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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 5: 963 - 972

DOI: https://doi.org/10.19852/j.cnki.jtcm.20230608.001

Electroacupuncture stimulating Zhongji (CV3), Guanyuan (CV4), and bilateral Dahe (KI12) attenuates inflammation in rats with chronic nonbacterial prostatitis induced by estradiol through inhibiting toll-like receptor 4 pathway

  • Zhihao LI ,
  • Wenjun HAN ,
  • Xiuling SONG ,
  • Yan LI ,
  • Yuelai CHEN
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  • 1 Department of Acupuncture-Moxibustion, Tuina and Traumatology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University Shool of Medicine, Shanghai 200233, China
    2 Sleep Medical Center, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    3 Department of Andrology, the Seventh People's Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China
    4 Graduate School of Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

Received date: 2022-05-22

  Accepted date: 2022-08-15

  Online published: 2023-06-08

Supported by

Program of Shanghai Pudong New Area Famous Traditional Chinese Medicine(PWRzm2020-01)

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Abstract

OBJECTIVE: To investigate the anti-inflammatory effect of electroacupuncture (EA) on chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS), also known as chronic nonbacterial prostatitis (CNP), and explore its underlying mechanism.
METHODS: A CNP rat was established by surgical castration combined with 17-β estradiol injection in male Sprague-Dawley rats for thirty consecutive days. The CNP rats received EA treatment once a day for eight days. Chronic pelvic pain was evaluated by mechanical withdrawal threshold measurement. The histological change was assessed by hematoxylin-eosin staining. The inflammatory cytokines in prostates were determined by enzyme-linked immunosorbent assays. The expressions of toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), inhibitors of kappa‐B alpha (IκBα), and nuclear factor-kappa B (NF-κB) were detected by Western blotting. The nuclear translocation of NF-κB and the location of TLR4 were observed with immunofluorescence staining.
RESULTS: The results showed that EA decreased the prostate index, upregulated the mechanical withdrawal threshold, restored the histomorphology of the prostate, reduced the inflammatory factor levels, inhibited NF-κB p65 nuclear translocation, and downregulated the expression levels of critical proteins involved in the TLR4/NF-κB signaling pathway in prostates.
CONCLUSIONS: Our findings suggested that EA could relieve pelvic pain and attenuate prostatic inflammation in estradiol-induced CNP rats. The underlying mechanism may be related to the inhibition of the TLR4/NF-κB signaling pathway.

Key words： chronic nonbacterial prostatitis; electroacupuncture; morphology; anti-inflammatory agents; toll-like receptors

Cite this article

Zhihao LI , Wenjun HAN , Xiuling SONG , Yan LI , Yuelai CHEN . Electroacupuncture stimulating Zhongji (CV3), Guanyuan (CV4), and bilateral Dahe (KI12) attenuates inflammation in rats with chronic nonbacterial prostatitis induced by estradiol through inhibiting toll-like receptor 4 pathway[J]. Journal of Traditional Chinese Medicine, 2023 , 43(5) : 963 -972 . DOI: 10.19852/j.cnki.jtcm.20230608.001

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