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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 6: 1126 - 1139

DOI: https://doi.org/10.19852/j.cnki.jtcm.20230517.003

Research Articles

Yangqing Chenfei formula (养清尘肺方) alleviates crystalline silica induced pulmonary inflammation and fibrosis by suppressing macrophage polarization

  • Xinrong TIAN ,
  • Runsu HOU ,
  • Xinguang LIU ,
  • Peng ZHAO ,
  • Yange TIAN ,
  • Jiansheng LI
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  • 1 Henan Key Laboratory of Chinese Medicine for Respiratory Disease, Henan University of Chinese Medicine, Zhengzhou 450046, China
    2 Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases co constructed by Henan province and Education Ministry of P.R. China, Zhengzhou 450046, China
    3 Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou 450046, China
    4 Henan Key Laboratory of Chinese Medicine for Respiratory Disease, Henan University of Chinese Medicine, Zhengzhou 450046, China
    5 Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases co constructed by Henan province and Education Ministry of P.R. China, Zhengzhou 450046, China
    6 Department of Respiratory Diseases, the First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000, China

Received date: 2022-08-12

  Accepted date: 2022-11-23

  Online published: 2023-05-17

Supported by

Special Project of Traditional Chinese Medicine Research of Henan Province(2021ZYZD01);Evaluation of the Therapeutic Effect and Characteristics of Traditional Chinese Medicine Dialectical Treatment for Coal Worker's Pneumoconiosis Based on A Multicenter, Randomized, Double-Blind, Parallel Controlled Trial; National Natural Science Fund of China(81973822);Exploring the Mechanism of Bufei Yishen Formula Inhibiting Inflammatory Response in the Treatment of COPD Based on Group Allocation Theory

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Abstract

OBJECTIVE: To explore the underlying mechanisms of the effects of Yangqing Chenfei formula (养清尘肺方, YCF) on inflammation and fibrosis in silicosis via inhibition of macrophage polarization.
METHODS: A silicotic rat model was established via a single intratracheal instillation of silica particles on the first day of week 0. Subsequently, YCF was administered intragastrically to silicotic rats during weeks 0-2 and 5-8 twice daily. The mouse-derived alveolar macrophage cell line was used to investigate the mechanisms of YCF in M1/M2 polarization.
RESULTS: YCF treatment effectively inhibited lung pathological changes, including inflammatory cell infiltration and tissue damage, and increased the forced expiratory volume in the first 0.3 s, functional residual capacity, and maximal mid-expiratory flow in weeks 2 and 8. Furthermore, the treatment improved lung functions by upregulating tidal volume, pause increase, and expiratory flow at 50% tidal volume from weeks 5 to 8. Moreover, YCF could significantly suppressed the progression of inflammation and fibrosis, by reducing the levels of inflammatory cytokines, as well as collagen- I and III. YCF treatment also decreased the numbers of macrophages and M1/M2 macrophages and the level of transforming growth factor-β (TGF-β). Additionally, YCF5, the effective substance in YCF, decreased lipopolysaccharide and interferon-γ-induced M1 macrophage polarization in a concentration-dependent manner. The mechanism of anti-M1 polarization might be related to a decrease in extracellular signal-regulated kinase, c-JUN N-terminal kinase, P38, and P65 phosphorylation. Furthermore, YCF5 inhibited interleukin-4-induced M2 macrophages by decreasing the protein and mRNA expressions of arginase-1 and CD206 as well as the levels of profibrotic factors, such as TGF-β and connective tissue growth factor. The mechanisms underlying the anti-M2 polarization of YCF5 were primarily associated with the inhibition of the nuclear translocation of phosphorylated signal transducer and activator of transcription 6 (p-STAT6).
CONCLUSION: YCF significantly inhibits inflammation and fibrosis in silicotic rats probably via the suppression of M1/M2 macrophage polarization mediated by the inhibition of mitogen-activated protein kinase and nuclear factor kappa B signaling pathways and Janus kinase/STAT6 pathways.

Key words： heart failure; risk factors; fur; Yin deficiency; cohort studies

Cite this article

Xinrong TIAN , Runsu HOU , Xinguang LIU , Peng ZHAO , Yange TIAN , Jiansheng LI . Yangqing Chenfei formula (养清尘肺方) alleviates crystalline silica induced pulmonary inflammation and fibrosis by suppressing macrophage polarization[J]. Journal of Traditional Chinese Medicine, 2023 , 43(6) : 1126 -1139 . DOI: 10.19852/j.cnki.jtcm.20230517.003

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