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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 4: 745 - 752

DOI: https://doi.org/10.19852/j.cnki.jtcm.2024.04.002

Original articles

Effect of moxibustion on expression profile of miRNAs in Tripterygium glycoside-induced decreased ovarian reserve

  • Jie SHEN ,
  • Yaoli YIN ,
  • Hongxiao LI ,
  • Ge LU ,
  • Yaoyao ZHU ,
  • Yantong QIN ,
  • Xun JIN ,
  • Jie CHENG ,
  • Meihong SHEN
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  • College of Acupuncture and Tuina, Nanjing University of Chinese Medicine, Nanjing 210023, China

Received date: 2023-03-02

  Accepted date: 2023-07-20

  Online published: 2024-07-15

Supported by

National Natural Science Foundation of China: Based on Phosphatidylinositol-3-kinase/Protein kinase B Signaling Pathway Study microRNAs Regulating Mechanism of Moxibustion Improve Ovarian Function(81804179);Explore the miRNA Mechanism of Acupuncture and Moxibustion Against Oxygen Stress in Improving Ovarian Function based on Nuclear Factor Erythropoietin-2-related Factor 2/AU-rich Element Pathway(81774408);Explore the Effect and Molecular Mechanism of Moxibustion on Granulosa Cells in Premature Ovarian Failure based on "Autophagy-apoptosis Cross-talk"(81973957)

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Abstract

OBJECTIVE: To explore the possible regulatory mechanism of microRNA (miRNA) in moxibustion treatment for decreased ovarian reserve (DOR).

METHODS: The DOR model was constructed by intragastrical Tripterygium glycoside suspension administration, and moxibustion therapy was simultaneously given. The morphological ovarian changes were observed by hematoxylin and eosin staining. The miRNA expression profile was detected by RNA sequencing, and bioinformatics analysis was performed. Cytoscape software 3.6.1 was used to establish a regulatory network and differentially expressed miRNAs were verified by reverse transcription quantitative polymerase chain reaction (RT-qPCR).

RESULTS: Decreased number of mature follicles, increased atresia follicles, and abnormal granulosa cell morphology were observed in the model group compared with the control group. The moxibustion group demonstrated increased mature follicles, decreased atretic follicles, and significantly decreased abnormal morphology of granulosa cells compared with the model group. Additionally, RNA sequencing results manifested significantly up-regulated miRNA expressions (miR-92b-3p, miR-26-5p_R + 1_1ss10TC, miR-206-3p, miR-9993b-3p_1ss6GA, miR-7857-3p_R-1, miR-219a-2-3p_1ss10GC, miR-3968-p5_1ss10AT, and PC-5p-6478_1795) and down-regulated miR-664-2-5p_R + 1 in the model group, compared with the control group, and the moxibustion group reversed abnormal disorder levels of these miRNAs. Moreover, these differentially expressed miRNAs were mainly involved in the phosphatidylinositol-3-kinase / protein kinase B signaling pathway and nuclear factor erythropoietin-2-related factor 2 / heme oxygenase 1 signaling pathway. Finally, network and RT-qPCR verification revealed miR-9993b-3p_1ss6GA as the most critical miRNA.

CONCLUSION: This experiment proved the effectiveness of moxibustion in improving the ovarian reserve of rats by regulating miRNA expression, especially miR-9993b-3p_1ss6GA.

Key words： ovarian reserve; moxibustion; microRNAs; mechanics

Cite this article

Jie SHEN , Yaoli YIN , Hongxiao LI , Ge LU , Yaoyao ZHU , Yantong QIN , Xun JIN , Jie CHENG , Meihong SHEN . Effect of moxibustion on expression profile of miRNAs in Tripterygium glycoside-induced decreased ovarian reserve[J]. Journal of Traditional Chinese Medicine, 2024 , 44(4) : 745 -752 . DOI: 10.19852/j.cnki.jtcm.2024.04.002

References

1. Greene AD, Patounakis G, Segars JH. Genetic associations with diminished ovarian reserve: a systematic review of the literature. J Assist Reprod Genet 2014; 31: 935-46.
2. Zhao M, Feng F, Chu C, Yue W, Li L. A novel EIF4ENIF1 mutation associated with a diminished ovarian reserve and premature ovarian insufficiency identified by whole-exome sequencing. J Ovarian Res 2019; 12: 119.
3. Tan Z, Gong X, Wang CC, et al. Diminished Ovarian Reserve in Endometriosis: Insights from In Vitro, In Vivo, and Human Studies-A Systematic Review. Int J Mol Sci 2023; 24(21): 15967.
4. Jin X, Cheng J, Shen J, et al. Moxibustion improves ovarian function based on the regulation of the androgen balance. Erratum in: Exp Ther Med 2022; 23:190.
5. Shen J, Ai B, Shen M. Effectiveness of mild moxibustion for sub-health conditions in pre- and post-menopausal women: a randomized controlled clinical trial. Med Sci Monit 2018; 24: 2907-11.
6. Zhao QY, Sun Y, Zhou J, et al. Effectiveness of herb-partitioned moxibustion combined with electroacupuncture on polycystic ovary syndrome in patients with symptom pattern of kidney deficiency and phlegm-dampne. J Tradit Chin Med 2021; 41: 985-93.
7. Yang X, Wang W, Zhang Y, Wang J, Huang F. Moxibustion improves ovary function by suppressing apoptosis events and upregulating antioxidant defenses in natural aging ovary. Life Sci 2019; 229: 166-72.
8. Thabet E, Yusuf A, Abdelmonsif DA, Nabil I, Mourad G, Mehanna RA. Extracellular vesicles miRNA-21: a potential therapeutic tool in premature ovarian dysfunction. Mol Hum Reprod 2020; 26: 906-19.
9. Zhang J, Xu Y, Liu H, Pan Z. MicroRNAs in ovarian follicular atresia and granulosa cell apoptosis. Reprod Biol Endocrinol 2019; 17: 9.
10. Wei C, Xiang S, Yu Y, Song J, Zheng M, Lian F. miR-221-3p regulates apoptosis of ovarian granulosa cells via targeting FOXO1 in older women with diminished ovarian reserve (DOR). Mol Reprod Dev 2021; 88: 251-60.
11. Wang ZQ, Cui YH, Huang Y, et al. Herb-partitioned moxibustion regulated the miRNA expression profile in the thyroid tissues of rats with experimental autoimmune thyroiditi. J Tradit Chin Med 2021; 41: 789-98.
12. Sun Y, Chen S, Tan Y. Influence of nourishing Yin and tonifying Yang sequential therapy combined with Western Medicine on TGF-β1/Smads signaling pathway in anovulatory infertility rats with diminished ovarian reserve. Zhong Nan Da Xue Xue Bao Yi Xue Ban 2018; 43: 1068-74.
13. Hirshfield AN, Midgley AR Jr. Morphometric analysis of follicular development in the rat. Biol Reprod 1978; 19: 597-605.
14. Tilly JL. Ovarian follicle counts--not as simple as 1, 2, 3. Reprod Biol Endocrinol 2003; 1: 11.
15. Liu W, Chen Q, Liu Z, et al. Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis. J Ethnopharmacol 2021; 277: 113789.
16. Wang Q, Lu G, Xie Z, Xie ZJ, Li HX, Shen MH. Effect of moxibustion on Nrf2/HO-1 signaling pathway in rats with diminished ovarian reserve. Zhong Guo Zhen Jiu 2021; 41: 53-8.
17. Xiao J, Song J, Sa Y, Yuan L, Guo J, Sun Z. The mechanisms of improving IVF outcomes of Liu-Wei-Di-Huang pill acting on DOR patients. Evid Based Complement Alternat Med 2020; 2020: 5183017.
18. Yang LJ, Wu J, Yang L, Zhou T, Li HR, Miao RQ. Effect on follicular development and pregnancy outcome treated with acupuncture and moxibustion therapy of Tiaochongren Gushenyuan in patients with luteal phase defect. Zhong Guo Zhen Jiu 2019; 39: 927-31.
19. Li Y, Xia G, Tan Y, Shuai J. Acupoint stimulation and Chinese herbal medicines for the treatment of premature ovarian insufficiency: a systematic review and Meta-analysis. Complement Ther Clin Pract 2020; 41: 101244.
20. Ren Y, Yang X, Zhang Y, Wang Y, Li X. Effects and mechanisms of acupuncture and moxibustion on reproductive endocrine function in male rats with partial androgen deficiency. Acupunct Med 2016; 34: 136-43.
21. Fan H, Ji F, Lin Y, et al. Electroacupuncture stimulation at CV4 prevents ovariectomy-induced osteoporosis in rats via Wnt-β-catenin signaling. Mol Med Rep 2016; 13: 2485-91.
22. Fu H, Sun J, Tan Y, et al. Effects of acupuncture on the levels of serum estradiol and pituitary estrogen receptor beta in a rat model of induced super ovulation. Life Sci 2018; 197: 109-13.
23. Du Y, Liu R, Sun G, Meng P, Song J. Pre-moxibustion and moxibustion prevent Alzheimer's disease. Neural Regen Res 2013; 8: 2811-9.
24. Li HX, Shi L, Liang SJ, et al. Moxibustion alleviates decreased ovarian reserve in rats by restoring the PI3K/AKT signaling pathway. J Integr Med 2022; 20: 163-72.
25. Yin Y, Li H, Qin Y, et al. Moxibustion mitigates mitochondrial dysfunction and NLRP 3 inflammatory activation in cyclophosphamide-induced premature ovarian insufficiency rats. Life Sci 2023; 314: 121283.
26. Zhang YR, Huang W, Wang LH, Yu BL, Zhang YJ, Zhou ZY. Summary of professor Zhou Zhongyu's academic experience in the treatment of decreased ovarian reserve function with Yin-Yang regulating moxibustion. Zhen Jiu Lin Chuang Za Zhi 2023; 39: 86-90.
27. Poulakos L, Kent TH. Gastric emptying and small intestinal propulsion in fed and fasted rats. Gastroenterology 1973; 64: 962-7.
28. Lu G, Wang Q, Xie ZJ, et al. Moxibustion ameliorates ovarian reserve in rats by mediating Nrf2/HO-1/NLRP 3 anti-inflammatory pathway. Evid Based Complement Alternat Med 2021; 2021: 8817858.
29. Zheng Y, Feng X, Mi H, et al. Effects of transcutaneous electrical acupoint stimulation on ovarian reserve of patients with diminished ovarian reserve in in vitro fertilization and embryo transfer cycles. J Obstet Gynaecol Res 2015; 41: 1905-11.
30. Zhu S, Wang Y, Chang X, Chen H, Jin X. The Protective effect of pre-moxibustion on reproductive hormones profile of rats with tripterygium glycosides-induced ovarian damage. Complement Med Res 2020; 27: 401-9.
31. Hewlett M, Mahalingaiah S. Update on primary ovarian insufficiency. Curr Opin Endocrinol Diabetes Obes 2015; 22: 483-9.
32. Abu-Halima M, Becker LS, Ayesh BM, et al. Characterization of micro-RNA in women with different ovarian reserve. Sci Rep 2021; 11: 13351.
33. Zhao C, Li X, Yang Y, et al. An analysis of Treg/Th 17 cells imbalance associated microRNA networks regulated by moxibustion therapy on Zusanli (ST36) and Shenshu (BL23) in mice with collagen induced arthritis. Am J Transl Res 2019; 11: 4029-45.
34. Bouazzi L, Sproll P, Eid W, Biason-Lauber A. The transcriptional regulator CBX2 and ovarian function: a whole genome and whole transcriptome approach. Sci Rep 2019; 9: 17033.
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