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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 5: 973 - 982

DOI: https://doi.org/10.19852/j.cnki.jtcm.2023.05.003

Efficacy of catgut embedding in Baihui (GV20) and Feishu (BL13) and Pishu (BL20) on lung tissue, brain tissue and blood related indexes in rats with allergic rhinitis of lung deficiency type

  • Heyin HUANG ,
  • Yichen WANG ,
  • Qinxiu ZHANG ,
  • Xiaojuan WU ,
  • Jiang WANG ,
  • Xiangyu Li ,
  • Rui PANG ,
  • Li FAN ,
  • Liu CAO ,
  • Luo TANG
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  • 1 Department of ENT, Sichuan Integrative Medicine Hospital, Chengdu 610041, China
    2 Department of orthopaedics, Sichuan Province Orthopaedic Hospital, Chengdu 610000, China
    3 Department of ENT, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610000, China
    4 Department of ENT, Quxian Traditional Chinese Medicine Hospital, Quxian 635299, China

Received date: 2022-06-12

  Accepted date: 2022-09-07

  Online published: 2023-08-28

Supported by

General Program of the National Natural Science Foundation of China: Regulatory Mechanism of Acupoint Catgut Embedding via Axonal Reflex on Neurogenic Inflammation in Allergic Rhinitis(81273985);General Program of the National Natural Science Foundation of China: the Study on the Mechanism of Acupoint Catgut Embedding Therapy for Allergic Rhinitis Based on the Coupling of "Acupoint Nasal Mucosal Sensory Nerve Dendritic Cells"(81473523);National Science and Technology Support Program for the Twelfth Five-Year Plan: the Study on Clinical Effect and Operation Standard of Catgut Embedding at Acupoints in Treating Allergic Rhinitis(2015BA I04B00)

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Abstract

OBJECTIVE: To investigate the effects of acupoint catgut embedding for 3 weeks on lung tissue, blood immunoglobulin E (IgE) and interleutin-4 (IL-4), brain tissue microglia x-42 (OX-42) and toll-like receptor-2 (TLR-2) in rats with allergic rhinitis of lung deficiency type.
METHODS: Forty-five female Sprague-Dawley rats were randomly divided for two times. The first time, they were randomly divided into model group and blank group (Group C) according to 2:1, and the second time, the model group were randomly divided into model control group (Group B) and intervention treatment group (Group A) according to 1:1. 15 in each group. For Group A and Group B, the lung deficiency model was made by "sulfur-moxa fumigation", and then the allergic rhinitis model was established by "ovalbumin (OVA) sensitization". Then catgut embedding was performed at acupoints in Group A and not in Group B. After 3 weeks, collect lung tissue samples for hematoxylin-eosin staining, then take blood to observe the concentration of IgE and IL-4, and finally take brain tissue to observe the results of OX-42 and TLR-2.
RESULTS: IgE level (μg/mL) was (3.11 ± 0.20) in the Group A, (4.19 ± 0.44) in the Group B, and (2.29 ± 0.30) in the Group C (all P < 0.001). IL-4 level (pg/mL) was (14.2 ± 0.7) in the Group A, (18.6 ± 2.4) in the Group B, and (11.4 ± 1.2) for the Group C (all P < 0.001). The mean OD for OX-42 is (0.1728 ± 0.0016) in the Group A, (0.1810 ± 0.0046) in the Group B and (0.1674 ± 0.0025) in the Group C (all P < 0.001).
CONCLUSION: Although 3 weeks of acupoint catgut embedding already showed obvious efficacy on rats with allergic rhinitis, the allergic reaction in the body still continued. To achieve further treatment, prolonging the catgut embedding time is necessary.

Key words： rhinitis, allergic; acupoint catgut embedding; enzyme-linked immunosorbent assay; immunoglobulin E; interleukin-4; hippocampus; toll-like receptor 2

Cite this article

Heyin HUANG , Yichen WANG , Qinxiu ZHANG , Xiaojuan WU , Jiang WANG , Xiangyu Li , Rui PANG , Li FAN , Liu CAO , Luo TANG . Efficacy of catgut embedding in Baihui (GV20) and Feishu (BL13) and Pishu (BL20) on lung tissue, brain tissue and blood related indexes in rats with allergic rhinitis of lung deficiency type[J]. Journal of Traditional Chinese Medicine, 2023 , 43(5) : 973 -982 . DOI: 10.19852/j.cnki.jtcm.2023.05.003

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