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Journal of Traditional Chinese Medicine >

2024 , Vol. 44 >Issue 1: 113 - 121

DOI: https://doi.org/10.19852/j.cnki.jtcm.20231110.004

Original articles

Effect of electroacupuncture at Neiguan (PC6) at different time points on myocardial ischemia reperfusion arrhythmia in rats

  • Qianhui SUN ,
  • Kai CHENG ,
  • Xingye DAI ,
  • Zhiwen YANG ,
  • Xiaoling WU ,
  • Chang XU ,
  • Xinghua QIU ,
  • Xiaofeng GAO ,
  • Daonan LIU ,
  • Qirui YANG
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  • 1 School of Acupuncture and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China
    2 School of Acupuncture and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China
    3 Beijing MedEx Technology Co., Ltd., Beijing 100029, China

Received date: 2023-01-22

  Accepted date: 2023-05-17

  Online published: 2023-11-10

Supported by

Transversal Project: Scenario-based Application of Wearable Devices for the Treatment of Cardiac Arrhythmias with Superficial Stimulation at the Neiguan (PC6) Point Research(BUCM-2022-JS-FW-003)

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Abstract

OBJECTIVE: To observe the effects of electroacupuncture at Neiguan (PC6) at different time points on reperfusion arrhythmia (RA) after myocardial ischemia and reperfusion in rats, and to investigate the correlation of this protective effect with nerve growth factor (NGF), tyrosine kinase A (TrkA), tyrosine hydroxylase (TH), and norepinephrine (NE).

METHODS：A total of 72 Sprague-Dawley male rats were randomly divided into six groups (n = 12 rats/group): normal group (Norm), sham operation group (Sham), ischemia reperfusion group (I/R), pre-ischemic electroacupuncture group (EAI), pre-reperfusion electroacupuncture group (EAII), post-reperfusion electroacupuncture group (EAIII). The myocardial ischemia-reperfusion injury (MIRI) model was induced by occlusion of left anterior descending coronary artery for 20 min followed by reperfusion for 40 min in rats. With no intervention in the Norm group and only threading without ligation in the Sham group. Electroacupuncture pre-treatment at 20 min/d for 7 d before ligation in the EAⅠ group, 20 min of electroacupuncture before reperfusion in the EAII group and 20 min of electroacupuncture after reperfusion in the EAIII group. The electrocardiogram (ECG) of each group was recorded throughout the whole process, and the success of the MIRI model was determined based on the changs of J-point and T-wave in the ECG. The arrhythmia score was used to record premature ventricular contractions, ventricular tachycardia and ventricular fibrillation during the reperfusion period to assess the reperfusion induced arrhythmias. The expression levels of NGF, TrkA, TH protein were measured by Western blot. Moreover, the expression levels of plasma and myocardial NE levels were detected by enzyme linked immunosorbent assay.

RESULTS: The differences between Norm group and Sham group were not statistically significant in all indexes. Arrhythmia score, myocardial NGF, TrkA, TH, and NE expression were significantly higher in the I/R group compared with the Sham group. Arrhythmia score, myocardial NGF, TrkA, TH, and NE expression were significantly lower in each EA group compared with the I/R group.

CONCLUSION: Electroacupuncture at Neiguan (PC6) at different time points can reduce the incidence and severity of reperfusion arrhythmias in rats. This protective effect is related to electroacupuncture regulating NGF, TrkA, TH, NE expression and reducing sympathetic hyperactivation.

Key words： myocardial reperfusion injury; reperfusion arrhythmia; electroacupuncture; point PC6 (Neiguan); nerve growth factor; protein-tyrosine kinases; norepinephrine

Cite this article

Qianhui SUN , Kai CHENG , Xingye DAI , Zhiwen YANG , Xiaoling WU , Chang XU , Xinghua QIU , Xiaofeng GAO , Daonan LIU , Qirui YANG . Effect of electroacupuncture at Neiguan (PC6) at different time points on myocardial ischemia reperfusion arrhythmia in rats[J]. Journal of Traditional Chinese Medicine, 2024 , 44(1) : 113 -121 . DOI: 10.19852/j.cnki.jtcm.20231110.004

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