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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 5: 906 - 914

DOI: https://doi.org/10.19852/j.cnki.jtcm.20221206.004

Ginsenoside Rb1 alleviates chronic intermittent hypoxia-induced diabetic cardiomyopathy in db/db mice by regulating the adenosine monophosphate-activated protein kinase/Nrf2/heme oxygenase-1 signaling pathway

  • Bingbing LIU ,
  • Jieru LI ,
  • Jianchao SI ,
  • Qi CHEN ,
  • Shengchang YANG ,
  • Ensheng JI
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  • 1 Department of Physiology, Hebei University of Chinese Medicine, Shijiazhuang 050200, China
    2 Hebei Technology Innovation Center of TCM Combined Hydrogen Medicine, Shijiazhuang 050200, China

Received date: 2022-04-07

  Accepted date: 2022-08-08

  Online published: 2022-12-06

Supported by

Natural Science Foundation of Hebei Province: the Central Regulatory Mechanism of Tanshinone IIA on Angiotensin Ⅱ Induced Sympathetic Excitation in Intermittent Hypoxia(H2019423136);Fundamental Research Funds for the Provisional Universities of Hebei University of Chinese Medicine: a Study on the Protective Mechanism of Hydrogen on Chronic Intermittent Hypoxia-induced Microglia Inflammatory Response(YTZ2019001);a Study on the Molecular Mechanism of Improving Effect Danggui Buxue Decoction on Vascular Endothelial Cell Senescence in Chronic Intermittent Hypoxia through Nrf2/HO-1 Signaling Pathway(YXTD2021005);Graduate Innovation Fund of Hebei University of Chinese Medicine: the Effect of Jinlida Granules on the Myocardial Improvement of Chronic Intermittent Hypoxia Aggravated Diabetic Cardiomyopathy in db/db Mice(XCXZZBS2022013)

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Abstract

OBJECTIVE: To examine the protective effect of ginsenoside Rb1 (Rb1), the main component of Renshen (Radix Ginseng), on cardiomyopathy in db/db mice exposed to chronic intermittent hypoxia (CIH) and explore the potential underlying mechanism of Rb1 in treating diabetic cardiomyopathy (DCM).
METHODS: The db/db mice were randomly separated into five groups: normal control group, model group, Rb1 20 mg/kg group, Rb1 40 mg/kg group, and glucagon-like peptide-1 (GLP-1) group. Mice were exposed to air-condition or CIH for 8 weeks, and Rb1 and GLP-1 were administrated before CIH exposure every day. Oral glucose tolerance test (OGTT), intraperitoneal insulin tolerance test (IPITT), total cholesterol (TC), triglyceride (TG), and high-density lipoprotein cholesterol (HDL-C) were detected to evaluate glycolipid metabolism. The level of insulin was detected by a mouse enzyme-linked immunosorbent assay (ELISA). Cardiac function was detected by echocardiography, and myocardial pathology was observed by hematoxylin-eosin and Masson staining. The expression of collagen Ⅰ and collagen Ⅲ was detected by immunohistochemistry. Adenosine monophosphate-activated protein kinase (AMPK)/Nrf2/heme oxygenase-1 (HO-1) signaling pathway was detected by Western blot and immunofluorescence.
RESULTS: Rb1 treatment could improve glucose tolerance and the level of cardiac function indexes, and inhibit the level of oxidative stress indexes and the expression of collagen Ⅰ and collagen Ⅲ. Moreover, Rb1 treatment enhanced AMPK phosphorylation and increased Nrf2 and HO-1 expression.
CONCLUSION: Rb1 treatment alleviated CIH-induced diabetic cardiomyopathy and glycolipid metabolism disorders in db/db mice by inhibiting oxidative stress and regulating the AMPK/Nrf2/HO-1 signaling pathway.

Key words： ginsenosides; diabetic cardiomyopathies; oxidative stress; AMP-activated protein kinases; heme oxygenase-1; signal transduction; chronic intermittent hypoxia

Cite this article

Bingbing LIU , Jieru LI , Jianchao SI , Qi CHEN , Shengchang YANG , Ensheng JI . Ginsenoside Rb1 alleviates chronic intermittent hypoxia-induced diabetic cardiomyopathy in db/db mice by regulating the adenosine monophosphate-activated protein kinase/Nrf2/heme oxygenase-1 signaling pathway[J]. Journal of Traditional Chinese Medicine, 2023 , 43(5) : 906 -914 . DOI: 10.19852/j.cnki.jtcm.20221206.004

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