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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 1: 145 - 155

DOI: https://doi.org/10.19852/j.cnki.jtcm.20231110.006

Original articles

Efficacy of Tounongsan decoction (透脓散方) on pyogenic liver abscess: network pharmacology and clinical trial validation

  • Qi ZHANG ,
  • Dexuan CHEN ,
  • Guixiang ZHU ,
  • Shihu ZHANG ,
  • Xiao FENG ,
  • Chaoqun MA ,
  • Yi ZHANG
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  • 1 Department of General Surgery, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing 210029, China; Zhuzhou Orthopaedic Hospital of Traditional Chinese Medicine, Zhuzhou 412300, China
    2 Department of General Surgery, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing 210029, China
    3 Department of General Surgery, Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing 210029, China

Received date: 2022-09-11

  Accepted date: 2022-12-17

  Online published: 2023-11-10

Supported by

Jiangsu Provincial Administration of Traditional Chinese Medicine: Clinical Study of TNS Combined with Puncture Catheter Drainage in the Treatment of PLA(YB201814);Natural Fund of Nanjing University of Chinese Medicine: Study on Promoting Glycolysis and Regulating Immune Microenvironment in Gastric Cancer MAIT Cells(ZR2021016);Natural Science Foudation of Jiangsu Province: the DextRan/Sore Spirit Complex Hydrogel was created in a Multicenter Study in the Treatment of Chronic Refractory Sores(BE2022819)

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Abstract

OBJECTIVE: To elucidate the molecular mechanisms governing the effect of Tounongsan decoction (透脓散方, TNS) on the pyogenic liver abscess.

METHODS: Based on oral bioavailability and drug-likeness, the main active components of TNS were screened using the Traditional Chinese Medicine Systems Pharmacology platform. The GeneCard and UniProt databases were used to establish a database of pyogenic liver abscess targets. The interactive network map of drug-ingredients-target-disease was constructed using Cytoscape software (Version 3.7.2). A protein-protein interaction network was constructed using the STRING database, and the related protein interaction relationships were analyzed. biological process of gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed for the core targets. Finally, a clinical trial was performed to verify the reliability of the network pharmacology.

RESULTS: Forty active components of TNS decoction were obtained, and 61 potential targets and 11 proteins were identified. Pathways involved in the treatment of pyogenic liver abscess include the phosphatidylinositide 3-kinases-protein kinase B (PI3K-AKT), advanced glycation end products-receptor for advanced glycation end products (AGE-RAGE), and tumor necrosis factor (TNF) signaling pathways. The results of network pharmacology analysis combined with clinical trials validated that TNS decoction could alleviate the inflammatory response of pyogenic liver abscesses by decreasing interleukin 6 (IL-6) levels.

CONCLUSIONS: TNS decoction has the characteristics of being multi-system, multi-component, and multi-target. Active ingredients in TNS, such as quercetin, kaempferol, fisetin, and β-sitosterol, have strong potential to be candidate drugs for treating pyogenic liver abscesses. The possible mechanism of TSN decoction includes regulating immune and inflammatory responses and reducing IL-6 production to control inflammatory development.

Key words： network pharmacology; clinical trial; liver abscess, pyogenic; Tounongsan decoction

Cite this article

Qi ZHANG , Dexuan CHEN , Guixiang ZHU , Shihu ZHANG , Xiao FENG , Chaoqun MA , Yi ZHANG . Efficacy of Tounongsan decoction (透脓散方) on pyogenic liver abscess: network pharmacology and clinical trial validation[J]. Journal of Traditional Chinese Medicine, 2024 , 44(1) : 145 -155 . DOI: 10.19852/j.cnki.jtcm.20231110.006

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