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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 6: 1137 - 1145

DOI: https://doi.org/10.19852/j.cnki.jtcm.2024.06.004

Research Articles

Bushen Huoxue decoction (补肾活血汤) improves the reproduction of endometriosis-associated infertility by regulating Homeobox A10 and αlpha(v)beta(3) integrin expression

  • Weiwei TANG ,
  • Kaili LIU ,
  • Xumei FAN ,
  • Li ZHU ,
  • Zheng ZENG ,
  • Jiali SUN ,
  • Jie SHI ,
  • Zhenzhen ZHANG ,
  • Tao GUI ,
  • Guiping WAN
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  • 1 Department of Obstetrics and Gynecology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China
    2 Department of Pathology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China

Received date: 2023-11-11

  Accepted date: 2024-05-27

  Online published: 2024-11-12

Supported by

National Natural Science Foundation-funded Project: the Mechanism of Effect of Bushen Huoxue Decotion on Endometrial Receptivity in Endometriosis(81603372);National Natural Science Foundation-funded Project: to Investigate the Mechanism of Bushen Huoxue Decoction in Improving Endometrial Receptivity Based on SUMOylation of Homeobox A10(82274190);National Natural Science Foundation-funded Project: to Investigate the Mechanism of Bushen Huoxue Decoction in Improving Endometrial Receptivity Based on SUMOylation of Homeobox A10(M2022079)

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Abstract

OBJECTIVE: To investigate the mechanism of Bushen Huoxue decoction (补肾活血汤, BSHXD) to treat endometriosis-induced infertility.

MEDHODS: The main compounds of BSHXD were determined by high performance liquid chromatography-mass spectrometry (HPLC-MS/MS). The effect of BSHXD on Homeobox A10 (HOXA10) and alpha(v)beta(3) (αvβ3) integrin expression of Ishikawa cells, mouse model, and endometriosis-associated infertility women was evaluated by using Western blot analysis, immunohistochemistry and Real-Time quantitative polymerase chain reaction (RT-qPCR). The efficacy of BSHXD on embryo attachment were examined by using the BeWo spheroid and mouse embryo attachment assay. HOXA10 concentration in uterine flushing fluid of endometriosis-associated infertility women treated with BSHXD was measured by Enzyme-Linked immunosorbent assay (ELISA).

RESULTS: BSHXD improved BeWo spheroid and mice blastocysts attachment to Ishikawa cells and increased embryo implantation rates in mice and pregnancy rates in women with endometriosis-associated infertility. BSHXD enhanced HOXA10 and αvβ3 integrin expression in Ishikawa cell, endometriosis mouse model, and endometriosis-associated infertility women, which potentially improved endometrial receptivity.

CONCLUSIONS: BSHXD could improve endometrial receptivity of endometriosis-associated infertility in a dose-dependent manner by regulating HOXA10 and αvβ3 integrin expression.

Key words： infertility; endometriosis; homeobox A10 proteins; integrin alphaVbeta3; Bushen Huoxue decoction

Cite this article

Weiwei TANG , Kaili LIU , Xumei FAN , Li ZHU , Zheng ZENG , Jiali SUN , Jie SHI , Zhenzhen ZHANG , Tao GUI , Guiping WAN . Bushen Huoxue decoction (补肾活血汤) improves the reproduction of endometriosis-associated infertility by regulating Homeobox A10 and αlpha(v)beta(3) integrin expression[J]. Journal of Traditional Chinese Medicine, 2024 , 44(6) : 1137 -1145 . DOI: 10.19852/j.cnki.jtcm.2024.06.004

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