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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 3: 437 - 447

DOI: https://doi.org/10.19852/j.cnki.jtcm.20240423.004

Transient receptor potential vanilloid 1 involved in the analgesic effects of total flavonoids extracted from Longxuejie (Resina Dracaenae Cochinchinensis)

  • Xiaoqiang MO ,
  • Yating CHEN ,
  • Qian YIN ,
  • Haibo CHEN ,
  • Qiang BAN ,
  • Jun LI ,
  • Su CHEN ,
  • Jinguang YAO
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  • 1 Department of Stomatology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, China
    2 College of General Practitioners, Youjiang Medical University for Nationalities, Baise 533000, China
    3 College of Biomedical Engineering, South-Central Minzu University, Key Laboratory of Cognitive Science of State Ethnic Affairs Commission, Hubei Key Laboratory of Medical Information Analysis and Tumor Diagnosis and Treatment, Wuhan 430074, China
    4 College of Pharmacy, South-Central Minzu University, Wuhan 430074, China
Prof. CHEN Su, College of Biomedical Engineering, South-Central Minzu University, Wuhan 430074, China. ecloud.7@163.com Telephone: +86-27-67841502
Prof. YAO Jinguang, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, China. yao7760698@126.com;

Received date: 2023-02-22

  Accepted date: 2023-07-06

  Online published: 2024-04-30

Supported by

High Level Talents Project of Affiliated Hospital of Youjiang Medical University for Nationalities: Study of Soft-Du'an Capsule's Mechanism and Efficacy of Regulating TRPV1 Pashways in Relieving Oral and Maxillofacial Trigeminal Neuralgia(YYFYR20213002);Innovative Group Project of Natural Science Foundation of Hubei Province: Study on the Mechanisms of Pain Signal Transduction and Drug Analgesia(2020CFA025)

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Abstract

OBJECTIVE: To evaluate the analgesic effects of total flavonoids of Longxuejie (Resina Dracaenae Cochinchinensis) (TFDB) and explore the possible analgesic mechanism associated with transient receptor potential vanilloid 1 (TRPV1).

METHODS: Whole-cell patch clamp technique was used to observe the effects of TFDB on capsaicin-induced TRPV1 currents. Rat experiments in vivo were used to observe the analgesic effects of TFDB. Western blot and immunofluorescence experiments were used to test the change of TRPV1 expression in DRG neurons induced by TFDB.

RESULTS: Results showed that TFDB inhibited capsaicin-induced TRPV1 receptor currents in acutely isolated dorsal root ganglion (DRG) neurons of rats and the half inhibitory concentration was (16.7 ± 1.6) mg/L. TFDB (2-20 mg/kg) showed analgesic activity in the phase Ⅱ of formalin test and (0.02-2 mg per paw) reduced capsaicin-induced licking times of rats. TFDB (20 mg/kg) was fully efficacious on complete Freund's adjuvant (CFA)-induced inflammatory thermal hyperalgesia and capsaicin could weaken the analgesic effects. The level of TRPV1 expressions of DRG neurons was also decreased in TFDB-treated CFA-inflammatory pain rats.

CONCLUSION: All these results indicated that the analgesic effect of TFDB may contribute to their modulations on both function and expression of TRPV1 channels in DRG neurons.

Key words： Freund's adjuvant; formaldehyde; capsaicin; flavonoids; Longxuejie (Resina Dracaenae Cochinchinensis); transient receptor potential vanilloid 1; inflammatory pain

Cite this article

Xiaoqiang MO , Yating CHEN , Qian YIN , Haibo CHEN , Qiang BAN , Jun LI , Su CHEN , Jinguang YAO . Transient receptor potential vanilloid 1 involved in the analgesic effects of total flavonoids extracted from Longxuejie (Resina Dracaenae Cochinchinensis)[J]. Journal of Traditional Chinese Medicine, 2024 , 44(3) : 437 -447 . DOI: 10.19852/j.cnki.jtcm.20240423.004

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