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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 5: 916 - 925

DOI: https://doi.org/10.19852/j.cnki.jtcm.20240719.001

Huaiyu pill (槐榆片) alleviates inflammatory bowel disease in mice via blocking toll like receptor 4/ myeloid differentiation primary response gene 88/ nuclear factor kappa B subunit 1 pathway

  • Chunyan YANG ,
  • Jia LUO ,
  • Weijie PENG ,
  • Weibo DAI
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  • 1 Department of Pharmacy, Zhongshan Hospital of Traditional Chinese Medicine, Guangzhou University of Traditional Chinese Medicine, Zhongshan 528400, China
    2 Department of Pharmacy, Shenshan Medical Center Memorial Hospital of Sun Yat-Sen University Sun Yat-Sen University, Shanwei 516600, China

Received date: 2024-01-22

  Accepted date: 2024-05-15

  Online published: 2024-07-19

Supported by

Mechanistic Study on the Regulation of Mucin 2/interleukin 6-signal Transducer and Activator of Transcription 3 Signaling Pathway by Active Saponin Ardisiacrispin B in Improving Ulcerative Colitis Intestinal Mucosal Barrier Dysfunction(A2022479);Mechanistic Study on the Regulation of Inflammatory Microenvironment and Improvement of Ulcerative Colitis by Lingnan Traditional Medicine Ficus Quercifolia through Wilms' tumor 1-associating Protein-Mediated RNA Methyltransferase Promoting Toll Like Receptor 4 m6A Modification(2023A1515011699)

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Abstract

OBJECTIVE: To investigate the therapeutic effects of Huaiyu pill (槐榆片, HYP) on inflammatory bowel disease (IBD) and the underlying mechanisms have not been elucidated.

METHODS: To establish the IBD model, mice were administered with dextran sulfate sodium (DSS). Mice were intragastrically pre-treated with sulfasalazine (SASP) and HYP. Disease activity index (DAI) and colon length were monitored, and the colonic tissues were subjected to hematoxylin-eosin staining. Pro-inflammatory factors and vascular inflammation-related proteins were determined using enzyme-linked immunosorbent assay (ELISA). The potential mechanisms of HYP were examined using network pharmacology analysis.The expressions of zona occludens 1 (ZO-1), occludin, toll like receptor 4 (TLR4), myeloid differentiation primary response gene 88 (MYD88), and nuclear factor kappa B p65 subunit (NF-κB p65) in colon tissues were examined using Western blotting or immunohistochemical analyses.

RESULTS: Pre-treatment with HYP enhanced the colon length, decreased DAI scores, and mitigated histopathological alterations in DSS-treated mice. HYP alleviated intestinal inflammation by downregulating the levels of interleukin 1 beta (IL-1β), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α) and interleukin 17 (IL-17). Additionally, HYP suppressed the disruption of the gut barrier by upregulating the ZO-1, occludin, and mucin 2 (MUC2) levels and downregulating the endothelin 1 (ET-1) and erythropoietin (EPO) levels. Network pharmacological analysis and experimental results revealed that HYP downregulated the colonic tissue levels of TLR4, MYD88, and NF-κB p65 in DSS-treated mice.

CONCLUSION: This study investigated the in vivotherapeutic effects of HYP on IBD and the underlying molecular mechanisms. These findings provide an experimental foundation for the clinical application of HYP.

Key words： inflammatory bowel diseases; network pharmacology; intestinal inflammation; intestinal damage; Huaiyu pill

Cite this article

Chunyan YANG , Jia LUO , Weijie PENG , Weibo DAI . Huaiyu pill (槐榆片) alleviates inflammatory bowel disease in mice via blocking toll like receptor 4/ myeloid differentiation primary response gene 88/ nuclear factor kappa B subunit 1 pathway[J]. Journal of Traditional Chinese Medicine, 2024 , 44(5) : 916 -925 . DOI: 10.19852/j.cnki.jtcm.20240719.001

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