journal of Traditional Chinese Medicine >

2025 , Vol. 45 >Issue 2: 266 - 271

DOI: https://doi.org/10.19852/j.cnki.jtcm.20240927.001

Original articles

Effects of Huluan decotion (护卵汤) on cyclophosphamide-induced autoimmune premature ovarian failure in murine models

  • Guiling FENG ,
  • Xiaolin ZHOU ,
  • Chengwan SHEN ,
  • Panxiao LI ,
  • ·Abudula ABULIZI
Expand
  • 1 College of Medicial science, Ningde Normal university, Ningde 352000, China
    2 Fujian Key Laboratory of Toxicology and Pharmacotoxicology, Ningde 352000, China
    3 Nanyang Medical College, Nanyang 473000, China
    4 Department of Intensive care, Ningde Normal College affiliated Ningde Hospital, Ningde 352000, China

Received date: 2024-01-12

  Accepted date: 2024-04-08

  Online published: 2024-09-27

Supported by

Fujian Natural Science Foundation Project: Study on Potential Protein Targets of Huluan Decotion in the Intervention of Premature Ovarian Failure(2021J011173);Major Project Cultivation Plan Project of Ningde Normal University: the Effect of Huluan Decotion on the Decreased Ovarian Reserve Function Induced by Cyclophosphamide is Studied based on Forkhead box L2(2019ZDK06)

Fold

Abstract

OBJECTIVES: To investigate the therapeutic effect of Huluan decotion (护卵汤, HLD) on cyclophosphamide-induced premature ovarian failure (POF) in mice and its regulatory mechanisms.

METHODS: Female BALB/c mice were administered cyclophosphamide and administered received different doses of HLD for 28 d. Levels of sex hormone, such as estradiol (E2), follicle stimulating hormone (FSH) and luteinizing hormone (LH) in the sera, were assessed using enzyme-linked immunosorbent assay (ELISA). Follicular structure variances were observed through hematoxylin and eosin (HE) staining, while Forkhead box L2 (FOXL2) expression were analyzed via immune-ohistochemical staining. The primary mechanism of POF were investigated through Western blot analysis.

RESULTS: E2 levels decreased, and FSH and LH levels increased in POF model mice, but these trends were reversed with HLD or premarin administration, the expressions of WNT family member 4 (Wnt4), β-Catenin and FOXL2 were downregulated in POF model mice, whereas high expression levels were observed in control mice and other groups.

CONCLUSION: HLD effectively treats POF induced with cyclophosphamide in mice by enhancing expressions of Wnt4, β-Catenin and FOXL2.

Key words： primary ovarian insufficiency; prescriptions; cyclophosphamide; wnt4 protein; beta catenin; signal transduction; Huluan decotion

Cite this article

Guiling FENG , Xiaolin ZHOU , Chengwan SHEN , Panxiao LI , ·Abudula ABULIZI . Effects of Huluan decotion (护卵汤) on cyclophosphamide-induced autoimmune premature ovarian failure in murine models[J]. Journal of Traditional Chinese Medicine, 2025 , 45(2) : 266 -271 . DOI: 10.19852/j.cnki.jtcm.20240927.001

References

1. Chon Sj, Umair Z, Yoon Ms. Premature ovarian insufficiency: past, present, and puture. Front Cell Dev Biol 2021; 9: 672890.
2. De Vos M, Devroey P, Fauser Bc. Primary ovarian insufficiency. Lancet 2010; 376: 911- 21.
3. Zhang Cr, Zhu Wn, Tao W, et al. Moxibustion against cyclophosphamide-induced premature ovarian failure in rats through inhibiting NLRP3-/Caspase-1-/GSDMD-Dependent pyroptosis. Evid Based Complement Alternat Med 2021; 2021: 8874757.
4. Bellusci G, Mattiello L, Iannizzotto V, et al. Kinase-independent inhibition of cyclophosphamide-induced pathways protects the ovarian reserve and prolongs fertility. Cell Death Dis 2019; 10: 726.
5. Melekoglu R, Ciftci O, Eraslan S, Cetin A, Basak N. Beneficial effects of curcumin and capsaicin on cyclophosphamide-induced premature ovarian failure in a rat model. J Ovarian Res 2018; 11: 33.
6. Maas A. Hormone therapy and cardiovascular disease: Benefits and harms. Best Pract Res Clin Endocrinol Metab 2021; 35: 101576.
7. Farquhar C, Marjoribanks J, Lethaby A, Suckling JA, Lamberts Q. Long term hormone therapy for perimenopausal and postmenopausal women. Cochrane Database Syst Rev 2017; 104: 93-5.
8. Shareghi-Oskoue O, Aghebati-Maleki L, Yousefi M. Transplantation of human umbilical cord mesenchymal stem cells to treat premature ovarian failure. Stem Cell Res Ther 2021; 12: 454.
9. Liu X, Song Y, Zhou F, et al. Network and experimental pharmacology on mechanism of Si-Wu-tang improving ovarian function in a mouse model of premature ovarian failure induced by cyclophosphamide. J Ethnopharmacol 2023; 301: 115842.
10. Zheng S, Ma M, Chen Y, Li M. Effects of quercetin on ovarian function and regulation of the ovarian PI3K/Akt/FoxO3a signalling pathway and oxidative stress in a rat model of cyclophosphamide-induced premature ovarian failure. Basic Clin Pharmacol Toxicol 2022; 130: 240-53.
11. Guo SJ. Yang Jin's experience of treating infertility and crafts with Shuangbu decoction. Global Tradit Chin Med 2016; 9: 831-3.
12. Feng GL, Zhang LH, Zhou XL, et al. Efficacy of Bushen Jianpi prescription on autoimmune premature ovarian failure in mice. J Tradit Chin Med 2017; 37: 667-74.
13. Feng GL, Zhou XL. Effect of Bushen Jianpi recipe on autoimmune premature ovarian failure of mice. J Tradit Chin Med 2016; 57: 71-5.
14. Zhang XL, Li J, Zhou XL, Chen QL. Effect of Er-Xian decoction on autoimmune premature ovarian failure in mice. J Tradit Chin Med 2021; 41: 725-31.
15. Wang ZY, Li MZ, Li WJ, Ouyang JF, Gou XJ, Huang Y. Mechanism of action of Daqinjiao decoction in treating cerebral small vessel disease explored using network pharmacology and molecular docking technology. Phytomedicine 2023; 108: 154538.
16. Myers M, Britt KL, Wreford NG, Ebling FJ, Kerr JB. Methods for quantifying follicular numbers within the mouse ovary. Reproduction 2004; 127: 569-80.
17. Liu W, Cao JL, Ouyang JF, et al. Chaihu and Longgu Muli decoction demonstrate neuroprotective effects in the rat model of Parkinson's disease with depression by regulating the AMPK/mTOR signaling pathway. Phytomedicine Plus 2023; 3: 100428.
18. Yang X, Yang L. Current understanding of the genomic abnormities in premature ovarian failure: chance for early diagnosis and management. Front Med (Lausanne) 2023; 10: 1194865.
19. Han M, Cheng H, Wang J, et al. Abnormal aggregation of myeloid-derived suppressor cells in a mouse model of cyclophosphamide-induced premature ovarian failure. Gynecol Endocrinol 2019; 35: 985-90.
20. Farhat SA, Jabbari F, Jabbari P, Rezaei N. Targeting signaling pathways involved in primordial follicle growth or dormancy: potential application in prevention of follicular loss and infertility. Expert Opin Biol Ther 2022; 22: 871-81.
21. Zhang C, Xu X. Advancement in the treatment of diminished ovarian reserve by traditional Chinese and Western medicine. Exp Ther Med 2016; 11: 1173-76.
22. Han Y, Wang T, Sun S, Zhai Z, Tang S. Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3. Mol Med Rep 2017; 16: 2856-62.
23. Chen H, Chen Q, Zhu Y, et al. MAP3K1 Variant causes hyperactivation of Wnt4/β-Catenin/FOXL2 signaling contributing to 46,XY Disorders/Differences of sex development. Front Genet 2022; 13: 736988.
24. Gustin SE, Hogg K, Stringer JM, et al. WNT/β-catenin and p27/FOXL2 differentially regulate supporting cell proliferation in the developing ovary. Dev Biol 2016; 412: 250-60.
25. Zhang P, Li T, Wu X, et al. Oxidative stress and diabetes: antioxidative strategies. Front Med 2020; 14: 583-600.
Options
Outlines

/

〈 〉