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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 6: 1200 - 1208

DOI: https://doi.org/10.19852/j.cnki.jtcm.20231008.002

Research Articles

Bo′s abdominal acupuncture improves disordered metabolism in obese type 2 diabetic rats through regulating fibroblast growth factor 21 and its related adipokines

  • Xihui QIN ,
  • Jianli PANG ,
  • Guan XIONG ,
  • Jie FENG
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  • 1 School of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, China
    2 Department of Endocrinology, Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine, Nanning 530011, China
    3 Department of Gynecology and obstetrics, the Eighth People’s Hospital of Nanning, Nanning 530007, China
    4 School of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, China

Received date: 2022-08-11

  Accepted date: 2022-11-23

  Online published: 2023-10-08

Supported by

National Natural Science Foundation of China(81560731);to Explore the Mechanism of the Regulation of Bo′s Abdominal Acupuncture on Obesity Type 2 Diabetes based on fibroblast growth factor 21 Signaling Pathway

Fold

Abstract

OBJECTIVE: To investigate the effect of Bo′s abdominal acupuncture (BOAA) on fibroblast growth factor 21 (FGF21) and its related adipokines in type 2 diabetes mellitus (T2DM) rats.
METHODS: This study established obese T2DM rat model by high-fat diet (HFD) with a dose of streptozotocin (STZ, 30 mg/kg). Obese T2DM rats were randomly subdivided into four groups (n = 10): negative, BOAA, conventional acupuncture (COA group) and metformin group (Met group) groups. The biochemical parameters, mRNAs, and proteins were analyzed using enzyme-lined immunoassays kits, quantitative polymerase chain reaction and Western blot.
RESULTS: Treatment with BOAA attenuated the histopathological changes in visceral fat and restored the alterations in the levels of body weight, fasting blood glucose (FBG), homeostasis model assessment for insulin resistance (HOMA-IR). BOAA treatment significantly decreased the levels of triglyceride, total cholesterol, low density lipoprotein cholesterol, leptin, and increased the serum levels of high-density lipoprotein cholesterol, fibroblast growth factor 21 (FGF21), adiponectin (ADP), peroxisome proliferator-activated receptor γ (PPAR-γ), C-peptide (C-P) in obese T2DM rats. Furthermore, BOAA treatment significantly increased the mRNA expressions of FGF21, ADP, leptin, PPAR-γ, PPAR-α and adenosine 5‘-monophosphate (AMP)-activated protein kinase (AMPK). Besides, BOAA treatment upregulated the protein expressions of fibroblast growth factor receptors3 (FGFR3), PPAR-α, extracellular signal-regulated kinase (ERK), phosphorylated ERK (p-ERK), AMPK, p-AMPK, Liver kinase B1 (LKB1), phosphorylated LKB1 (p-LKB1), acetyl-CoA carboxylase (ACC) and phosphorylated ACC (p-ACC), while downregulated the protein expressions of FGF21 and PPAR-γ in visceral fat.
CONCLUSIONS: BOAA treatment reduced FBG and body weight, and improved insulin sensitivity through regulating FGF21 signaling pathway and its related adipokine in obese T2DM rats.

Key words： diabetes mellitus, type 2; obesity; fibroblast growth factors; adipokines; insulin resistance; Bo′s abdominal acupuncture

Cite this article

Xihui QIN , Jianli PANG , Guan XIONG , Jie FENG . Bo′s abdominal acupuncture improves disordered metabolism in obese type 2 diabetic rats through regulating fibroblast growth factor 21 and its related adipokines[J]. Journal of Traditional Chinese Medicine, 2023 , 43(6) : 1200 -1208 . DOI: 10.19852/j.cnki.jtcm.20231008.002

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